한국어병학회지 (Journal of fish pathology)

  • 제22권3호
  • /
  • Pages.317-326
  • /
  • 2009
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Antimicrobial and antioxidant activity of some Indian medicinal plants for the protection against fish pathogenic bacteria

  • Harikrishnan, Ramasamy (Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences & Marine and Environmental Reseach Institute College of Ocean Science, Jeju National University) ;
  • Jawahar, Sundaram (Department of Biotechnology, Bharath College of Science and Management) ;
  • Kim, Man-Chul (Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences & Marine and Environmental Reseach Institute College of Ocean Science, Jeju National University) ;
  • Kim, Ju-Sang (Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences & Marine and Environmental Reseach Institute College of Ocean Science, Jeju National University) ;
  • Jang, Ik-Soo (Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences & Marine and Environmental Reseach Institute College of Ocean Science, Jeju National University) ;
  • Balasundaram, Chellam (Department of Animal Science, School of Life Sciences, Bharathidasan University) ;
  • Heo, Moon-Soo (Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences & Marine and Environmental Reseach Institute College of Ocean Science, Jeju National University)
  • 발행 : 2009.12.01
⟨ 이전 논문 다음 논문 ⟩

초록

This study has shown the screening of anti-bacterial activity of three Indian medicinal plant choloroform : methanol (50:50) solvent leaf extracts (i.e. Azadirachta indica, Ocimum sanctum, and Curcuma longa) with different concentrations (10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/ml) under in vitro conditions against fish pathogenic bacteria, Aeromonas hydrophila, Streptococcus iniae, Vibrio harveyi, V. anguillarum, and Edwardsiella tarda isolated from olive flounder farms, Jeju Island, South Korea. The anti-microbial activity of the A. indica and O. sanctum extracts yielded the zones of growth inhibition (ZI) was 3 and 1mm against A. hydrophila at concentration of 0.156 mg/ml when compared to that of tetracycline standard (3 mm). At highest concentration (10 mg/ml) of A. indica, O. sanctum, and C. longa, high inhibition was 9, 7, and 6 mm when compared to that of tetracycline (11 mm) against A. hydrophila. The minimum inhibitory concentration (MIC) of A. indica, O. sanctum, and C. longa at 0.156 mg/ml that yield 9, 10, and 13 CFU/ml for A. hydrophila, 16, 22, and 25 CFU/ml for S. iniae and 18, 22, and 23 CFU/ml for E. tarda compared to the tetracycline. At highest concentration (10 mg/ml) of the three extracts was better inhibiting the growth of A. hydrophila, S. iniae and E. tarda. A. indica, O. sanctum, and C. longa were determined to the potential antioxidant activityon the basis of their scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. A. indica extract was 0.625 mg/ml which indicated that the strong anti-oxidant activity. However, O. sanctum and C. longa extracts showed weak anti-oxidant activity at this concentration. Hence, in vitro assay among the pathogens, A. hydropila is better inhibitory activity of the extracts. It is evident that the Indian medicinal plants extracts were subjected to its effectiveness against A. hydrophila, S. iniae, and E.tarda at low concentrations. The obtained results in the present study suggested that the Indian plant extracts is a prevention tools for Korean olive flounder aquaculture pathogens and its need further advance investigation.

