대한수의학회지 (Korean Journal of Veterinary Research)

  • 제49권4호
  • /
  • Pages.285-290
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  • 2009
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis

  • Lee, Ji Hye (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Keun Jung (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Seon A (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Li, Xiaoxia (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Eun Young (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, Hyun Ju (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong Chun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Hye Jin (Haemaru Small Animal Referral Hospital) ;
  • Park, Byung Kwon (College of Industrial Science, Kongju National University) ;
  • Kim, Min Kyu (College of Agriculture and Life Sciences, Chungnam National University)
  • 심사 : 2009.12.02
  • 발행 : 2009.12.31
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초록

This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{\times}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.

키워드

참고문헌

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