Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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The Arabidopsis AtLEC Gene Encoding a Lectin-like Protein Is Up-Regulated by Multiple Stimuli Including Developmental Signal, Wounding, Jasmonate, Ethylene, and Chitin Elicitor

  • Lyou, Seoung Hyun (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Park, Hyon Jin (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Jung, Choonkyun (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Sohn, Hwang Bae (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Garam (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Kim, Chung Ho (Department of Food and Nutrition, Seowon University) ;
  • Kim, Minkyun (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Choi, Yang Do (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Cheong, Jong-Joo (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University)
  • Received : 2008.09.04
  • Accepted : 2008.10.13
  • Published : 2009.01.31
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Abstract

The Arabidopsis gene AtLEC (At3g15356) gene encodes a putative 30-kDa protein with a legume lectin-like domain. Likely to classic legume lectin family of genes, AtLEC is expressed in rosette leaves, primary inflorescences, and roots, as observed in Northern blot analysis. The accumulation of AtLEC transcript is induced very rapidly, within 30 min, by chitin, a fungal wall-derived oligosaccharide elictor of the plant defense response. Transgenic Arabidopsis carrying an AtLEC promoter-driven ${\beta}$-glucuronidase (GUS) construct exhibited GUS activity in the leaf veins, secondary inflorescences, carpel heads, and silique receptacles, in which no expression could be seen in Northern blot analysis. This observation suggests that AtLEC expression is induced transiently and locally during developmental processes in the absence of an external signal such as chitin. In addition, mechanically wounded sites showed strong GUS activity, indicating that the AtLEC promoter responds to jasmonate. Indeed, methyl jasmonate and ethylene exposure induced AtLEC expression within 3-6 h. Thus, the gene appears to play a role in the jasmonate-/ethylene-responsive, in addition to the chitin-elicited, defense responses. However, chitin-induced AtLEC expression was also observed in jasmonate-insensitive (coi1) and ethylene-insensitive (etr1-1) Arabidopsis mutants. Thus, it appears that chitin promotes AtLEC expression via a jasmonate- and/or ethylene-independent pathway.

Keywords

Acknowledgement

Supported by : Crop Functional Genomics Center, Korea Ministry of Science and Technology, Ministy of Euducation, Korea Research Foundation

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