The Study of Bone Healing Response According to the Various Surfaces of the Implant in the Mandible of the Mature Dog

성견 하악골에 식립한 다양한 임플란트 표면에서의 골 치유 양상에 관한 연구

  • Lim, Hyun-Pil (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Kim, Yong-Hak (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Vang, Mong-Sook (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Yang, Hong-So (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Park, Sang-Won (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Choi, Hong-Ran (Department of Oral Pathology, School of Dentistry, Chonnam National University)
  • 임현필 (전남대학교 치의학전문대학원 보철학교실) ;
  • 김용학 (전남대학교 치의학전문대학원 보철학교실) ;
  • 방몽숙 (전남대학교 치의학전문대학원 보철학교실) ;
  • 양홍서 (전남대학교 치의학전문대학원 보철학교실) ;
  • 박상원 (전남대학교 치의학전문대학원 보철학교실) ;
  • 최홍란 (전남대학교 치의학전문대학원 구강병리학교실)
  • Received : 2009.01.22
  • Accepted : 2009.05.25
  • Published : 2009.06.30

Abstract

There are many reports that these implant surface treatments may affect to cellular reaction in the surface of implant. This study was done by installing the 8 type implant with the variable surface treatment, used or developed, in the mandible of the mature dog to evaluate how the method of surface treatment of the implant can affect to the bone healing by analyzing histologically and histomorphometrically and find out bone healing appearance periodically after installing implant. By using the 8 type implants which have the different surface treatment, 72 implants were installed on the mandible of 9 mature dogs, and 3 dogs were sacrificed on every 2, 4, 8 weeks. After making bone fragment by cutting and managing, we analyzed histologically, then compared with BIC(Bone to implant contact) for the histomorphometrical analysis. In the result of histological analysis, there was large amount of bone formation in good state on the adjacent area of implant in the 2 weeks testing group. At 4 weeks, although there was general bone formation, the new bone was separated with the basal bone. At 8 weeks, the new bone became matured and connected tightly to the basal bone. There was no difference in the each surface of 8 implants. In the result of histomorphometrical analysis, 2 weeks group had considerably lower value than 4 and 8 weeks group, and there was no difference between 4 and 8 weeks group. There was no difference in the each surface treatment of implants.

성공적인 임플란트 시술을 위해 최근에는 다양하게 표면 처리된 임플란트가 소개되고 있으며, 이러한 표면 처리가 임플란트 표면에서 세포 반응에 영향을 준다는 많은 보고가 있다. 본 연구는 성견 하악골에 현재 사용 중이거나 개발 중인 다양하게 표면 처리된 8종류의 임플란트를 식립하여 조직학적 및 조직계측학적 분석을 함으로써 임플란트 표면 처리 방법이 골 치유에 어떤 영향을 주는지 비교 평가하고 임플란트 식립 후 시기에 따른 골내 치유 양상을 알아보고자 하였다. 8가지의 서로 다르게 표면 처리된 임플란트를 이용하여 총 72개의 임플란트를 9마리의 성견 하악골에 식립하였고, 2주, 4주, 8주에 각각 3마리씩 희생하였다. 골편을 절단 및 처리하여 시편을 제작한 후 조직학적 분석을 하였으며, 조직계측학적 분석으로 bone to implant contact(BIC)를 측정 비교하였다. 조직학적 분석 결과 전반적으로 골 형성이 좋은 상태로 2주의 실험군에서도 임플란트의 인접부위에서 상당량의 골 형성을 볼 수 있었다. 4주의 실험군에서는 골 형성이 전반적으로 관찰되었으나, 신생골은 기저골과 구분되었고, 8주 실험군에서는 신생골이 성숙되어 기저골과 잘 연결되어 있었다. 8가지 서로 다른 임플란트에서 표면에 따른 차이는 보이지 않았다. 조직계측학적 분석 결과 2주군에서 4주군과 8주군에 비해 BIC가 유의할만하게 낮게 나타났으며 4주군과 8주군 사이에는 유의성 있는 차이를 보이지 않았다, 또한 표면 처리에 따른 차이도 보이지 않았다.

