Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

A Review of Multivariate Analysis Studies Applied for Plant Morphology in Korea

국내 식물 형태 연구에 사용된 다변량분석 논문에 대한 재고

  • Chang, Kae Sun (Department of Forest Sciences and The Arboretum, College of Agriculture and Life Sciences, Seoul National University) ;
  • Oh, Hana (Department of Forest Sciences and The Arboretum, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Hui (Department of Medicinal Plants Resources, Mokpo National University) ;
  • Lee, Heung Soo (Department of Forest Sciences and The Arboretum, College of Agriculture and Life Sciences, Seoul National University) ;
  • Chang, Chin-Sung (Department of Forest Sciences and The Arboretum, College of Agriculture and Life Sciences, Seoul National University)
  • 장계선 (서울대학교 농업생명과학대학 산림과학부 및 수목원) ;
  • 오하나 (서울대학교 농업생명과학대학 산림과학부 및 수목원) ;
  • 김휘 (목포대학교 응용생명공학부 생약자원전공) ;
  • 이흥수 (서울대학교 농업생명과학대학 산림과학부 및 수목원) ;
  • 장진성 (서울대학교 농업생명과학대학 산림과학부 및 수목원)
  • Received : 2009.04.29
  • Accepted : 2009.06.19
  • Published : 2009.06.30
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Abstract

A review was given of the role of traditional morphometrics in plant morphological studies using 54 published studies in three major journals and others in Korea, such as Journal of Korean Forestry Society, Korean Journal of Plant Taxonomy, Korean Journal of Breeding, Korean Journal of Apiculture, Journal of Life Science, and Korean Journal of Plant Resources from 1997 to 2008. The two most commonly used techniques of data analysis, cluster analysis (CA) and principal components analysis (PCA) with other statistical tests were discussed. The common problem of PCA is the underlying assumptions of methods, like random sampling and multivariate normal distribution of data. The procedure was intended mainly for continuous data and was not efficient for data which were not well summarized by variances or covariances. Likewise CA was most appropriate for categorical rather than continuous data. Also, the CA produced clusters whether or not natural groupings existed, and the results depended on both the similarity measure chosen and the algorithm used for clustering. An additional problems of the PCA and the CA arised with both qualitative and quantitative data with a limited number of variables and/or too few numbers of samples. Some of these problems may be avoided if a certain number of variables (more than 20 at least) and sufficient samples (40-50 at least) are considered for morphometric analyses, but we do not think that the methods are all mighty tools for data analysts. Instead, we do believe that reasonable applications combined with focus on objectives and limitations of each procedure would be a step forward.

최근 10여년간(1997-2008년) 국내 식물 형태관련 연구논문중 주요 3개 학회지(한국임학회지, 한국식물분류학회지, 한국육종학회지)와 기타학회지(한국자원식물학회지, 생명과학학회지, 약용작물학회지, 한국양봉학회지, 원예과학기술지, 원예학회지) 등에 발표된 다변량 분석 54개 논문을 재검토하였다. 이들 논문에서 주성분 분석(PCA), 유집분석(CA)이 가장 많이 사용되었는데 본 연구는 이와 관련된 분석방법과 기초 통계 분석의 문제점을 검토하였다. 주성분 분석의 문제점은 기존 표본에 추출과 자료의 정규분포성에 대한 가설을 무시하는 것으로서 분산과 공분산을 구할 수 없는 정성형질을 섞어 사용하여 주성분 분석의 결과를 왜곡하여 분석하는 것이었다. 또한, 유집분석은 분석방법론에 따라 상당한 차이를 보여 정량적 형질 보다는 정성적 형질로 계수화하여 분석함을 권장하지만 대부분 정량적 형질로 분석하여 실제 상호 분석 대상군 간의 관계가 형성되지 않는 계단상 모양을 보였다. 또한, 일부 연구논문에서는 통계적 가설을 무시하고 임의로 자료를 해석하는 것도 자주 발견되는 문제점 중 하나였다. 주성분분석에서 주의해야 할 점은 주로 정량적 형질 사용이 권장되며, 이외 일정 수 이상의 변량(20여개 이상)과 적절한 개체수(40-50개 이상)가 필요하다는 것이다. 유집분석(혹은 군집분석)은 분석 대상을 강제로 유집시키는 성향이 있어 통계적 검증 방법을 사용하지 않고 단순히 거리 계산법 혹은 알고리즘 분석 방법에 의해 결과에 상당한 차이를 보이기 때문에 정량적 형질만을 근간으로 한 유집분석은 자제할 필요가 있다. 모든 자료를 쉽게 분석하기 위해서 단순히 통계적 방법론에 의존하기 보다는 연구목적과 일치한 분석 방법론을 적용하고자 하는 노력이 필수적이다.

