Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Cancer-Upregulated Gene 2 (CUG2), a New Component of Centromere Complex, Is Required for Kinetochore Function

  • Kim, Hyejin (Department of Microbiology, Chungnam National University) ;
  • Lee, Miae (Department of Microbiology, Chungnam National University) ;
  • Lee, Sunhee (Department of Microbiology, Chungnam National University) ;
  • Park, Byoungwoo (Department of Microbiology, Chungnam National University) ;
  • Koh, Wansoo (Department of Microbiology, Chungnam National University) ;
  • Lee, Dong Jun (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lim, Dae-Sik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lee, Soojin (Department of Microbiology, Chungnam National University)
  • Received : 2009.04.14
  • Accepted : 2009.04.22
  • Published : 2009.06.30
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Abstract

We previously identified cancer-upregulated gene 2 (CUG2) as a commonly up-regulated gene in various human cancer tissues, especially in ovary, liver, and lung (Lee et al., 2007a). CUG2 was determined to be a nuclear protein that exhibited high proto-oncogenic activities when overexpressed in NIH3T3 mouse fibroblast cells. To identify other cellular functions of CUG2, we performed yeast two-hybrid screening and identified CENP-T, a component of CENP-A nucleosome complex in the centromere, as an interacting partner of CUG2. Moreover, CENP-A, the principle centromeric determinant, was also found in complex with CENP-T/CUG2. Immunofluorescent staining revealed the co-localization of CUG2 with human centromeric markers. Inhibition of CUG2 expression drastically affected cell viability by inducing aberrant cell division. We propose that CUG2 is a new component of the human centromeric complex that is required for proper chromosome segregation during mitosis.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology of Korea, Korea Research Foundation

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