키워드

참고문헌

  1. Adams, A., Auchinachie, N., Bundy, A., Tatner, M.F. and Horne, M.Y.: The potency of adjuvanted vaccines in rainbow trout, Salmo gairdneri Richardson, with whole cell bacterins by oral and intraperitoneal routes. J. Fish Dis., 6:129-134, 1998 https://doi.org/10.1111/j.1365-2761.1983.tb00060.x
  2. Alves, T.M.A., Silva, A.F., Brandao, M., Grandi, T.S.M., Smania, E.F.A., Smania Jr A. and Zani, C.L.: Biological screening of Brazilian medicinal plants. The J Memorias do Instituto Oswaldo Cruz, 95:367-373, 2000
  3. Anderson, D.P.: Immunostimulants, adjuvants, and vaccine carriers in fish: applications in aquaculture. Ann. Rev. Fish Dis., 2: 281-301, 1992 https://doi.org/10.1016/0959-8030(92)90067-8
  4. Anto, R.J., George, J., Dinesh-Babu, K.V., Rajasekharan, K.N. and Kuttan, R.: Antimutagenic and anticarcinogenic activity of natural and synthetic curcuminoids. Mutation Res., 370:127-131, 1996 https://doi.org/10.1016/0165-1218(96)00074-2
  5. Babu, B., Jisha, V.K., Salitha, C.V., Mohan, S. and Valsa, A.K.: Antibacterial activity of different plant extracts. Indian J. Microbiol., 42:361-363, 2000
  6. Baeck, G.W., Kim, J.H., Gomez, D.K. and Park, S.C.: Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju Island. J. Vet. Sci., 7:53-58, 2006
  7. Bauer, A.W., Kirby, M. and Shenis, J.C.: Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clim. Pathol., 45:493-497, 1966
  8. Bowden, T.J., Adamson, K., MacLachlan, P., Pert, C.C. and Bricknell, I.: Long-term study of antibody response and injection-site effects of oil adjuvants in Atlantic halibut (Hippoglossus hippoglossus L.). Fish Shellfish Immunol., 14:363-369, 2003 https://doi.org/10.1006/fsim.2002.0439
  9. Collins, C.H., Lyne, P.M. and Grange, J.M.: Microbiological methods, 6th edition. Butterworth, London, 1989
  10. Cox, J.C. and Coulter, A.R.: Adjuvantsda classification and review of their modes of action. Vaccine, 15:248-256, 1997 https://doi.org/10.1016/S0264-410X(96)00183-1
  11. Cuendet, M., Hostettmann, K. and Potterat, O.: Iridoid glucosides with free radical-scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80:1144-1152, 1997 https://doi.org/10.1002/hlca.19970800411
  12. Devakumar, C. and Sukh Dev.: Chemistry, In: Neem (ed. By N.S. Randhawa, and B.S. Parmar), pp.77-110, 2nd edition, Society of Pesticide Science, New Delhi, India, 1996
  13. Evans, W.C.: Trease and Evans' Pharmacognosy', 14th edition, W.B. Saunders Co. Ltd., London, 1996
  14. Gupta, S.K., Prakash, J. and Srivastava, S.: Validation of traditional claim of Tulsi Ocimum sanctum Linn. as a medicinal plant. Indian J. Exp. Biol., 40:765-773, 2002
  15. Harikrishnan, R. and Balasundaram, C.: Antimicrobial activity of medicinal herbs in vitro against fish pathogen, Aeromonas hydrophila. Fish Pathol., 40:187-189, 2005 https://doi.org/10.3147/jsfp.40.187
  16. Harikrishnan, R., Rani, N.M. and Balasundaram, C.: Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture, 221:41-50, 2003 https://doi.org/10.1016/S0044-8486(03)00023-1
  17. Hunter, P.A. and Reeves, D.S.: The current status of surveillance of resistance to antimicrobial agents: report on a meeting. J. Antimicrob. Chemother., 49:17-23, 2002 https://doi.org/10.1093/jac/49.1.17