Keywords

Acknowledgement

Supported by : 산림청

References

  1. Ahn, Y.S., Kim, S.H., Jung, H.K., Jang, Y.S., Choi, Y.C., and Oh, K.I. 2002. The variation of leaf characters among natural populations of Kalopanax septemlobus Koidz. Journal of Korean Forestry Society 91(6): 755-764 (in Korean)
  2. Cho, S.K., Choi, B.H., and Kim, J.H. 1997. Morphological variations and taxonomy of Indigofera kirilowii and I. koreana (Leguminosae). Korean Journal of Plant Taxonomy 27(1): 73-87 (in Korean)
  3. Chung, K.H. 1999. Morphological characteristics and principal component analysis of plums. Korean Journal of Horticultural Science & Technology 17(1): 23-28 (in Korean)
  4. Henderson, A. 2006. Traditional morphometrics in plant systematics and its role in palm systematics. Botanical Journal of the Linnean Society 151(1): 103-111 https://doi.org/10.1111/j.1095-8339.2006.00526.x
  5. Hwang, S.H., Park, S.J., and Kim, J.H. 2007. A numerical taxomony of Korean Ilex (Aquifoliaceae). Korean Journal of Plant Taxonomy 37(4): 401-418 (in Korean
  6. Jang, D.H., Suh, S.J., Baek, S.B., Kim, J.G., and Nam, J.H. 2001. Efficiency of morphological traits and DNA markers in classification of Korean barley cultivars. Korean Journal of Breeding 33(4): 300-305(in Korean)
  7. Jang, Y.S. Lee, M.H. and Lee, B.S. 2006. Flower morphological characteristics and classification of genus Crataegus. Korean Journal of Apiculture 21(1): 11-18 (in Korea)
  8. James, F.C., and McCulloch, C.E. 1990. Multivariate analysis in ecology and systematics: Panacea or pandora's box? Annual Review of Ecological System 21: 129-166
  9. Kim, C.S., Moon, M.O., Cheong, E.J., and Byun, G.O. 2005. Evaluation of leaf morphology for distinguishing Prunus (Rosaceae) from Jeju, Korea. Korean Journal of Plant Taxonomy 35(2): 81-98 (in Korean)
  10. Kim, C.Y., Pyon, J.Y. and Lee J.H. 1999. Variation of characteristics and principal component analysis of collected colored rice cultivars. Korean Journal of Plant Resources 12(3): 186-191 (in Korean)
  11. Kim, J.H., Jang, W.S., Kyung, H.Y., Xuan, Y., Davaasuren, Y.E., Sim, E.J., Lee, J.K.,Choi, Y.S., Hiramatsu, M., Kim, K.W and Yoo, K.O. 2006. A Principal Component Analysis for the Morphological Characters of Diploid and Triploid Populations of Lilium lancifolium in Korea. Korean Journal of Plant Resources 19(2): 300-307 (in Korean)
  12. Kim, N.Y., Kim, H.S., Kim, S.Y., and Park, W.G. 2006. Studies on morphological variation among provenances of a rare Rhododendron micranthum in Korea. Journal of Korean Forestry Society 95(1): 55-59 (in Korean)
  13. Kim, S.H., Jang, Y.S., Chung, H.G., Park, H.S., and Cho, K.J. 2003. Leaf morphological characteristics of Sorbus commixta Hedl. Selected populations. Journal of Korean Forestry Society 92(5): 488-496 (in Korean)
  14. Kim, S.H., Jang, Y.S., Han, J.G., Seo, H.W., Park, I.H., and Cho K.J. 2007. Leaf characteristics and genetic relationships analysis among four species in the genus Sorbus. Korean Journal of Breeding 39(4): 427-434 (in Korean)
