Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows

  • Yang, Yong-xin (College of Veterinary Medicine, Gansu Agricultural University) ;
  • Cao, Sui-zhong (College of Animal Science and Technology, Sichuan Agriculture University) ;
  • Zhang, Yong (College of Veterinary Medicine, Gansu Agricultural University) ;
  • Zhao, Xing-xu (College of Veterinary Medicine, Gansu Agricultural University)
  • Received : 2008.08.26
  • Accepted : 2009.01.23
  • Published : 2009.06.01
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Abstract

To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.

Keywords

References

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