Characterization of haemocytes in the surf clam Mactra veneriformis

  • Yu, Jin-Ha (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Kyung-Il (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
  • Published : 2009.12.01

Abstract

Haemocyte characterization in the surf clam, Mectra veneriformis was carried out based on morphological, cytochemical, and phagocytic characteristics. The haemocytes were classified into two cell types, granulocytes and agranulocytes on the basis of presence of cytoplasmic granules. Granulocytes were then classified again into 2 types, large eosinophilic granulocytes and small eosinophilic granulocytes after staining with May-Grünwald Giemsa. In electron microscopy, both types of granulocytes contained electron-dense and electron-lucent cytoplasmic granules. Agranulocytes (hyalinocytes) were also divided into two cell types, large agranulocytes and small agranulocytes based on their sizes. Both cell types did not have granules in cytoplasm. Granulocyte and agranulocyte were negative for the enzyme activities of alkaline phosphatase, peroxidase, and $\beta$-glucuronidase but positive for phenoloxidase and acid phosphatase activities. Both types of haemocytes have phagocytic activity, with the exception of small agranulocyte, and granulocytes seemed more active in this respect than agranulocytes. This present study is the first study to characterize haemocytes of M. veneriformis.

Keywords

References

  1. Aladaileh, S., Rodney, P., Nair, S.V. and Raftos, D.A.: Characterization of phenoloxidase activity in Sydney rock oysters (Saccostrea glomerata). Comp. Biochem. Physiol. Part B, 148:470-480, 2007 https://doi.org/10.1016/j.cbpb.2007.07.089
  2. Auffret, M.: Bivalve hemocyte morphology. In: Fisher WS, editor. Disease processes in marine bivalve mollusks. Bethesda: MD American Fishery Society, pp.169-177, 1988
  3. Bachere, E., Chargot, D. and Grizel, H.: Separation of Crassostrea gigashemocytes by density gradient centrifugation and counterflow centrifugal elutriation. Dev. Comp. Immunol., 12:549-559, 1988 https://doi.org/10.1016/0145-305X(88)90071-7
  4. Barracco, M.N., Medeiros, D. and Moreira F.M.: Some hemato-immunological parameters in the mussel Perna perna. Fish Shellfish Immunol., 9:387-401, 1999 https://doi.org/10.1006/fsim.1998.0196
  5. Bayne, C.T., Simnia, T. and Van der Knnap, W.P.: Immunological memory: status of molluscan studies. Phylogeny of immunological memory, Elsivier, pp.57-64, 1980
  6. Carballal, M.J., Lopez, C., Azevedo, C. and Villalba, A.: Hemolymph cell types of the mussel Mytilus galloprovincialis. Dis. Aquatic. Org., 29:127-135, 1997a https://doi.org/10.3354/dao029127
  7. Carballal, M.J., Lopez, C., Azevedo, C. and Villalba, A.: Enzyme involved in defense functions of hemocytes of mussel Mytilus galloprovincialis. J. Invertebr. Pathol., 70:96-105, 1997b https://doi.org/10.1006/jipa.1997.4670
  8. Carballal, M.J., Lopez, C., Azevedo, C. and Villalba, A: In vitro study of phagocytic ability of Mytilus galloprovincialis. Dis. Aqua. Org., 29:127-135, 1997c https://doi.org/10.3354/dao029127
  9. Cajaraville, M.P. and Pal, S.G.: Morpholfunctional study of the hemocytes of the bivalve mollusk Mytilus galloprovincialis with emphasis on the endolysosomal compartment. Cell. Strut funct., 20:355-367, 1995 https://doi.org/10.1247/csf.20.355
  10. Cheng, T.C.: Beta-glucuronidase in the serum and hemolymph cells of Mercenaria mercenaria and Crassostrea virginica (Mollusca: Pelecypoda). J. Invertebr. Pathol., 27:125-128, 1976 https://doi.org/10.1016/0022-2011(76)90036-7
