BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Chemopreventive effects of polysaccharides extract from Asterina pectinifera on HT-29 human colon adenocarcinoma cells

  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Shon, Yun-Hee (Intractable Disease Research Center, Dongguk University)
  • Published : 2009.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

We examined the effects of polysaccharides extracted from Asterina pectinifera on the activities of quinone reductase (QR), glutathione S-transferase (GST), ornithine decarboxylase (ODC), cyclooxygenase (COX)-2 and glutathione (GSH) levels in HT-29 human colon adenocarcinoma cells. We found that the polysaccharides extract induced QR activity in a dose-dependent manner over a concentration range of $20-60\;{\mu}g/ml$ and increased GST activity as much as 1.4-fold over controls. GSH levels were increased 1.3- and 1.5-fold with the extract at 40 and $60\;{\mu}g/ml$, respectively. The activity and protein expression of ODC in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced colon cancer cells was inhibited by the extract. The polysaccharides suppressed TPA-induced prostaglandin (PG) production. These data indicate that polysaccharides from A. pectinifera increase phase II detoxification enzyme activity and inhibit ODC and COX-2 activities in HT-29 human colon adenocarcinoma cells. Consequently, this effect may contribute to the protective effect of polysaccharides from A. pectinifera against colon cancer.

Keywords

References

  1. Parkin, D. M., Pisani, P. and Ferlay, J. (1993) Estimates of the worldwide incidence of eighteen major cancers in 1985. Int. J. Cancer 54, 594-606 https://doi.org/10.1002/ijc.2910540413
  2. Talalay, P., Fahey, J. W., Holtzclaw, W. D., Prestera, T. and Zhang, Y. (1995) Chemoprotection against cancer by phase II enzyme induction. Toxicol. Lett. (Amst.). 82, 173-179 https://doi.org/10.1016/0378-4274(95)03553-2
  3. Talalay, P., DeLong, M. J. and Prochaska, H. J. (1987) Molecular mechanisms in protection against carcinogenesis; in Cancer Biology and Therapeutics, Cory, J. G. and Szentivanyi, A. (eds), 197-216, Plenum, New York, USA
  4. Chasseaud, L. F. (1979) The role of glutathione and glutathione S-transferases in the metabolism of chemical carcinogens and other electrophilic agents. Adv. Cancer Res. 29, 176-274
  5. Cho, H. Y., Lee, H. J. and Kong, K. H. (2007) A phi class glutathione S transferase from Oryza sativa (OsGSTF5): molecular cloning, expression and biochemical characteristics. J. Biochem. Mol. Biol. 40, 511-516 https://doi.org/10.5483/BMBRep.2007.40.4.511
  6. Tanako, T., Kojima, T., Suzuki, M. and Mori, H. (1993) Chemoprevention of colon carcinogenesis by the natural product of a simple phenolic compound protocatechuic acid: suppressing effects on tumor development and biomarkers expression of colon. Cancer Res. 53, 3908-3913
  7. An, J. J., Kim, S. Y., Lee, S. H., Kim, D. W., Ryu, H. J., Yeo, S. I., Jang, S. H., Kwon, H. J., Kim, T. Y., Lee, S. C., Poo, H., Cho, S. W., Lee, K. S., Park, J., Eum, W. S. and Choi, S. Y. (2007) Transduced PEP-1-Grb7 fusion protein suppressed LPS-induced COX-2 expression. J. Biochem. Mol. Biol. 40, 189-195 https://doi.org/10.5483/BMBRep.2007.40.2.189
  8. Eberhart, C. E., Coffey, R. J., Radhika, A., Giardiello, F. M., Ferrenbach, S. and DuBois, R. N. (1994) Up-regulation of cyclooxygenase -2 gene expression in human colorectal adenomas and adenocarcinomas. Gastroenterology 107, 1183-1188 https://doi.org/10.1016/0016-5085(94)90246-1
  9. Marnett, L. J. and DuBois, R. N. (2002) COX-2: a target for colon cancer prevention. Annu. Rev. Pharmacol. Toxicol. 42, 55-80 https://doi.org/10.1146/annurev.pharmtox.42.082301.164620
  10. Cragg, G. M., Newman, D. J. and Weiss, R. B. (1997) Coral reefs, forests, and thermal vent: the worldwide exploration of nature for novel antitumor agents. Semin. Ocol. 24, 156-163
  11. Hecht, S. S. (1995) Chemoprevention by isothiocyanates. J. Cell. Biochem. 22, 195-209
  12. Shon, Y. H. and Nam, K. S. (2001) Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. J. Ethnopharmacol. 77, 103-109 https://doi.org/10.1016/S0378-8741(01)00276-8
  13. Marton, L. J. and Pegg, A. E. (1995) Polyamines as targets for therapeutic intervention. Annu. Rev. Pharmacol. Toxicol. 35, 55-91 https://doi.org/10.1146/annurev.pa.35.040195.000415
  14. Rao, C. V., Simi, B. and Reddy, B. S. (1993) Inhibition by dietary curcumin of azoxymethane-induced ornithine decarboxylase, tyrosine protein kinase, arachidonic acid metabolism and aberrant crypt foci formation in the rat colon. Carcinogenesis 14, 2219-2225 https://doi.org/10.1093/carcin/14.11.2219
  15. Kawamori, T., Rao, C. V., Seibert, K. and Reddy, B. S. (1998) Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis. Cancer Res. 58, 409-412
  16. Shon, Y. H. and Nam, K. S. (2002) Cancer chemoprevention: inhibitory effect of soybeans fermented with basidiomycetes on 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol- 13-acetate-induced mouse skin carcinogenesis. Biotechnol. Lett. 24, 1005-1010 https://doi.org/10.1023/A:1015673229528
  17. Shon, Y. H., Nam, K. S. and Kim, M. K. (2004) Cancer chemopreventive potential of Scenedesmus spp. cultured in medium containing bioreacted swine urine. J. Microbiol. Biotechnol. 14, 158-161
  18. Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974) Glutathione S-transferases : the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139
  19. Richie, J. P. Jr., Showronski, L., Abraham, P. and Leutzinger, Y. (1996) Blood glutathione concentrations in a large-scale human study. Clin. Chem. 42, 64-70
  20. Shon, Y. H. and Nam, K. S. (2003) Inhibition of polyamine biosynthesis of Acanthamoeba castellanii and 12-O-tetradecanoylphorbol- 13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide. Biotechnol. Lett. 25, 701-704 https://doi.org/10.1023/A:1023480701270

