Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

  • Bhuiyan, M.S.A. (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Yoon, D. (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Cho, Y.M. (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Park, E.W. (Division of Animal Biotechnology, National Institute of Animal Science) ;
  • Kim, N.K. (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Kim, K.S. (Department of Animal Science, Chungbuk National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2008.10.07
  • Accepted : 2009.02.02
  • Published : 2009.06.01
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Abstract

Sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN) genes play an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. This study used polymorphisms in the intron 5 of bovine SREBF1 and in the thioesterase (TE) domain of FASN genes to evaluate their associations with beef fatty acid composition. A previously identified 84-bp indel (L: insertion/long type and S: deletion/short type) of the SREBF1 gene in Korean cattle had significant associations with the concentration of stearic (C18:0), linoleic (C18:2) and polyunsaturated fatty acids (PUFA). The stearic acid concentration was 6.30% lower in the SS than the LL genotype (p<0.05), but the linoleic and PUFA contents were 11.06% and 12.20% higher in SS compared to LL (p<0.05). Based on the sequence analysis, five single nucleotide polymorphisms (SNPs) g.17924G>A, g.18043C>T, g.18440G>A, g.18529G>A and g.18663C>T in the TE domain of the FASN gene were identified among the different cattle breeds studied. Among these, only g.17924 G>A and g.18663C>T SNPs were segregating in the Hanwoo population. The g.17924G>A SNP is a non-synonymous mutation (thr2264ala) and was significantly associated with the contents of palmitic (C16:0) and oleic acid (C18:1). The oleic acid concentration was 3.18% and 2.79% higher in Hanwoo with the GG genotype than the AA and AG genotypes, respectively (p<0.05), whereas the GG genotype had 3.8% and 4.01% lower palmitic acid than in those cattle with genotype AA and AG, respectively (p<0.05). Tissue expression data showed that SREBFI and FASN genes were expressed in a variety of tissues though they were expressed preferentially in different muscle tissues. In conclusion, the 84-bp indel of SREBF1 and g.17924G>A SNP of the FASN gene can be used as DNA markers to select Hanwoo breeding stock for fatty acid composition.

