Asian-Australasian Journal of Animal Sciences

  • 제22권4호
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  • Pages.500-506
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  • 2009
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium

  • Park, Kwang-Wook (The Graduate School of Natural Science and Technology, Faculty of Agriculture, Okayama University) ;
  • Niwa, Koji (The Graduate School of Natural Science and Technology, Faculty of Agriculture, Okayama University)
  • 투고 : 2008.08.04
  • 심사 : 2008.10.14
  • 발행 : 2009.04.01
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초록

A modified Tris-buffered medium (mTBM) has been widely used as an insemination medium for porcine in vitro fertilization (IVF). We examined whether mTBM could be used for bovine IVF. Bovine cumulus-oocyte complexes (COCs) were cultured in a serum-free medium containing 30 ng/ml EGF for 22 h. After culture, COCs were inseminated with spermatozoa for 12 h in mTBM containing 5 mM caffeine and 10 g/ml heparin. The penetration of oocytes increased significantly (p<0.05) as the sperm concentration increased from 0.1 (30%) to 1-10 $(87-100%){\times}10^6$ cells/ml. This was significantly different from values obtained at 1 (87%) and 10 $(100%){\times}10^6$ cells/ml. However, when COCs were inseminated with spermatozoa from different bulls, the proportions (62-100%) of oocytes penetrated varied according to the bull. The proportion (18%) of oocytes penetrated was significantly (p<0.05) lower in a fertilization medium without caffeine and heparin but increased with the addition of caffeine and/or heparin to the medium, and the proportion (93-96%) of oocytes penetrated increased significantly (p<0.05) when the medium was supplemented with heparin and caffeine. In this medium, sperm penetration was first observed at 3 h after insemination. Irrespective of the presence of glucose in the fertilization medium, the proportion (93-97%) of oocytes penetrated and the proportion (83-84%) of embryos at the ${\geq}2$-cell stage cultured in a chemically defined medium were not significantly different. However, the proportion of embryos developing to the blastocyst stage was significantly (p<0.05) higher in the presence (11%) of glucose in the fertilization medium than in its absence (2%). In conclusion, the present study demonstrated that bovine oocytes penetrated in vitro in mTBM can develop to the blastocyst stage and mTBM may be used for the in vitro production of bovine embryos.

