Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Functional Characteristics of TRPC4 Channels Expressed in HEK 293 Cells

  • Sung, Tae Sik (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Kim, Min Ji (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Hong, Soojin (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Jeon, Jae-Pyo (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Kim, Byung Joo (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Jeon, Ju-Hong (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine) ;
  • Kim, Seon Jeong (Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University) ;
  • So, Insuk (Center for Bio-Artificial Muscle and Department of Physiology, Seoul National University College of Medicine)
  • Received : 2008.08.12
  • Accepted : 2008.12.01
  • Published : 2009.02.28
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Abstract

The classical type of transient receptor potential (TRPC) channel is a molecular candidate for $Ca^{2+}$-permeable cation channels in mammalian cells. Because TRPC4 and TRPC5 belong to the same subfamily of TRPC, they have been assumed to have the same physiological properties. However, we found that TRPC4 had its own functional characteristics different from those of TRPC5. TRPC4 channels had no constitutive activity and were activated by muscarinic stimulation only when a muscarinic receptor was co-expressed with TRPC4 in human embryonic kidney (HEK) cells. Endogenous muscarinic receptor appeared not to interact with TRPC4. TPRC4 activation by $GTP{\gamma}S$ was not desensitized. TPRC4 activation by $GTP{\gamma}S$ was not inhibited by either Rho kinase inhibitor or MLCK inhibitor. TRPC4 was sensitive to external pH with $pK_a$ of 7.3. Finally, TPRC4 activation by $GTP{\gamma}S$ was inhibited by the calmodulin inhibitor W-7. We conclude that TRPC4 and TRPC5 have different properties and their own physiological roles.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation

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