Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Identification of a Natural Hybrid between the Striped Spine Loach Cobitis tetralineata and the King Spine Loach Iksookimia longicorpa by Analyzing Mitochondrial COI and Nuclear RAG1 Sequences

미토콘드리아 COI와 핵 RAG1 유전자 분석에 의한 줄종개(Cobitis tetralineata)와 왕종개(Iksookimia longicorpa) 간 자연잡종 동정

  • Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Yang, Hyun (Biological Diversity Research Institute) ;
  • Kim, Jong-Hwan (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Keun-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 이일로 (순천향대학교 해양생명공학과) ;
  • 양현 ((주)생물다양성연구소) ;
  • 김종환 (순천향대학교 해양생명공학과) ;
  • 김근용 (순천향대학교 해양생명공학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Received : 2009.11.18
  • Accepted : 2009.12.21
  • Published : 2009.12.31
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Abstract

A natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa was genetically identified by sequence analyses of nuclear recombination activating gene 1 (RAG1) and mitochondrial cytochrome c oxidase I (COI) genes. Out of 850 base positions of RAG1, a total of 23 nucleotide substitutions were detected between the two parental species, whereas the electropherogram of the natural hybrid displayed double peaks at all of the 23 positions, which reflects their simple Mendelian inheritance pattern. Meanwhile, comparison of partial sequences of mitochondrial genes (COI in this study), which are well characterized by the maternal inheritance pattern, revealed that the maternal species of the hybrid was C. tetralineata because of their 100% sequence identity.

줄종개(Cobitis tetralineata)와 왕종개(Iksookimia longicorpa)간 자연잡종으로 추정되는 개체를 유전적으로 동정하기 위하여 핵 recombination activating gene 1 (RAG1)과 미토콘드리아 cytochrome c oxidase I (COI) 유전자들의 염기서열을 분석하였다. RAG1 염기서열을 분석한 결과 850 bp 중에서 두 친어종들 간에 총 23개의 치환이 관찰되었고, 자연잡종 개체의 electropherogram에서는 이들 치환이 관찰된 모든 위치들에서 double peak들이 관찰되어, 멘델의 유전법칙을 따랐다. 그리고 모계를 통해 자손에게 유전되는 특징을 가지는 미토콘드리아 유전자들 중에서 COI 염기서열을 비교한 결과 잡종 개체는 줄종개와 염기서열이 100% 일치하여 그 모계는 줄종개임이 명확히 밝혀졌다.

Keywords

References

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