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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Kv1.3 voltage-gated K+ channel subunit as a potential diagnostic marker and therapeutic target for breast cancer

  • Jang, Soo-Hwa (Laboratories of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ryu, Pan-Dong (Laboratories of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, So-Yeong (Laboratories of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University)
  • Published : 2009.08.31
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Abstract

Voltage-gated $K^+$ (Kv) channels are widely expressed in the plasma membranes of numerous cells such as epithelial cells. Recently, it has been demonstrated that Kv channels are associated with the proliferation of several types of cancer cells. Specifically, Kv1.3 seems to be involved in cancer cell proliferation and apoptosis. In the present study, we examined the expression of Kv1.3 in immortalized and tumorigenic human mammary epithelial cells. We also evaluated the expression level of Kv1.3 in each stage of breast cancer using mRNA isolated from breast cancer patients. In addition, treatment with tetraethylammonium, a Kv channel blocker, suppressed tumorigenic human mammary epithelial cell proliferation. Therefore, Kv1.3 may serve as a novel molecular target for breast cancer therapy while its stage-specific expression pattern may provide a potential diagnostic marker for breast cancer development.

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References

  1. Ouadid-Ahidouch, H., Chaussade, F., Roudbaraki, M., Slomianny, C., Dewailly, E., Delcourt, P. and Prevarskaya, N. (2000) KV1.1 K(+) channels identification in human breast carcinoma cells: involvement in cell proliferation. Biochem. Biophys. Res. Commun. 278, 272-277 https://doi.org/10.1006/bbrc.2000.3790
  2. Abdul, M., Santo, A. and Hoosein, N. (2003) Activity of potassium channel-blockers in breast cancer. Anticancer Res. 23, 3347-3351
  3. Lan, M., Shi, Y., Han, Z., Hao, Z., Pan, Y., Liu, N., Guo, C., Hong, L., Wang, J., Qiao, T. and Fan, D. (2005) Expression of delayed rectifier potassium channels and their possible roles in proliferation of human gastric cancer cells. Cancer Biol. Ther. 4, 1342-1347 https://doi.org/10.4161/cbt.4.12.2175
  4. Abdul, M. and Hoosein, N. (2002) Expression and activity of potassium ion channels in human prostate cancer. Cancer Lett. 186, 99-105 https://doi.org/10.1016/S0304-3835(02)00348-8
  5. Abdul, M. and Hoosein, N. (2006) Reduced Kv1.3 potassium channel expression in human prostate cancer. J. Membr. Biol. 214, 99-102 https://doi.org/10.1007/s00232-006-0065-7
  6. Szabo, I., Adams, C. and Gulbins, E. (2004) Ion channels and membrane rafts in apoptosis. Pflugers. Arch. 448, 304-312 https://doi.org/10.1007/s00424-004-1259-4
  7. Brevet, M., Ahidouch, A., Sevestre, H., Merviel, P., El Hiani, Y., Robbe, M. and Ouadid-Ahidouch, H. (2008) Expression of K+ channels in normal and cancerous human breast. Histol. Histopathol. 23, 965-972
  8. Kang, K. S., Sun, W., Nomata, K., Morita, I., Cruz, A., Liu, C. J., Trosko, J. E. and Chang, C. C. (1998) Involvement of tyrosine phosphorylation of p185 (c-erbB2/neu) in tumorigenicity induced by X-rays and the neu oncogene in human breast epithelial cells. Mol. Carcinog 21, 225-233 https://doi.org/10.1002/(SICI)1098-2744(199804)21:4<225::AID-MC1>3.0.CO;2-J
  9. Ohya, S., Kimura, K., Niwa, S., Ohno, A., Kojima, Y., Sasaki, S., Kohri, K. and Imaizumi, Y. (2009) Malignancy grade-dependent expression of K(+)-channel subtypes in human prostate cancer. J. Pharmacol. Sci. 109, 148-151 https://doi.org/10.1254/jphs.08208SC
  10. Wood, L. D., Parsons, D. W., Jones, S., Lin, J., Sjoblom, T., Leary, R. J., Shen, D., Boca, S. M., Barber, T., Ptak, J., Silliman, N., Szabo, S., Dezso, Z., Ustyanksky, V., Nikolskaya, T., Nikolsky, Y., Karchin, R., Wilson, P. A., Kaminker, J. S., Zhang, Z., Croshaw, R., Willis, J., Dawson, D., Shipitsin, M., Willson, J. K., Sukumar, S., Polyak, K., Park, B. H., Pethiyagoda, C. L., Pant, P. V., Ballinger, D. G., Sparks, A. B., Hartigan, J., Smith, D. R., Suh, E., Papadopoulos, N., Buckhaults, P., Markowitz, S. D., Parmigiani, G., Kinzler, K. W., Velculescu, V. E. and Vogelstein, B. (2007) The genomic landscapes of human breast and colorectal cancers. Science 318, 1108-1113 https://doi.org/10.1126/science.1145720
  11. Harris, L., Fritsche, H., Mennel, R., Norton, L., Ravdin, P., Taube, S., Somerfield, M. R., Hayes, D. F. and Bast, R. C., Jr. (2007) American Society of Clinical Oncology 2007 update of recommendations for the use of tumor markers in breast cancer. J. Clin. Oncol. 25, 5287-5312 https://doi.org/10.1200/JCO.2007.14.2364
  12. Andersson, I. and Ryden, S. (2001) Early detection and prevention: benefits, costs and limitations of screening; In: Tobias, J. S., Houghton, J., Henderson, I. C. (eds.), pp. 105- 117, Breast cancer, Arnold, London
  13. Nie, L., Wu, G. and Zhang, W. (2006) Correlation of mRNA expression and protein abundance affected by multiple sequence features related to translational efficiency in Desulfovibrio vulgaris: a quantitative analysis. Genetics 174, 2229-2243 https://doi.org/10.1534/genetics.106.065862
  14. Chen, G., Gharib, T. G., Huang, C. C., Taylor, J. M., Misek, D. E., Kardia, S. L., Giordano, T. J., Iannettoni, M. D., Orringer, M. B., Hanash, S. M. and Beer, D. G. (2002) Discordant protein and mRNA expression in lung adenocarcinomas. Mol. Cell Proteomics 1, 304-313 https://doi.org/10.1074/mcp.M200008-MCP200
  15. Spitzner, M., Ousingsawat, J., Scheidt, K., Kunzelmann, K. and Schreiber, R. (2007) Voltage-gated K+ channels support proliferation of colonic carcinoma cells. Faseb. J. 21, 35-44 https://doi.org/10.1096/fj.06-6200com
  16. Shon, Y. H., Park, S. D. and Nam, K. S. (2006) Effective chemopreventive activity of genistein against human breast cancer cells. BMB Rep. 39, 448-451 https://doi.org/10.5483/BMBRep.2006.39.4.448
  17. Eling, T. E., Baek, S. J., Shim, M. S. and Lee, C. H. (2006) NSAID activated gene (NAG-1), as modulator of tumorigenesis. BMB Rep. 39, 649-655 https://doi.org/10.5483/BMBRep.2006.39.6.649
  18. Kao, C. Y., Nomata, K., Oakley, C. S., Welsch, C. W. and Chang, C. C. (1995) Two types of normal human breast epithelial cells derived from reduction mammoplasty: phenotypic characterization and response to SV40 transfection. Carcinogenesis 16, 531-538 https://doi.org/10.1093/carcin/16.3.531

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