Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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The Robust Phylogeny of Korean Wild Boar (Sus scrofa coreanus) Using Partial D-Loop Sequence of mtDNA

  • Cho, In-Cheol (Jeju Sub-Station, National Institute of Animal Science, Rural Development Administration) ;
  • Han, Sang-Hyun (Jeju Sub-Station, National Institute of Animal Science, Rural Development Administration) ;
  • Fang, Meiying (College of Animal Science and Technology, China Agricultural University) ;
  • Lee, Sung-Soo (Jeju Sub-Station, National Institute of Animal Science, Rural Development Administration) ;
  • Ko, Moon-Suck (Jeju Sub-Station, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hang (Conservation Genome Resource Bank for Korean Wildlife, Brain Korea 21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Lim, Hyun-Tae (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Yoo, Chae-Kyoung (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University) ;
  • Lee, Jun-Heon (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University)
  • Received : 2009.06.17
  • Accepted : 2009.09.04
  • Published : 2009.11.30
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Abstract

In order to elucidate the precise phylogenetic relationships of Korean wild boar (Sus scrofa coreanus), a partial mtDNA D-loop region (1,274 bp, NC_000845 nucleotide positions 16576-1236) was sequenced among 56 Korean wild boars. In total, 25 haplotypes were identified and classified into four distinct subgroups (K1 to K4) based on Bayesian phylogenetic analysis using Markov chain Monte Carlo methods. An extended analysis, adding 139 wild boars sampled worldwide, confirmed that Korean wild boars clearly belong to the Asian wild boar cluster. Unexpectedly, the Myanmarese/Thai wild boar population was detected on the same branch as Korean wild boar subgroups K3 and K4. A parsimonious median-joining network analysis including all Asian wild boar haplotypes again revealed four maternal lineages of Korean wild boars, which corresponded to the four Korean wild boar subgroups identified previously. In an additional analysis, we supplemented the Asian wild boar network with 34 Korean and Chinese domestic pig haplotypes. We found only one haplotype, C31, that was shared by Chinese wild, Chinese domestic and Korean domestic pigs. In contrast to our expectation that Korean wild boars contributed to the gene pool of Korean native pigs, these data clearly suggest that Korean native pigs would be introduced from China after domestication from Chinese wild boars.

Keywords

Acknowledgement

Supported by : Rural Development Administration, Ministry of Education, Science and Technology

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