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참고문헌

  1. Abeydeera, L. R. and B. N. Day. 1997a. In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. Theriogenology 48:537-544 https://doi.org/10.1016/S0093-691X(97)00270-7
  2. Abeydeera, L. R., B. and N. Day. 1997b. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734 https://doi.org/10.1095/biolreprod57.4.729
  3. Abeydeera, L. R., H. Funahashi, N.-H. Kim and B. N. Day. 1997. Chlortetracycline fluorescence patterns and in vitro fertilization of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations. Zygote 5:117-125
  4. Abeydeera, L. R., W. H. Wang, R. S. Prather and B. N. Day. 1998. Maturation in vitro of pig oocytes in protein-free media Fertilization and subsequent embryo development in vitro. Biol. Reprod. 58:1316-1320 https://doi.org/10.1095/biolreprod58.5.1316
  5. Berger, T. and M. B. Horton. 1988. Evaluation of assay conditions for the zona-free hamster ova bioassay of boar sperm fertility. Gamate Res. 19:101-111 https://doi.org/10.1002/mrd.1120190110
  6. Bhattacharyya, A. and R. Yanagimachi. 1988. Synthetic organic pH buffers can support fertilization of guinea pig eggs, but not as efficiently as bicarbonate buffer. Gamete Res. 19:123-129 https://doi.org/10.1002/mrd.1120190203
  7. Clarke, R. N. and L. A. Johnson. 1987. Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro. Gamete Res. 16:193-204 https://doi.org/10.1002/mrd.1120160302
  8. First, N. L. and J. J. Parrish. 1988. Sperm maturation and in vitro fertilization. Proc. 11th Intern. Congr. Anim. Reprod. AI. 5:160-168
  9. Fraser, L. R. 1979. Accelerated mouse sperm penetration in vitro in the presence of caffeine. J. Reprod. Fertil. 57:377-384 https://doi.org/10.1530/jrf.0.0570377
  10. Fraser, L. R., L. R. Abeydeera and K. Niwa. 1995. Ca2+- regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol. Reprod. Dev. 40:233-241 https://doi.org/10.1002/mrd.1080400213
  11. Fraser, L. R. and P. J. Quinn. 1981. A glycolitic product is obligatory for initiation of the sperm acrosome reaction and whiplash motility required for fertilization in the mouse. J. Reprod. Fertil. 61:25-35 https://doi.org/10.1530/jrf.0.0610025
  12. Garbers, D. L., N. L. First, S. K. Gorman and H. A. Lardy. 1971. Stimulation and maintenance of ejaculated bovine spermatozoa respiration and motility by caffeine. Biol. Reprod. 5:336-339
  13. Garbers, D. L., N. L. First, S. K. Gorman and H. A. Lardy. 1973. The effects of cyclic nucleotide phosphodiesterase inhibitors on ejaculated porcine spermatozoa metabolism. Biol. Reprod. 8:599-606 https://doi.org/10.1016/S0093-691X(97)00270-7
  14. Handrow, R. R., N. L. First and J. J. Parrish. 1989. Calcium requirement and increased associated with bovine sperm during capacitation by heparin. J. Exp. Zool. 252:174-182 https://doi.org/10.1002/jez.1402520209
  15. Handrow, R. R., R. W. Lenz and R. L. Ax. 1982. Structural comparisons among glycosaminoglycans to promote an acrosome reaction in bovine spermatozoa. Biochem. Biophys. Res. Commun. 107:1326-1332 https://doi.org/10.1016/S0006-291X(82)80143-5
  16. Hansen, P. J. 2005. Realizing the promise of IVF in cattle. Theriogenology 65:119-125 https://doi.org/10.1016/j.theriogenology.2005.09.019
  17. Hyne, R. V. and K. P. Edwards. 1985. Influence of 2-deoxy-Dglucose and energy substrates on guinea-pig sperm capacitation and acrosome reaction. J. Reprod. Fertil. 73:59-69 https://doi.org/10.1016/S0093-691X(98)00026-0
  18. Iritani, A. and K. Niwa. 1977. Capacitation of bull spermatozoa and fertilization in vitro of cattle follicular oocytes matured in culture. J. Reprod. Fertil. 50:119-121 https://doi.org/10.1530/jrf.0.0500119
  19. Jang, H. Y., Y. S. Jung, H. T. Cheong, J. T. Kim, C. K. Park, H. S. Kong, H. K. Lee and B. K. Yang. 2008. Effects of cell status of Bovine Oviduct Epithelial Cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust. J. Anim. Sci. 21:980-987
  20. Kusunoki, H., M. Sakaue, S. Kato and K. Sunao. 1989. Induction of the acrosome reaction in ejaculated goat spermatozoa by preincubation in chemically defined medium. J. Exp. Zool. 249:322-328 https://doi.org/10.1002/jez.1402490311