  15. Kim, S.T., Kim, M.H., and Park, C.W. 2000. A systematic study on Fallopia section Fallopia (Polygonaceae). Korean Journal of Plant Taxonomy 30(1): 35-54 (in Korean)
  16. Koo, Y.B., Kim, I.S., Y.K., Yeo J.K., Lee, J.C., and Tak, W.S. 2001. Characteristics of Korean aspen (Populus davidiana Dode) progenies from artificial mating. Korean Journal of Breeding 33(4): 311-317 (in Korean)
  17. Lee, I.S., and Park, J.O. 2003. Assessment and classification of Korean indigenous corn lines by application of principal component analysis. Journal of Life Science 13(3): 343-348 (in Korean)
  18. Lee, J.K. 2002. Morphological variation of Perilla crop and weedy collections in Korea. Korean Journal of Breeding 34(1): 27-36 (in Korean)
  19. Lee, S.R. and Park, J.O. 2007. Systematie study on the Aconitum alboviolaccum complex (Ranunculaceae) in Korea Journal of Plant Taxonomy 37(1): 477-502 (in Korea)
  20. Park, H.S., Lee, U., and Jung, M.S. 2008. Classification of Chionanthus retusa for flower morphological characteristics. Korean Journal of Apiculture 23(2): 133-137 (in Korean)
  21. Park, S.H., Lee, J.K., and Kim, J.H. 2007. A morphological study of Symplocaceae in Korea. Korean Journal of Plant Taxonomy 37(3): 255-273 (in Korean)
  22. Song, J.H., Park, M.H., Moon, H.K., Han, S.U., and Yi, J.S. 2000. The variation of leaf form of natural populations of Quercus variabilis in Korea. Journal of Korean Forestry Society 89(5): 666-676 (in Korean)
  23. Song, M.J., and Kim, H. 2005. Taxonomic identities of Cirsium setidens, C. chanroenicum and C. toraiense. Korean Journal of Plant Taxonomy 35(4): 227-245 (in Korean)
  24. Song, M.J., and Kim, H. 2006. Numerical taxonomy of Cirsium japonicum complex (Asteraceae) in Korea. Korean Journal of Plant Taxonomy 36(4): 279-292 (in Korean)
  25. Song, M.J., and Kim, H. 2007. Taxonomic study on Cirsium Miller (Asteraceae) in Korea based on external morpholgy. Korean Journal of Plant Taxonomy 37(1): 17-40 (in Korean)
  26. Woo, K.S. 2003. Studies on the variation of needle, cone, and seed characteristics related to the conservation of genetic resources of Pinus densiflora for. multicaulis. Journal of Korean Forestry Society 92(1): 8-18 (in Korean)
  27. Yoo, K.O., Kim, W.B., Park, H.J., Lim, S.C., Lee, M.C., and Jang, H.T. 1998. External morphology and numerical taxonomy among habitat of Allium victorialis var. platyphyllum. Korean Journal of Plant Resources 11(2): 210-216 (in Korean)
  28. Yoo, K.O., Lee, W.T., and Oh, Y.J. 1999. External Morphology and vegetation of Megaleranthis saniculifolia populations in four different habitats. Korean Journal of Plant Resources 12(4): 312-323 (in Korean)
  29. Yoo, K.O., Lee, W.T., and Ryu, S.Y. 2000. External morphology and numerical taxonomy of Hanabusaya asiatica populations in different habitats. Korean Journal of Plant Resources 13(1): 80-88 (in Korean)