Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antifungal Activity against Trichoderma spp. of Water Soluble Essential Oil extracted from Pinus densiflora and Chamaecyparis obtusa

소나무 및 편백나무 수용성 정유를 이용한 Trichoderma spp.의 생장억제 활성

  • Yeo, Hee Dong (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Jung, Ji-Young (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Nam, Jung Bin (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Ji Woon (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Hee Kyu (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Choi, Myung Suk (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Alm, Glen (Department of Plant Agriculture, University of Guelph) ;
  • Rinker, Danny Lee (Department of Plant Agriculture, University of Guelph) ;
  • Yang, Jae-Kyung (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University)
  • 여희동 (경상대학교 환경산림과학부, 농업생명과학연구원) ;
  • 정지영 (경상대학교 환경산림과학부, 농업생명과학연구원) ;
  • 남정빈 (경상대학교 환경산림과학부, 농업생명과학연구원) ;
  • 김지운 (경상대학교 환경산림과학부, 농업생명과학연구원) ;
  • 김희규 (경상대학교 응용생물환경학전공) ;
  • 최명석 (경상대학교 환경산림과학부, 농업생명과학연구원) ;
  • ;
  • ;
  • 양재경 (경상대학교 환경산림과학부, 농업생명과학연구원)
  • Received : 2009.05.21
  • Accepted : 2009.09.30
  • Published : 2009.11.25
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Abstract

This study was carried out to investigate the antifungal activity of the water soluble essential oil against Trichoderma spp. Water soluble essential oils from Pinus densiflora and Chamaecyparis obtusa were obtained from GAP (Gas assisted process) extraction apparatus. When the water soluble essential oil was treated, the growth of the conidial germination was maximum under the cultivation condition at $25^{\circ}C$ and in the culture medium adjusted to pH 5.0. The yield of water soluble essential oil was 3.9% and 3.7% in P. densiflora and C. obtusa, respectively. 24 and 15 kinds of compounds were identified in water soluble essential oils of P. densiflora and C. obtusa, respectively. The major components in the essential oil of P. densiflora were $\alpha$-Terpineol acetate and Terpinen-4-ol and those of C. obtusa were Terpinen-4-ol and $\alpha$-Terpineol. Antifungal activity was the best in the 5000 ppm of P. densiflora against Trichoderma harzianum and in 5000 ppm of C. obtusa against Trichoderma atroviride.

본 연구는 수목으로부터 분리된 수용성 정유를 이용하여 버섯에 병해를 일으키는 푸른곰팡이병의 원인 미생물인 Trichoderma에 대한 항균활성을 확인하고자 시도되었다. 소나무(Pinus densiflora)와 편백나무(Chamaecyparis obtusa) 잎으로부터 GAP (Gas assisted process)을 이용하여 수용성 정유를 획득하였다. 버섯의 푸른 곰팡이병을 발생시키는 Trichoderma spp. 곰팡이 5종은 $25^{\circ}C$에서 균사생장이 가장 높았으며, pH 5.0의 배지조건에서 가장 양호한 균사 생장을 나타내었다. 소나무와 편백나무 잎의 수용성 정유는 3.9% 및 3.7%의 수율을 나타내었다. 소나무와 편백나무 잎 정유의 화학적 조성은 동일 화합물이 많았으며, 동일 화합물로는 $\alpha$-Terpineol acetate, Terpinen-4-ol 및 $\alpha$-Terpineol이 확인되었다. 소나무 잎의 수용성 정유는 5000 ppm 농도에서 Trichoderma harzianum에 대하여 가장 높은 항균활성을 나타냈으며, 편백나무 잎의 수용성 정유 또한 5000 ppm 농도에서 Trichoderma atroviride에 대하여 가장 높은 항균활성을 나타내었다.

Keywords

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