Anti-mycoplasmal and anti-inflammatory effect of Origanum vulgare extract against Mycoplasma hyopneumoniae

Mycoplasma hyopneumoniae에 대한 Origanum vulgare 추출물의 항마이코플라즈마 및 항염증 효과

  • Choi, Myung-Jin (College of Veterinary Medicine, Kyungpook National University) ;
  • Hwang, Mi-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Jae-Young (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Joong-Su (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2009.09.02
  • Published : 2009.09.30

Abstract

In the present study, ten herbal extracts, Citrus unshiu Markovich, root and stem of Berberis koreana, Morus alba, Dendrobium moniliforme, Aster gramineus, A. scabar, Alisma canaliculatum, Fallopia japonica and Origanum (O.) vulgare were determined to examine anti-mycoplasmal activity. Among them, O. vulgare extract (OVE) showed strong anti-mycoplasmal activity and was analyzed by gaschromatography/ mass spectrometry (GC/MS). As the results, OVE was consisted of carvacrol (68.78%), o-cymene (9.80%), terpinene (7.61%) and thymol (4.03%) as main ingredients. To investigate inflammatory activity by intact pathogenic Mycoplasma hyopneumoniae (M. hyo) at 30 $\mu$g/ml, we examined induced transcription of proinflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-a, interleukin (IL)-1, IL-6 and inducible nitric oxide synthase in RAW 264.7 cells. With the above results, we further investigated whether OVE could reduce inflammation induced by M. hyo at minimal inhibitory concentration. The result showed that 32 $\mu$g/ml of OVE inhibited nitric oxide production by 60%. This study also evaluated the combination of OVE with antibacterials against M. hyo for application. Based on these results, it could be concluded that M. hyo induces inflammation in RAW 264.7 cells and OVE protects this inflammation, indicating that OVE may be useful for industrial animals.

Acknowledgement

Supported by : 한국학술진흥재단

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