Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay

증강 화학발광 기법을 이용한 horseradish peroxidase(HRP)의 검량

  • Received : 2008.01.23
  • Accepted : 2008.11.10
  • Published : 2009.01.30

Abstract

Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

Keywords

Acknowledgement

Supported by : 관동대학교

References

  1. Borgesa, E. P. and Reis, B. F. (2005). An enzymatic flowinjection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process. Journal of Brazilian Chemical Society, 16, pp. 1226-1232 https://doi.org/10.1590/S0103-50532005000700021
  2. Cormier, M. J. and Prichard, P. M. (1968). An investigation of the mechanism of the luminescent peroxidation of luminol by stopped flow techniques. Journal of Biological Chemistry, 243, pp. 4706-4714
  3. Ii, M., Yoshida, H., Aramaki, Y., Masuya, H., Hada, T., Terada, M., Hatanaka, M., and Ichimori, Y. (1993). Improved enzyme immunoassay for human basic fibroblast growth factor using a new enhanced chemiluminescence system. Biochemical and Biophysical Research Communications, 193, pp. 540-545 https://doi.org/10.1006/bbrc.1993.1657
  4. Ilyina, A. D., Martinez Hernández, J. L., Mauricio Benavides, J. E., Lopez Luján, B. H., Bogatecheva, E. S., Romero Garcia, J., and Rodiriguez Martínez, J. (2003). Determination of phenol using an enhanced chemiluminescent assay. Luminescence, 18, pp. 31-36 https://doi.org/10.1002/bio.698
  5. Klibanov, A. M., Tu, T. M., and Scott, K. P. (1983). Peroxidase catalyzed removal of phenols from coal-conversion wastewaters. Science, 221, pp. 259-261
  6. Kricka, L. K. and Thorpe, G. H. G. (1983). Chemiluminescent and bioluminescent methods in analytical chemistry. Analyst, 108, pp. 1274-1293 https://doi.org/10.1039/an9830801274
  7. Li, W., Chen, K., Zhao, Y., Chen, L., Toselli, P., Chou, I. N., and Stone, P. (2004). Transcriptional perturbation of lysyl oxidase by cigarette smoke condensate in cultured lung fibroblasts. Toxicologist, 78, pp. 262 (abstract)
  8. Motsenbocker, M. A. and Kondo, K. (1994). Improvement to enhanced horseradish peroxidase detection sensitivity. Journal of Bioluminescence and Chemiluminescence, 9, pp. 15-20 https://doi.org/10.1002/bio.1170090104
  9. Nicell, J. A., Bewtra, J. K., Biswas, N., St. Pierre, C., and Taylor, K. E. (1993). Enzyme catalyzed polymerization and precipitation of aromatic compounds from aqueous solution. Canadian Journal of Civil Engineering, 20, pp. 725-735 https://doi.org/10.1139/l93-097
  10. Osman, A. M., Zomer, G., Laane, C., and Hilhorst, R. (2000). Comparative studies of the chemiluminescent horseradish peroxidase-catalyzed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems. Luminescence, 15, pp. 189-197 https://doi.org/10.1002/1522-7243(200005/06)15:3<189::AID-BIO585>3.0.CO;2-A
  11. Thorpe, G. H. G., Gillespie, E., Haggart, R., Kricka, L. J., and Whitehead, T. P. (1984). Analytical applications of bioluminescence and chemiluminescence. Academic, London
  12. Thorpe, G. H. G., Kricka, L. J., Moseley, S. B., and Whitehead, T. P. (1985a). Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays. Clinical Chemistry, 31, pp. 1335-1341
  13. Thorpe, G. H. G., Kricka, L. J., Gillespie, E., Moseley, S., Amess, R., Baggett, N., and Whitehead, T. P. (1985b). Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazide by 6-hydroxybenzothiazoles. Analytical Biochemistry, 145, pp. 96-100 https://doi.org/10.1016/0003-2697(85)90332-X
  14. Thorpe, G. H. G. and Kricka, L. J. (1986). Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase. Methods Enzymol., 133, pp. 331-353 https://doi.org/10.1016/0076-6879(86)33078-7
  15. Wu, Y., Taylor, K. E., Biswas, N., and Bewtra, J. K. (1997). Comparison of additives in the removal of phenolic compounds by peroxidase-catalyzed polymerization. Water Research, 31, pp. 2699-2704 https://doi.org/10.1016/S0043-1354(97)00119-X
  16. Yasaei, P. M., Yang, G. C., Warner, C. R., Daniels, D. H., and Kau, Y. (1996). Singlet oxygen oxidation of lipids resulting from photochemical sensitizers in presence of antioxidants. Journal of the American Oil Chemists' Society, 73, pp. 1177-1181 https://doi.org/10.1007/BF02523381
  17. Yeh, H. C. and Lin, W. Y. (2002). Enhanced chemiluminescence for the oxidation of luminol with m-chloroperoxybenzoic acid catalyzed bymicroperoxidase. Analytical and Bioanalytical Chemistry, 372, pp. 525-531 https://doi.org/10.1007/s00216-001-1202-x