  11. Cheng, T.C.: Invertebrate blood cells 1. Bivalves. Academic Press, pp.233-300, 1981
  12. Chou, H.Y., Chang, S.J. and Tseng, S.M.: Morphological chraracterization via light and electron microscopy of the hemocytes of two cultured bivalve: a comparison study between the hard clam (Meretrix lusoria) and pacific oyster (Crassostrea gigas). Zool. Stu., 44:144-153, 2005
  13. Cima, F., Matozzo, V., Marin, M.G. and Ballarin, L.: Haemocytes of the clam Tapes philippinarum: morphofunctional characterization. Fish Shellfish Immunol., 10:677-693, 2000 https://doi.org/10.1006/fsim.2000.0282
  14. Coles, J.A. and Pipe, R.K.: Phenoloxidase activity in the haemolymph and haemocytes of the marine mussel Mytilus edulis. Fish Shellfish Immunol., 4:337-352, 1994 https://doi.org/10.1006/fsim.1994.1030
  15. Dyrynda, E.C., Wootton, E.C. and Ratcliffe, M.A.: Bivalve immunity: comparison between the marine mussel (Mytilus edulis), the edible cockle (Cerastoderma edule) and the razorshell (Ensis siliqua). Fish Shellfish Immunol., 15:195-210, 2003 https://doi.org/10.1016/S1050-4648(02)00161-4
  16. Munoz, P., Meseguer, J. and Esteban, M.A.: Phenoloxidase activity in three commercial bivalve species. Changes due to natural infestation with Perkinsus atlanticus. Fish Shellfish Immunol., 20:12-19, 2006 https://doi.org/10.1016/j.fsi.2005.02.002
  17. Feng, S.Y., Feng, J.S., Burke, C.N. and Khairallah, L.H.: Light and electron microscopy of the leucocytes of Crassostrea virginica(Mollusca: Pelecypoda). Z. Zellforsch. Mikrosk. Anat., 120:222-245, 1971 https://doi.org/10.1007/BF00335537
  18. Feng, S.Y, Feng, J.S. and Yamasu, T.: Roles of Mytilus coruscus and Crassostrea gigasblood cells in defense and nutrition. Comp. Pathol., 3:31-67, 1977
  19. Foly, D.A. and Cheng, T.C.: Interaction of mollusks and foreign substances: the morphology and behavior of hemolymph cells of the American oyster, Crassostrea virginica, in vitro. J. Invertebr. Pathol., 19:383-394, 1972 https://doi.org/10.1016/0022-2011(72)90238-8
  20. Foly, D.A. and Cheng, T.C.: A quantitative study of phagocytosis by hemolymph cells of the pelecypods Crassostrea virginica and Mercenaria mercenaria. J. Invertebr. Pathol., 25:189-197, 1975 https://doi.org/10.1016/0022-2011(75)90068-3
  21. Friedl, F.L., Alvarez, M.R., Johnson, J.S. and Gratzner, H.G.: Cytometric investigation on hemocytes of the American oyster, Crassostrea virginica. Tissue Cell, 20:933-939, 1988 https://doi.org/10.1016/0040-8166(88)90034-1
  22. Hine, P.M.: The inter-relationships of bivalve haemocytes. Fish Shellfish Immunol., 9:367-385, 1999 https://doi.org/10.1006/fsim.1998.0205
  23. Holden, J.A., Pipe, R.K., Quaglia, A. and Ciani, G.: Blood cells of the arcid clam Scarpharca inaequivalvis. J. Mar. Biol. Ass. U.K., 74:287-299, 1994 https://doi.org/10.1017/S0025315400039333
  24. Hunakoshi, S.: Studies on the classification, structure and function of hemocytes in bivalves. Bull. Natl. Res. Inst. Aqua., 29:1-103, 2000
  25. Lopez, C., Carballal, M.J., Azevedo, C. and Villalba, A.: Enzyme characterization of the circulating haemocytes of the carpet shell calm, Ruditapes decussatus (Mollusca: Bivalvia). Fish Shellfish Immunol., 7:595-608, 1997a https://doi.org/10.1006/fsim.1997.0112
  26. Lopez, C., Carballal, M. J., Azevedo, C. and Villalba, A.: Morphological characterization of the hemocytes of the clam, Ruditapes decussates (Mollusca: Bivalvia). J. Invert. Pathol., 69:51-57, 1997b https://doi.org/10.1006/jipa.1996.4639
