Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Sequence Diversity of a Domesticated Transposase Gene, MUG1, in Oryza Species

  • Kwon, Soon-Jae (Department of Molecular Biosciences, School of Biosciences and Biotechnology, Kangwon National University) ;
  • Park, Kyong-Cheul (Department of Molecular Biosciences, School of Biosciences and Biotechnology, Kangwon National University) ;
  • Son, Jae-Han (Department of Molecular Biosciences, School of Biosciences and Biotechnology, Kangwon National University) ;
  • Bureau, Thomas (Department of Biology, McGill University) ;
  • Park, Cheul-Ho (Department of Plant Biotechnology, Kangwon National University) ;
  • Kim, Nam-Soo (Department of Molecular Biosciences, School of Biosciences and Biotechnology, Kangwon National University)
  • Received : 2009.01.06
  • Accepted : 2009.03.03
  • Published : 2009.04.30
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Abstract

MUG1 is a MULE transposon-related domesticated gene in plants. We assessed the sequence diversity, neutrality, expression, and phylogenetics of the MUG1 gene among Oryza ssp. We found MUG1 expression in all tissues analyzed, with different levels in O. sativa. There were 408 variation sites in the 3886 bp of MUG1 locus. The nucleotide diversity of the MUG1 was higher than functionally known genes in rice. The nucleotide diversity (${\pi}$) in the domains was lower than the average nucleotide diversity in whole coding region. The ${\pi}$ values in nonsynonymous sites were lower than those of synonymous sites. Tajima D and Fu and Li $D^*$ values were mostly negative values, suggesting purifying selection in MUG1 sequences of Oryza ssp. Genome-specific variation and phylogenetic analyses show a general grouping of MUG1 sequences congruent with Oryza ssp. biogeography; however, our MUG1 phylogenetic results, in combination with separate B and D genome studies, might suggest an early divergence of the Oryza ssp. by continental drift of Gondwanaland. O. long-istaminata MUG1 divergence from other AA diploids suggests that it might not be a direct ancestor of the African rice species.

Keywords

Acknowledgement

Supported by : Korean Research Foundation, Ministry of Education of Korea

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