Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Gamma Irradiation on Chemical Composition, Antinutritional Factors, Ruminal Degradation and In vitro Protein Digestibility of Full-fat Soybean

  • Taghinejad, M. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch Islamic Azad University) ;
  • Nikkhah, A. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch Islamic Azad University) ;
  • Sadeghi, A.A. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch Islamic Azad University) ;
  • Raisali, G. (Radiation Applications Research School, Nuclear Science and Technology Research Institute) ;
  • Chamani, M. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch Islamic Azad University)
  • Received : 2008.10.03
  • Accepted : 2009.01.07
  • Published : 2009.04.01
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Abstract

The aim of this study was to evaluate the effects of gamma irradiation (${\gamma}$-irradiation) at doses of 15, 30 and 45 kGy on chemical composition, anti-nutritional factors, ruminal dry matter (DM) and crude protein (CP) degradibility, in vitro CP digestibility and to monitor the fate of true proteins of full-fat soybean (SB) in the rumen. Nylon bags of untreated or ${\gamma}$-irradiated SB were suspended in the rumens of three ruminally-fistulated bulls for up to 48 h and resulting data were fitted to a nonlinear degradation model to calculate degradation parameters of DM and CP. Proteins of untreated and treated SB bag residues were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Digestibility of rumen undegraded CP was estimated using the three-step in vitro procedure. The chemical composition of raw and irradiated soybeans was similar. Results showed that phytic acid in ${\gamma}$-irradiated SB at dose of 30 kGy was eliminated completely. The trypsin inhibitor activity of 15, 30 and 45 kGy ${\gamma}$-irradiated SB was decreased (p<0.01) by 18.4, 55.5 and 63.5%, respectively. From in sacco results, ${\gamma}$-irradiation decreased (p<0.05) the washout fractions of DM and CP at doses of 30 and 45 kGy, but increased (p<0.05) the potentially degradable fractions. Gamma irradiation at doses of 15, 30 and 45 kGy decreased (p<0.05) effective degradability of CP at a rumen outflow rate of 0.05 $h^{-1}$ by 4.4, 14.4 and 26.5%, respectively. On the contrary, digestibility of ruminally undegraded CP of irradiated SB at doses of 30 and 45 kGy was improved (p<0.05) by 12 and 28%, respectively. Electrophoretic analysis of untreated soybean proteins incubated in the rumen revealed that ${\beta}$-conglycinin subunits had disappeared at 2 h of incubation time, whereas the subunits of glycinin were more resistant to degradation until 16 h of incubation. From the SDS-PAGE patterns, acidic subunits of 15, 30 and 45 kGy ${\gamma}$-irradiated SB disappeared after 8, 8 and 16 h of incubation, respectively, while the basic subunits of glycinin were not degraded completely until 24, 48 and 48 h of incubation, respectively. It was concluded that ${\gamma}$-irradiated soybean proteins at doses higher than 15 kGy could be effectively protected from ruminal degradation.

