Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Fluorogenic and Chromogenic Assay for Rapid Detection of Escherichia coli and Total Coliform Bacteria

효소발색법을 이용한 대장균 및 총대장균군 신속 검사

  • 이근헌 ((주)휴마스 부설연구소) ;
  • 김훈수 ((주)휴마스 부설연구소) ;
  • 김병렬 ((주)휴마스 부설연구소) ;
  • 이승희 (한국생명공학연구원 바이오산업화 공정개발센터) ;
  • 인치경 (충남보건환경연구원 폐기물분석과) ;
  • 박경량 (한남대학교 생명공학과)
  • Received : 2008.11.28
  • Accepted : 2009.01.29
  • Published : 2009.05.30
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Abstract

We developed the Eco medium for Escherichia coli and total coliforms, which was modified by Violet Red Bile (VRB) medium, and derived the standard curve of exponential phase at $OD_{410}$ by using type strains such as E. coli ATCC11303, Enterobacter cloacae KCTC2361, Klebsiella pneumoniae KCTC2241, and Citrobacter freundii KCTC2359. Also, we used total 93 samples of spring and stream water to compare the detection ability of total coliforms between the method using Eco medium and such as most probable number (MPN), and plate count methods. As a result, the qualitative analysis of E. coli and total coliforms using Eco medium contained ortho-nitrophenyl-$\beta$-galactoside (ONPG) and 4-methylumbelliferyl-$\beta$-D-glucuronide (MUG) was same as those of Korean standard methods (Colilert kit). And the colony forming unit (CFU) detected in Eco medium was similar to those of result from MPN and plate count methods. Moreover, the agreement, sensitivity, and specificity of the developed kit was more than 97.5% in comparison with Colilert kit for 350 samples. Thus, the Eco medium can be used both qualitative and quantitative analysis of E. coli and total coliforms.

Keywords

Acknowledgement

Supported by : 환경부

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