Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Distinct Roles for JNK1 and JNK3 During TNF-α- or Etoposide-Induced Apoptosis in HeLa Cells

  • Ham, Young-Mi (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University) ;
  • Lim, Jin-Hee (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University) ;
  • Lee, Seung-Ki (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University)
  • Received : 2008.12.17
  • Accepted : 2009.10.12
  • Published : 2009.12.31
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Abstract

Here, we show that JNK1 and JNK3 have different roles in ${\alpha}-$ or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited $TNF-{\alpha}-$ or etoposide-induced apoptosis, while dominant negative JNK3 promoted $TNF-{\alpha}-$ or etoposide-induced apoptosis. During $TNF-{\alpha}$-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in $TNF-{\alpha}$-induced apoptosis originates before the apoptotic machinery is triggered.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology, Korea Science and Engineering Foundation

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