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References

  1. Abu, J. O., K. Muller, K. G. Duodu and A. Minnaar. 2006. Gamma irradiation of cowpea (Vigna unguiculata K. Walp) flours and pastes: Effects on functional, thermal and molecular properties of isolated protein. Food Chem. 95:138-147 https://doi.org/10.1016/j.foodchem.2004.12.040
  2. AECL Technical Publication 1984. Operators and Accessories Manual of Gammacell 220
  3. AOAC. 1995. Official methods of analysis, 16th ed. Association of Official Analytical Chemists, Arlington, Virginia
  4. Aldrich, C. G., S. Ingram and J. R. Coldfelter. 1997. Assessment of post-uminal amino acid digestibility of roasted and extruded whole soybeans with the precision-fed rooster assay. J. Anim. Sci. 75:3046-3051
  5. Al-Kaiesy, M. T., H. A. Abdul-Kader, M. H. Mohammad and A. H. Saeed. 2003. Effect of $\gamma$-irradiation on antinutritional factors in broad bean. Radiat Phys. Chem. 67:493-496 https://doi.org/10.1016/S0969-806X(03)00091-4
  6. Arieli, A., 1998. Whole cottonseed in dairy cattle feeding: a review. J. Dairy Sci. 72:97-110
  7. Bhat, R., K. R. Sridhar and K. T. Yokotani. 2007. Effect of ionising radiation on antinutritional features of velvet seed bean (Mucuna pruriens). Food Chem. 103:860-866 https://doi.org/10.1016/j.foodchem.2006.09.037
  8. Calsamiglia, S. and M. D. Stern. 1995. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. J. Anim. Sci. 73:1459-1465
  9. Chen, P., P. Ji and S. L. Li. 2008. Effects of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation, performance and milk composition and fatty acid profile in early lactation dairy cow. Asian-Aust. J. Anim. Sci. 21:204-213
  10. Cho, Y. and K. B. Song. 2000. Effect of $\gamma$-irradiation on molecular properties of BSA and $\beta$-lactoglobulin. J. Biochem. Mol. Biol. 33:133-137
  11. De Boland, A. R., G. B. Garner and B. L. O'Dell. 1975. Identification and properties of phytate in cereal grains and oil seed products. J. Agric. Food Chem. 23:1186-1189 https://doi.org/10.1021/jf60202a038
  12. De Toledo, T. C. F., S. G. Canniatti-Brazaca, V. Artur and S. M. S. Piedade. 2007. Effects of gamma radiation on total phenolics, trypsin and tanin inhibitors in soybean grains. Radiat. Phys. Chem. 76:1653-1656 https://doi.org/10.1016/j.radphyschem.2007.02.001
  13. Diaa El-Din, M. and H. Farag. 1998. The nutrietive value for chicks of full-fat soybeans irradiated at up to 60 kGy. J. Anim. Feed Sci. Technol. 73:319-328 https://doi.org/10.1016/S0377-8401(98)00148-5
  14. Diehl, J. F. 2002. Food irradiation-past, present and future. Radiat. Phys. Chem. 63:211-215 https://doi.org/10.1016/S0969-806X(01)00622-3
  15. Farkas, J. 2006. Irradiation for better foods. Trends in Food Science and Technology 17:148-152 https://doi.org/10.1016/j.tifs.2005.12.003
  16. Food and Drug Administration 1981. Irradiation in the production, processing, and handling of food; final rule. 21CFR Part 179. Federal Register 51:13376-13399
  17. Gaber, M. H. 2005. Effect of γ-irradiation on molecular properties of bovine serum albumin. J. Biosci. Bioengin. 100:203-206 https://doi.org/10.1263/jbb.100.203
  18. Gharaghani, H., M. Zaghari, G. shahhoseini and H. Moravej. 2008. Effect of gamma irradiation on antinutritional factors and nutritional value of canola meal for broiler chikens. Asian-Aust. J. Anim. Sci. 21:1479-1485
  19. Ham, J. S., S. G. Jeog, S. G. Lee, G. S. Han, A. Jang, Y. M. Yoo, H. S. Chase, D. H. Kim, H. J. Kim, W. K. Lee and C. Jo. 2009. Quality of irradiated plain yogurt during storage at different temperatures. Asian-Aust. J. Anim. Sci. 22:289-295
  20. Kopecny, J. and R. J. Wallace. 1982. Cellular location and some properties of proteolytic enzymes of rumen bacteria. Appl. Environ. Microbiol. 43:1026-1033
  21. Krishnamoorthy, U., T. V. Muscato, C. J. Sniffen and P. J. Van Soest. 1982. Nitrogen fractions in selected feedstuffs. J. Dairy Sci. 65:217-229 https://doi.org/10.3168/jds.S0022-0302(82)82180-2
  22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680 https://doi.org/10.1038/227680a0