  21. Lee, M. A. and B. D. Storey. 1986. Bicarbonate is essential for fertilization of mouse eggs: mouse sperm required to undergo the acrosome reaction. Biol. Reprod. 34:349-356 https://doi.org/10.1095/biolreprod34.2.349
  22. Lim, J. M., J. H. Kim, K. Okuda and K. Niwa. 1993. Effect of the presence of glucose during fertilization and/or culture in a chemically semi-defined medium on the development of bovine oocytes matured and fertilized in vitro. J. Reprod. Dev. 39:237-242 https://doi.org/10.1262/jrd.39.237
  23. Neill, J. M. and P. Olds-Clarke. 1987. A computer-assisted assay for mouse sperm hyperactivation demonstrates that bicarbonate but not bovine serum albumin is required. Gamete Res. 18:121-140 https://doi.org/10.1002/mrd.1120180204
  24. Niwa, K. and O. Ohgoda. 1988. Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology 30:733-741 https://doi.org/10.1016/0093-691X(88)90308-1
  25. Park, C. K., O. Ohgoda and K. Niwa. 1989. Penetration of bovine follicular oocytes by frozen-thawed spermatozoa in the presence of caffeine and heparin. J. Reprod. Fertil. 86:577-582 https://doi.org/10.1530/jrf.0.0860577
  26. Park, K. W., K. Iga and K. Niwa. 1997. Exposure of bovine oocytes to EGF during maturation allows them to development to blastocysts in a chemically-defined medium. Theriogenology 48:1127-1135 https://doi.org/10.1016/S0093-691X(97)00345-2
  27. Parrish, J. J., J. L. Susko-Parrish and N. L. First. 1985. Effect of heparin and chondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro. Theriogenology 24:537-549 https://doi.org/10.1016/0093-691X(85)90060-3
  28. Parrish, J. J., J. L. Susko-Parrish and N. L. First. 1989. Capacitation of bovine sperm by heparin: inhibitory effect of glucose and role of intracellular pH. Biol. Reprod. 41:683-699 https://doi.org/10.1095/biolreprod41.4.683
  29. Shioya, Y., M. Kuwayama, M. Fukushima, S. Iwasaki and A. Hanada. 1988. In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro. Theriogenology 30:489-496 https://doi.org/10.1016/0093-691X(88)90198-7
  30. Suzuki, K., M. Ebihara, T. Nagai, N. G. E. Clarke and R. A. P. Harrison. 1994. Importance of bicarbonate/$CO_{2}$ for fertilization of pig oocytes in vitro, and synergism with caffeine. Reprod. Fertil. Dev. 6:221-227 https://doi.org/10.1071/RD9940221
  31. Tajik, P. and K. Niwa. 1998. Effects of caffeine and/or heparin in a chemically defined medium with or without glucose on in vitro penetration of bovine oocytes and their subsequent development. Theriogenology 49:771-777 https://doi.org/10.1016/S0093-691X(98)00026-0
  32. Tajik, P., K. Niwa and T. Murase. 1993. Effects of different protein supplements in fertilization medium on in vitro penetration of cumulus-intact and cumulus-free bovine oocytes matured in culture. Theriogenology 40:949-958 https://doi.org/10.1016/0093-691X(93)90363-A
  33. Tajik, P., W. H. Wang, K. Okuda and K. Niwa. 1994. In vitro fertilization of bovine oocytes in a chemically defined, proteinfree medium varying the bicarbonate concentration. Biol. Reprod. 50:1231-1237 https://doi.org/10.1095/biolreprod50.6.1231
  34. Tsuzuki, D., H. Duran, M. Sawamizu, K. Ashizawa and N. Fujihara. 2000. The effects of dimethyl-sulfoxide added to the fertilization medium on the motility and the acrosome reaction of spermatozoa and the subsequent development of oocytes in Bovine. Asian-Aust. J. Anim. Sci. 13:739-747
  35. Wang, W. H., L. R. Abeydeer, L. R. Fraser and K. Niwa. 1995. Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen-thawed ejaculated boar spermatozoa incubated in a protein-free chemically defined medium. J. Reprod. Fertil. 104:305-313 https://doi.org/10.1530/jrf.0.1040305
  36. Wang, W. H., K. Niwa and K. Okuda. 1991. In-vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil. 93:491-496 https://doi.org/10.1016/S0093-691X(97)00034-4
  37. Xu, K. P. and T. Greve. 1988. A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes. J. Reprod. Fertil. 82:127-134 https://doi.org/10.1530/jrf.0.0820127