  18. Iwalokun, B.A., Gbenle, G.O., Adewole, T.A. and Akinsinde, K.A.: Shigellocidal properties of three Nigerian medicinal plants: Ocimum gratissimum, Terminalia avicennoides, and Momordica balsamina. J. Health Popul. Nutr., 19:331-335, 2001
  19. Janssen, A.M., Scheffer, J.J., Ntezurubanza, L. and Baerheim Svendsen, A.: Antimicrobial acitivities of some Ocimum species grown in Rwanda. J. Ethnopharmacol., 26:57-63, 1989 https://doi.org/10.1016/0378-8741(89)90113-X
  20. Joseph, S.W. and Carnahan, A.: The isolation, identification, and systematics of the motile Aeromonas species. Ann. Rev. Fish Dis., 4:315-343, 1994 https://doi.org/10.1016/0959-8030(94)90033-7
  21. Kikuzaki H. and Nakatani, N.: Antioxidant Effects of Ginger Constituents. J. Food Sci., 58:1407-1410, 1993 https://doi.org/10.1111/j.1365-2621.1993.tb06194.x
  22. Kim, D.H., Han, H.J., Kim, S.M., Lee, D.C. and Park, S.I.: Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J. Fish Dis., 27:497-505, 2004 https://doi.org/10.1111/j.1365-2761.2004.00553.x
  23. Larson, R.A.: The antioxidants of higher plants. Phytochemistry, 27:969-978, 1988 https://doi.org/10.1016/0031-9422(88)80254-1
  24. Lawrence, G.W., Saul, A., Giddy, A.J., Kemp, R. and Pye, D.: Phase I trial in humans of an oil-based adjuvant SEPPIC MONTANIDE ISA 720. Vaccine, 15:176-178, 1997 https://doi.org/10.1016/S0264-410X(96)00150-8
  25. Lee, H.K., Son, B.H. and Oh, M.J.: Microbial flora in ascetic cultivated olive flounder (Paralichthys olivaceus) in Koe-je island in Korea during 2002-2003. Korean J. Microbiol., 40:133-138, 2004
  26. Logambal, S.M. and Michael, R.D.: Immunostimulatory effect of Azadirachtin in Oreochromis mossambicus (Peters). Indian J. Exp. Biol., 38:1092-1096, 2000
  27. Mandal, S., Mandal, M., Pal, N.K. and Halder, P.K.: R factor in Proteus vulgaris from ulcerative disease of fish, Channa punctatus. Indian J. Exp. Biol., 40:614-616, 2002
  28. Mitra, C.R., Garg, H.S. and Pandey, G.N.: Identification of nimbidic acid and nimbidinin from Azadirachta indica. Photochemistry, 10:857-860, 1971 https://doi.org/10.1016/S0031-9422(00)97156-5
  29. Mukherjee, D., Guha, D., Kumar, V. and Chakraborty, S.: lmpairment of steroidgenesis and reproduction in sexually mature Cyprinus carpio by phenol and sulfide under laboratory conditions. Aquat. Toxicol., 21:29-40, 1991 https://doi.org/10.1016/0166-445X(91)90004-S
  30. Nakamura, C.V., Ueda-Nakamura, T., Bando, E., Melo,A.F.N., Cortez, D.A.G. and Dias Filho, B.P.: Antibacterial activity of Ocimum gratissimum L essential oil. J Memorias do Instituto Oswaldo Cruz, 94:675-678, 1999
  31. National Committee for Clinical Laboratory Standards.: Methods for dilution antimicrobial susceptibilitytests for bacteria that grow aerobically. 3rd ed. Approved Standard. Document M7-A4. Natl. Committee Clin. Lab. Stand., Wayne, PA, 1997
  32. National Committee for Clinical Laboratory Standards.: Performance standards for antimicrobial susceptibility testing: tenth informational supplement M100-S10. NCCLS, Wayne, PA, 2000
  33. Njiro, S. M. and Kofi-Tsekpo, M.V.: Effect of an aqueous extract of Azadirachta indica on the immune response in mice. Onderstepoort. J. Vet. Res., 66:59-62, 1999