  27. Ministry of land, transport and maritime affairs (MLTM): Statistic and annual report. Available at http://www.mltm.go.kr/USR/statistics/m_53/1st.j, 2007
  28. Nakayama, K., Nomoto, A.M., Nishijima, M. and Maruyama, T.: Morphological and functional characterization of hemocytes in the giant clam Tridacna crocea. J. Invertbr. Pathol., 69:105-111, 1997 https://doi.org/10.1006/jipa.1996.4626
  29. Narain, A.S.: The amoebocytes of lamellibranch molluscs, with special reference to the circulating amoebocytes. Malacological Rev., 6:1-12, 1973
  30. N$\ddot{e}$el, D., Bach$\acute{e}$re, E. and Mialhe. E.: Phagocytosis associated chemiluminescence of hemocytes in Mytilus edulis(Bivalvia). Dev. Comp. Immunol., 17:483-493, 1991 https://doi.org/10.1016/S0145-305X(05)80004-7
  31. Ottaviani, E., Franchini, A., Barbieri, D. and Kletsas, D.: Comparative and morphofunctional studies on Mytilus galloprovincialis haemocytes: presence of two aging-related haemocytes stages. Ital. J. Zool., 65:349-354, 1998 https://doi.org/10.1080/11250009809386772
  32. Pal, S.T. and Cajaraville, M.P.: Morphofunctional study of the haemocytes of the bivalve mollusc Mytilus galloprovincialis with emphasis on the endolysomal compartment. Cell. Struct. Funct., 20:355-367, 1995 https://doi.org/10.1247/csf.20.355
  33. Pampanin, D.M., Marin, M.G. and Ballarin, L.: Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina. Dis. Aquatic. Org., 47:227-234, 2002
  34. Park, S.I., Kwon, M.G., Cho, B.Y., Choi, H.S. and Park, M.A.: Morphological and immunological characterization of the haemocytes of the oyster, Crassostrea gigas. J. Fish. Pathol., 19:243-251, 2006. (in Korean with English abstract)
  35. Park, S.W., Lee, K.S. and Chung, E.Y.: Morphological and cytochemical characteristics of hemocytes of Meritrix lusoria (Bivalvia: Veneridae). Malacologia., 44:325-331, 2002
  36. Park, S.W, Kang, S.G. and Kim, Y.G.: The morphology of the hemocytes of the calm, Ruditapes philippinarum (Mollusca: Bivalve). J. Fish. Pathol., 13:129-136, 2000. (in Korean with English abstract)
  37. Park, S.W. and Oh, M.J.: Fish and shellfish hematology. Daekyoung. pp.151-199, 2006. (in Korean)
  38. Pipe, R.K. and Wootton, E.C.: Structural and functional characterization of the blood cells of the bivalve mullusc, Scrobicularia palna. Fish Shellfish Immunol., 15:249-262, 2003 https://doi.org/10.1016/S1050-4648(02)00164-X
  39. Renwrantz, L., Schmalmack, W., Redel, T., Friebel, B. and SchneeweiB, H.: Conversion of phenoloxidase and peroxidase indicators individual haemocytes of Mytilus edulis specimens and isolation of phenoloxidase from haemocyte extract. J. Comp. Physiol., 165(supplement B): 147-658, 1996 https://doi.org/10.1007/BF00301133
  40. Santarem, M.M., Robledo, A.F., and Figueras, A.: Seasonal change in haemocytes and serum defense factors in the blue mussel Mytilus galloprovincialis. Dis. Aquatic. Org., 18:217-222, 1994 https://doi.org/10.3354/dao018217
  41. Soderhallm, K.: Biochemical and molecular aspects of cellular communication in arthropods. Bio et Zoo., 59:141-151, 1992 https://doi.org/10.1080/11250009209386662
  42. Verghese, B., Radhakrishnan, E.V. and Padhi, A.: Effect of environmental parameters on immune response of the indian spiny lobster, Panulirus homarus (Linnaeus, 1758). Fish Shellfish Immunol., 23:928-936, 2007 https://doi.org/10.1016/j.fsi.2007.01.021
  43. Wen, C.M, Kou, G.H. and Chen, S.N.: Light and electron microscopy of hemocytes of the hard clam, Meretriz lusoria (R$\ddot{o}$ding). Comp. Biochem. Physiol., 180:279-286, 1994