  23. Lacroixa, M., T. C. Lea, B. Ouattaraa, H. Yua, M. Letendrea, S. F. Sabatoc, M. A. Mateescub and G. Patterson. 2002. Use of $\gamma$-irradiation to produce films from whey, casein and soya proteins: structure and functionals characteristics. Radiat. Phys. Chem. 63:827-832 https://doi.org/10.1016/S0969-806X(01)00574-6
  24. Lee, S. L., M. S. Lee and K. B. Song. 2005. Effect of $\gamma$-irradiation on the physicochemical properties of gluten films. Food Chem. 92:621-625 https://doi.org/10.1016/j.foodchem.2004.08.023
  25. Liener, I. E. 1994. Implications of antinutritional components in soybean foods: Critical Reviews. Food Sci. Nut. 34:31-67 https://doi.org/10.1080/10408399409527649
  26. Murray, R. K., D. K. Granner, P. A. Mayes and V. W. Rodwell. 2003. Harper's Biochemistry, 26th ed. McGraw-Hill, New York, New York
  27. National Research Council 1996. Nutrient requirements of beef cattle. 7th Ed. National Academy Press, Washington, DC
  28. Nocek, J. E. 1988. In situ and other methods to estimate ruminal protein and energy digestibility: a review. J. Dairy Sci. 71:2051-2069 https://doi.org/10.3168/jds.S0022-0302(88)79781-7
  29. $\O$rskov, E. R. and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. (Camb.) 92:499-503 https://doi.org/10.1017/S0021859600063048
  30. Ressouany, M., C. Vachon and M. Lacroix. 1998. Irradiation dose and calcium effect of the mechanical properties of cross-linked caseinate films. J. Agric. Food Chem. 46:1618-1623 https://doi.org/10.1021/jf970805z
  31. Romagnolo, E., C. E. Polan and W. E. Barbeau. 1990. Degradability of soybean meal protein fractions as determined by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Dairy Sci. 73:2379-2385 https://doi.org/10.3168/jds.S0022-0302(90)78921-7
  32. Roy, D. N. and R. V. Bhat. 1974. Trypsin inhibitor content in some varieties of soybean (Glycine max L.) and sunflower seeds (Hellcanthus annuus L.). J. Sci. Food Agric. 25:765 https://doi.org/10.1002/jsfa.2740250704
  33. Sadeghi, A. A., A. Nikkhah, P. Shawrang and M. M. Shahrebabak. 2006. Protein degradation kinetics of untreated and treated soybean meal using SDS-PAGE. Anim. Feed Sci. Technol. 126:121-133 https://doi.org/10.1016/j.anifeedsci.2005.05.026
  34. SAS Institute Inc. 1996. Statistical Analysis System (SAS) User's Guide. SAS Institute, Cary, NC, USA
  35. Scott, T. A., D. K. Combs and R. R. Grummer. 1991. Effects of roasting, extrusion, and particle size on the feeding value of soybeans for dairy cattle. J. Dairy Sci. 74:2555-2562 https://doi.org/10.3168/jds.S0022-0302(91)78433-6
  36. Shawrang, P., A. Nikkhah, A. Zare-Shahneh, A. A. Sadeghi, G. Raisali and M. Moradi-Shahrebabak. 2007. Effects of gamma irradiation on protein degradation of soybean meal. Anim. Feed Sci. Technol. 134:140-151 https://doi.org/10.1016/j.anifeedsci.2006.05.019
  37. Shawrang, P., A. Nikkhah, A. Zare-Shahneh, A. A. Sadeghi, G. Raisali and M. Moradi-Shahrebabak. 2008. Effects of gamma irradiation on chemical composition and ruminal protein degradation of canola meal. Radiat. Phys. Chem. 77:918-922 https://doi.org/10.1016/j.radphyschem.2008.03.006
  38. Siddhuraju, P., H. P. S. Makkar and K. Becker. 2002. The effect of ionising radiation on antinutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chem. 78:187-205 https://doi.org/10.1016/S0308-8146(01)00398-3
  39. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597 https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  40. Van Soest, P. J. 1994. Nutritional ecology of the ruminants. 2nd Edition. Cornell University Press. NY. USA. p. 293
  41. Wallace, R. J. 1985. Adsorption of soluble proteins to rumen bacteria and the role of adsorption in proteolysis. Br. J. Nutr. 53:399-408 https://doi.org/10.1079/BJN19850047
  42. Wang, Y., T. A. McAllister, M. D. Pickard, Z. Xu, L. M. Rode and K. J. Cheng. 1999. Effect of micronizing full fat canola seed on amino acid disappearance in the gastrointestinal tract of dairy cows. J. Dairy Sci. 82:537-544 https://doi.org/10.3168/jds.S0022-0302(99)75265-3
  43. Yamamoto, O. 1992. Effect of radiation on protein stability. In: Stability of protein pharmaceuticals (Ed. T. J. Ahern and M. C. Manning), New York. NY, USA: Plenum Press. pp. 361-419

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