  34. Oh, M.J., Kim, W.S., Kitamura, S.I., Lee, H.,Son, B.W., Jung, T.S. and Jung, S.J.: Change of pathogenicity in olive flounder Paralichthys olivaceus by co-infection of Vibrio harveyi, Edwardsiella tarda and marine birnavirus. Aquaculture, 257:156-160, 2005 https://doi.org/10.1016/j.aquaculture.2006.03.009
  35. Pike, J. and Chandra, R.K.: Effect of vitamin and trace element supplementation on immune indices in healthy elderly. Int. J. Vitam. Nutr. Res., 65:117-120, 1995
  36. Rasadah, M.A. and Houghton, P.J.: Antimicrobial activity of some species of Bignoniaceae. ASEAN Review of Biodiversity and Environmental Conservation (ARBEC), article III. Available: www.arbec.com.my/pdf/may-3.pdf, 2007
  37. Reddy, R.C., Vatsaala, P.G., Keshamouni, V.G., Padmanaban, G. and Rangarajan, P.N.: Curcumin for malaria therapy. Biochem. Biophys. Res. Commun., 326:472-474, 2005 https://doi.org/10.1016/j.bbrc.2004.11.051
  38. Rojanapo, W., Suwanno, S., Somaree, R., Glinsukon, T. and Thebtaranonth, Y.: Carcinogenicity of an oxidation product of pphenylenediamine J. Sci. Thailand, 11:177-188, 1985 https://doi.org/10.2306/scienceasia1513-1874.1985.11.177
  39. Sadhu, S.K., Okuyama, E., Fujumoto, H. and Ishibashi, M.: Seperartion of leucas, aspara amedicinal plant of Bangladesh, guided by prostaglandin inhibitory and anti oxidant activities. Chem. Pharm. Bull., 51:595-598, 2003 https://doi.org/10.1248/cpb.51.595
  40. Satoskar, R.R., Shah, S.J. and Shenoy, S.G.: Evaluation if anti-inflammatory properties of curcumin (diferuloylmethane) in patients with postoperative inflammation. Int. J. Clim. Pharmacol. Ther. Toxicol., 24:651-654, 1986
  41. Skaltsa, H. and Shammas, G.: Flavonoids from Lippia citriodora. Planta Med., 54:465, 1988 https://doi.org/10.1055/s-2006-962505
  42. Soliman, K.M. and Badeaa, R.I.: Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food Chem. Toxicol., 40:1669-1675, 2002 https://doi.org/10.1016/S0278-6915(02)00120-5
  43. Uddin, S.N., Ali, M.E. and Yesmin, M.N.: Antioxidant and Antibacterial Activities of Senna tora Roxb. Am. J. Plant Physiol., 3:96-100, 2008 https://doi.org/10.3923/ajpp.2008.96.100
  44. Vaidyanathan, P.S.V.: Indian Medicinal plants Arya Vaidya Sala, Kottaail, Orient Longman Ltd, Chennai, 1995
  45. Valentao, P., Fernandes, E., Carvalho, F., Andrade, P.B., Seabra, R.M. and de Lourdes Basto, M.: Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol. Pharm. Bull., 25:1324-327, 2002 https://doi.org/10.1248/bpb.25.1324
  46. Venkatalakshmi, S. and Michael, R.D.: Immunostimulation by leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters). J. Aquacultu. Tropics, 16:1-10, 2001
  47. Wan, J., Wilcock, A. and Coventry, M.J.: The effect of essential oils of basil on the growth of Aeromonas hydrophila and Pseudomonas fluorescens. J. Appl. Microbiol., 84:152-158, 1998 https://doi.org/10.1046/j.1365-2672.1998.00338.x
  48. World Health Organization.:Traditional Medicine Strategy 2002-2005. Geneva: World Health Organization, 2002
  49. Zhang, X.: Traditional medicine: its importance and protection (ed. By S. Twarog, and P. Kapoor) In: Protecting and Promoting Traditional Knowledge: Systems, National Experiences and International Dimensions. Part 1. The Role of Traditional Knowledge in Healthcare and Agriculture. New York: United Nations, pp.3-6, 2004