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References

  1. Assaf, S., D. Hazard, F. Pitel, M. Morisson, M. Alizadeh, F. Gondret, C. Diot, A. Vignal, M. Douaire and S. Lagarrigue. 2003. Cloning of cDNA encoding the nuclear form of chicken sterol response element binding protein-2 (SREBP-2), chromosomal localization and tissue expression of chicken SREBP-1and -2 genes. Poult. Sci. 82:54-61
  2. Barendse, W., R. J. Bunch, B. E. Harrison and M. B. Thomas. 2006. The growth hormone GH1: c.457C>G mutation is associated with relative fat distribution in intra-muscular and rump fat in a large sample of Australian feedlot cattle. Anim. Genet. 37:211-214 https://doi.org/10.1111/j.1365-2052.2006.01432.x
  3. Bonanome, A. and S. Grundy. 1988. Effect of dietary stearic acid on plasma cholesterol and lipoprotein concentrations. N. Engl. J. Med. 318:1244-1248 https://doi.org/10.1056/NEJM198805123181905
  4. Brown, M. S. and J. L. Goldstein. 1997. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane bound transcription factor. Cell. 89:331-340 https://doi.org/10.1016/S0092-8674(00)80213-5
  5. Burge, C. and S. Karlin. 1997. Prediction of complete gene structures in human genomic DNA. J. Mol. Biol. 268:78-94 https://doi.org/10.1006/jmbi.1997.0951
  6. Casas, E., S. N. White, T. L. Wheeler, S. D. Shackelford, M. Koohmaraie, D. G. Riley, C. C. Chase, D. D. Johnson and T. P. L. Smith. 2006. Effects of calpastatin and mu-calpain markers in beef cattle on tenderness traits. J. Anim. Sci. 84:520-525
  7. Chakravarty, B., Z. Gu, S. S. Chirala, S. J. Wakil and F. A. Quiocho. 2004. Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain. Proc. Natl. Acad. Sci., USA. 101:15567-15572 https://doi.org/10.1073/pnas.0406901101
  8. Chen, J., X. J. Yang, D. Xia, J. Chen, J. Wegner, Z. Jiang and R. Q. Zhao. 2008. Sterol regulatory element binding transcription factor 1 expression and genetic polymorphism significantly affect intramuscular fat deposition in the longissimus muscle of Erhualian and Sutai pigs. J. Anim. Sci. 86:57-63 https://doi.org/10.2527/jas.2007-0302
  9. Cheong, H. S., D. Yoon, L. H. Kim, B. L. Park, H. W. Lee, C. S. Han, E. M. Kim, H. Cho, E. R. Chung, I. Cheong and H. D. Shin. 2007. Titin-cap (TCAP) polymorphisms associated with marbling score of beef. Meat Sci. 77:257-263 https://doi.org/10.1016/j.meatsci.2007.03.014
  10. Di Statio, L., G. Destefanis, A. Brugiapaglia, A. Albera and A. Ronaldo. 2005. Polymorphism of the GHR gene in cattle and relationship with meat production and quality. Anim. Genet. 36:138-140 https://doi.org/10.1111/j.1365-2052.2005.01244.x
  11. Falconer, D. S. and T. F. C. Mackay. 1996. Introduction to quantitative genetics (4th edn.). Longman, England
  12. Felder, T. K., K. Klein, W. Patsch and H. Oberkofler. 2005. A novel SREBP-1 splice variant: Tissue abundance and transactivation potency. Biochem. Biophys. Acta. 1731:41-47 https://doi.org/10.1016/j.bbaexp.2005.08.004
  13. Hoashi, S., N. Ashida, H. Ohsaki, T. Utsugi, S. Sasazaki, M. Taniguchi, K. Oyama, F. Mukai and H. Mannen. 2007. Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle. Mamm. Genom. 18:880-886 https://doi.org/10.1007/s00335-007-9072-y
  14. Kennes, Y. M., B. D. Murphy, F. Pothier and M. F. Palin. 2001. Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Anim. Genet. 32:215-218 https://doi.org/10.1046/j.1365-2052.2001.00768.x
  15. Kim, J. B. and B. M. Spiegelman.1996. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes. Dev. 10:1096-1107 https://doi.org/10.1101/gad.10.9.1096
  16. Kim, Y. M., J. H. Kim, S. C. Kim, H. M. Ha, Y. D. Ko and C.H. Kim. 2002. Influence of dietary addition of dried wormwood (Artemisia sp.) of the performance, carcass characteristics and fatty acid composition of muscle tissues of Hanwoo Heifers. Asian-Aust. J. Anim. Sci. 15:549-554
  17. Lepage, G. and C. C. Roy. 1986. Notes on methodology: Direct transesterification of all classes of lipids in a one-step reaction. J. Lip. Res. 27:114-120 https://doi.org/10.1371/journal.pone.0010446
  18. Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-\Delta\Delta{C}}T$ method. Methods. 25:402-408 https://doi.org/10.1006/meth.2001.1262
  19. Morris, C. A., N. G. Cullen, B. G. Glass, D. L. Hyndman, T. R. Manley, S. M. Hickey, J. C. McEwan, W. S. Pitchford, C. D. K. Bottema and M. A. H. Lee. 2007. Fatty acid synthase effects on bovine adipose fat and milk fat. Mamm. Genom. 18:64-74 https://doi.org/10.1007/s00335-006-0102-y
  20. Mozaffarian, D., A. Ascherio, F. B. Hu, M. J. Stampfer and W. C. Willett. 2005. Interplay between different polyunsaturated fatty acids and risk of coronary heart disease in man. Circulation 111:257-164
  21. Roy, R., L. Ordovas, P. Zaragoza, A. Romero, C. Moreno, J. Altarriba and C. Rodellar. 2006. Association of polymorphisms in the bovine FASN gene with milk fat content. Anim. Genet. 37:215-218 https://doi.org/10.1111/j.1365-2052.2006.01434.x
  22. Roy, R., M. Gautier, H. Hayes, P. Laurent and R. Osta. 2001. Assignment of the fatty acid synthase (FASN) gene to bovine chromosome 19 (19q22) by in sito hybridization and confirmation by somatic cell hybrid mapping. Cytogenet. Cell. Genet. 93:141-142 https://doi.org/10.1159/000056970
  23. Roy, R., S. Taourit, P. Zaragoza, A. Eggen and C. Rodellar. 2005. Genomic structure and alternative transcript of bovine fatty acid synthase gene (FASN): comparative analysis of the FASN gene between monogastric and ruminant species. Cytogenet. Genom. Res. 111:65-73 https://doi.org/10.1159/000085672
  24. Rozen, S. and H. J. Skaletsky. 2000. Primer 3 on the WWW for general users and for biologist programmers. In: Bioinformatics methods and protocols: Methods in molecular biology (Ed. S. Krawetz and S. Misener). Humana Press, Totowa, NJ. pp. 365-386
  25. Shimano, H., N. Yahagi, M. Amemiya-Kudo, A. H. Hasty, J. Osuga, Y. Tamura, F. Shionoiri, Y. Iizuka, K. Ohashi, K. Harada, T. Gotoda, S. Ishibashi, and N. Yamada. 1999. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. J. Biol. Chem. 274:35832-35839 https://doi.org/10.1074/jbc.274.50.35832
  26. Shimomura, I., H. Shimano, J. D. Horton, J. L. Goldstein and M. S. Brown. 1997. Differential expression of exons 1a and 1c in mRNA for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. J. Clin. Invest. 99:838-845 https://doi.org/10.1172/JCI119247
  27. Shin, S. C. and E. R. Chung. 2007a. Association of SNP marker in the leptin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20:1-6
  28. Shin, S. C. and E. R. Chung. 2007b. Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20:172-177
  29. Shin, S. C. and E. R. Chung. 2007c. SNP detection of carboxypeptidase E gene and its association with meat quality and carcass traits in Korean cattle. Asian-Aust. J. Anim. Sci. 20:328-333 https://doi.org/10.1186/1471-.2156-9-41
  30. Tamura, K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599 https://doi.org/10.1093/molbev/msm092
  31. Thaller, G., C. Kuhn, A. Winter, A. Ewald, O. Bellmann, J.Wegner, H. Zuhlke and R. Fries. 2003. DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim. Genet. 34:354-357 https://doi.org/10.1046/j.1365-2052.2003.01011.x
  32. Whetsell, M. S., E. B. Rayburn and J. D. Lozier. 2003. Human health effects of fatty acids in beef. A report on 'Pasture based beef systems for Appalachia,' West Virginia University, USA
  33. Woollett, L. A., D. K. Spady and J. M. Dietschy. 1992. Saturated and unsaturated fatty acid independently regulate low density lipoprotein receptor activity and production rate. J. Lip. Res. 33:77-78 https://doi.org/10.1172/JCI114131
  34. Yang, A., T. W. Larsen, S. B. Smith and R. K. Tume. 1999. $\Delta^{9}$- desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids. 34:971-978 https://doi.org/10.1007/s11745-999-0447-8
  35. Zhang, S., T. J. Knight, J. M. Reecy and D. C. Beitz. 2008. DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim. Genet. 39:62-70 https://doi.org/10.1111/j.1365-2052.2007.01681.x

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