한국어병학회지 (Journal of fish pathology)

  • 제22권2호
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  • Pages.173-177
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  • 2009
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Comparison of lymphocystis disease virus proteins between marine and freshwater fish

  • Hossain, Mosharrof (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kim, Wi-Sik (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kim, Seok-Ryel (Pathology division, National Fisheries Research and Development Institute) ;
  • Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
  • 발행 : 2009.08.01
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초록

Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.

키워드

참고문헌

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