Keywords

References

  1. Ericsson I, Johansson CB, Bystedt H, Norton MR. A histomorphometric evaluation of bone-to-implant contact on machine-prepared and roughened titanium dental implants: A pilot study in the dog. Clin Oral Implants Res 1994;5(4):202-206 https://doi.org/10.1034/j.1600-0501.1994.050402.x
  2. Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R. Enhanced functions of osteoblast on nanophase ceramics. Biomaterials 2000;21:1803-10 https://doi.org/10.1016/S0142-9612(00)00075-2
  3. Webster TJ, Schandler LS, Siegel RW, Bizios R. Mechanics of enhanced osteoblast adhesion on nanophase alumina involve vitronectin. Tissue Eng 2001;7:291-310 https://doi.org/10.1089/10763270152044152
  4. Meredith, N., Shagaldi, F., alleyne, D., Sennerby, L., Cawley, P. The application of resonance frequency measurements to study the stability of titanium implants during healing in the rabbit tibia. Clin Oral Implants Res 1997b;8:234-243 https://doi.org/10.1034/j.1600-0501.1997.080310.x
  5. Sennerby L. Roos J Surgical determinants of clinical success of osseointegrated Prosthodont 1998;11:408- 420
  6. Sennerby L. Roos J. RFA: Measuring implant stability and osseointegration. Compendium 1998;19: 493-502
  7. Sennerby L. Friberg B. Linden B. Jemt T. Meredith N. A comparison of implant bone using RFA:European commission demonstration Project. RFA Symposium. 2002 Sweden
  8. Park CJ. A study on the change of implant stability using resonance frequency analysis SNUDH Dept of Pros. Unpublished 2003
  9. Berglundh T, Abrahamsson I, Lang NP, Lindhe J. De novo alveolar bone formation adjacent to endosseous implants. Clin Oral Implants Res 2003;14:251-262 https://doi.org/10.1034/j.1600-0501.2003.00972.x
  10. Misch CE. Density of bone: Effect on treatment plans, surgical approach, healing, and progressive bone loading. Int J Oral IMplantol 1990;6:23-31
  11. Sun L. Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: A review. J Biomed Mater Res 2001;58: 570-592 https://doi.org/10.1002/jbm.1056
  12. Tal H. Spontaneous early exposure of submerged implants.Ⅱ: Histopathology and histomorphometry of non-perforated mucosa covering submerged implants. J Periodontol 2000;71:1231-1235 https://doi.org/10.1902/jop.2000.71.8.1231
  13. Uchida M, Kim HM, Kokubo T, Fujibayashi S, Makamura T. Structural dependence of apatite formation on titania gels in a simulated body fluid. J Biomed Mater Res 2003;64:164-70
  14. Li D, Rerguson SJ, Beutler T, Cochran DL, Sittig C, Hirt HP, Buser D. Biomechanical coparison of the sandblasted and acid-etched and the achined and acid-etched titanium surface for dental implants. J Biomed Mater Res. 2002;60:325-332 https://doi.org/10.1002/jbm.10063
  15. Cochran DL, Schenk RK, Lussi A, Higginbottom FL, Buser D, Bone response to unloaded and loaded titanium implants with a sandblasted and acid-etched surface: A histometric study in the canine mandible. J Biomed Mater Res 1998;40:1-11 https://doi.org/10.1002/(SICI)1097-4636(199804)40:1<1::AID-JBM1>3.0.CO;2-Q
  16. Rocci A, Martignoni M, Burgos PM, Gottlow J, Sennerby L. Histology of retrieved immediately and early loaded oxidized implants: light microscopic observations after 5 to 9 months of loading in the posterior mandible. Clin Implant Dent Related Res 2003;5(Suppl 1):88-98 https://doi.org/10.1111/j.1708-8208.2003.tb00020.x
  17. Parel SM, Triplett RG. Immediate fixture placement: a treatment planning alternative. Int J Oral Maxillofac Implants 1990;5(4):337-345
  18. Meredith N, Friberg B, Sennerby L. Araricio C. Relationship between contact time measurements and PTV values when using the Periotest to measure implant stability. Int J Prosthodontics 1998;11: 269-275
  19. Sennerby L. Resonance frequency measurements of implant stability in vivo: A cross-sectional and longitudinal study of resonance frequency measurements on implants in the edentulous and partially dentate maxilla. Clin Oral Implants Res 1997;8:226-333
  20. Derhami K, Wolfaardt JF, Dent M, Faulkner G. Grace M. Assessment of the Periotest device in baseline mobility measurements of craniofacial implants. Int J Oral Maxillofac Implants 1995;10:221-229
  21. Schatzker J, Horne JG, Sumnet SG. The effect of movement on holding power of screws in bone. Clin Orthop 1975;111:257-262 https://doi.org/10.1097/00003086-197509000-00032
  22. Papalexiou V, Novaes AB Jr, Grisi MFM, Souza SSLS. Influence of implant microstructure on the dynamics bone healing round immediate implants placed into periodontally infected sites. Clin Oral Impl Res 2004;15:44-53 https://doi.org/10.1111/j.1600-0501.2004.00995.x