Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Functional Analysis of the Stress-Inducible Soybean Calmodulin Isoform-4 (GmCaM-4) Promoter in Transgenic Tobacco Plants

  • Park, Hyeong Cheol (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kim, Man Lyang (Department of Infection Biology, Biozentrum, University of Basel) ;
  • Kang, Yun Hwan (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Jeong, Jae Cheol (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Cheong, Mi Sun (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Choi, Wonkyun (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Lee, Sang Yeol (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Cho, Moo Je (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kim, Min Chul (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Chung, Woo Sik (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Yun, Dae-Jin (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
  • Received : 2009.01.22
  • Accepted : 2009.02.26
  • Published : 2009.04.30
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Abstract

The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.

Keywords

Acknowledgement

Supported by : Rural Development Administration, Ministry of Education, Science and Technology in Korea

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