Cited by

  1. Starase: A bi-functional fibrinolytic protease from hepatic caeca of Asterina pectinifera displays antithrombotic potential vol.105, 2014, https://doi.org/10.1016/j.biochi.2014.06.012
  2. Inhibitory effect of starfish polysaccharides on metastasis in HT-29 human colorectal adenocarcinoma vol.17, pp.4, 2012, https://doi.org/10.1007/s12257-012-0099-x
  3. Effects of C-Phycocyanin on the representative genes of tumor development in mouse skin exposed to 12-O-tetradecanoyl-phorbol-13-acetate vol.34, pp.3, 2012, https://doi.org/10.1016/j.etap.2012.08.001
  4. Bean (Phaseolus vulgaris L.) polysaccharides modulate gene expression in human colon cancer cells (HT-29) vol.43, pp.4, 2010, https://doi.org/10.1016/j.foodres.2010.01.017
  5. Poly-γ-Glutamic Acid Induces Apoptosis via Reduction of COX-2 Expression in TPA-Induced HT-29 Human Colorectal Cancer Cells vol.16, pp.4, 2015, https://doi.org/10.3390/ijms16047577
  6. Antioxidant and Antimicrobial Activity of Ophiurasaponin Extracted fromOphiopholis mirabilis vol.2014, 2014, https://doi.org/10.1155/2014/646537
  7. Preparation, preliminary characterization and inhibitory effect on human colon cancer HT-29 cells of an acidic polysaccharide fraction from Stachys floridana Schuttl. ex Benth vol.60, 2013, https://doi.org/10.1016/j.fct.2013.07.060
  8. Anti-inflammatory effect of the extract from fermented Asterina pectinifera with Cordyceps militaris mycelia in LPS-induced RAW264.7 macrophages vol.26, pp.6, 2017, https://doi.org/10.1007/s10068-017-0233-9