Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of IGF-I and EGF Supplemented to PZM3 Culture Medium on the Development of Porcine Embryos In vitro

  • Kim, J.Y. (Infertility Medical Center of CHA General Hospital) ;
  • Park, M.C. (Infertility Medical Center of CHA General Hospital) ;
  • Kim, S.B. (Infertility Medical Center of CHA General Hospital) ;
  • Park, H.D. (Department of Biotechnology, Daegu University) ;
  • Lee, J.H. (College of Medicine, CHA University) ;
  • Kim, Jae-Myeoung (College of Medicine, CHA University)
  • Received : 2009.01.19
  • Accepted : 2009.04.22
  • Published : 2009.08.01
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Abstract

This study investigated the effects of IGF-I and EGF on the development of blastocysts or hatched blastocysts during the in vitro culture of embryos from immature porcine oocytes. After the in vitro maturation and fertilization of cumulus-oocyte complexes (COCs) and their culture in vitro in PZM3 medium, we examined the embryo development rate for 168 h. When different concentrations of IGF-I (0, 1, 10, 20 ng/ml) were supplemented to fertilized porcine embryos in vitro, there were no significant differences in cleavage rate, blastocyst development rate or blastocyst hatching rate among the treated groups. On the other hand, when different concentrations of EGF (0, 1, 10, 20 ng/ml) were supplemented to the in vitro culture medium, blastocyst development rate was highest in the group in which EGF was not supplemented and, specifically, it was higher than in the 20 ng/ml treatment group (p<0.05). When 10 ng/ml IGF-I and 1 ng/ml EGF were supplemented separately or simultaneously, there were no significant differences among the treated groups in blastocyst hatching rate and the number of cells in each condition. This study demonstrated that the addition of IGF-I and EGF into PZM3 medium did not enhance development of the blastocyst stage and total cell number in blastocysts.

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References

  1. Abeydeera, L. R. and B. N. Day. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734 https://doi.org/10.1095/biolreprod57.4.729
  2. Abeydeera, L. R., W. H. Wang, T. C. Cantley, A. Rieke, R. S. Prather and B. N. Day. 1998. Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. Dev. 51:395-401 https://doi.org/10.1002/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y
  3. Adashi, E. Y., C. E. Resnick, A. J. D' Ercole, M. E. Svoboda and J. J. Van Wyk. 1985. Insulin-like growth factors as intraovarian regulators of granulose cell growth and function. Endocr. Rev. 3:400-420
  4. Ali, A. A., J. F. Bilodeau and M. A. Sirard. 2003. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology 59:939-949 https://doi.org/10.1016/S0093-691X(02)01125-1
  5. Byun, T. H. and S. H. Lee. 1992. Morphological and cellular criteria ovaries, follicles and oocytes for in vitro maturation in the pig. Korean J. Emb. Tran. 7:97-110
  6. Camous, S., Y. Heyman, W. Menezo and Y. Menezo. 1984. Cleavage beyond the block stage and survival after transfer of early bovine embyos cultured with trophoblastic vesicles. J. Reprod. Fertil. 72:479-485 https://doi.org/10.1016/0093-691X(94)90278-X
  7. Chang, H. S., W. T. Cheng, H. K. Wu and K. B. Choo. 2000. Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development. Mol. Reprod. Dev. 56:331-335 https://doi.org/10.1002/1098-2795(200007)56:3<331::AID-MRD1>3.0.CO;2-K
  8. Chollier, M., C. O'Neill, A. J. Ammit and D. M. Saunders. 1990. Measurement of human embryo-derived platelet-activating factor (PAF) using a quantitative bioassay of platelet aggregation. Hum. Reprod. 5:323-328 https://doi.org/10.1093/oxfordjournals.humrep.a137098
  9. Coskun, S., A. Sanbuissho, Y. C. Lin and Y. Rikihosa. 1991. Fertilizability and subsequent developmental ability of bovine oocytes matured in medium containing epidermal growth factor (EGF). Theriogenology 36:485-494 https://doi.org/10.1016/0093-691X(91)90477-U
  10. Das, K., L. E. Stout, H. C. Hensleigh, G. E. Tagatz, W. R. Phipps and B. S. Leung. 1991. Direct positive effect of epidermal growth factor on the cytoplasmic maturation of mouse and human oocytes. Fertil. Steril. 55:1000-1004 https://doi.org/10.1016/0093-691X(95)00170-D
  11. Davies, S., M. C. Richardson, F. W. Anthony, D. Mukhtar and I. T. Cameron. 2004. Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube. Mol. Hum. Reprod. 10:935-939 https://doi.org/10.1093/molehr/gah124
  12. Demeestere, I., C. Gervy, J. Centner, F. Devreker, Y. Englert and A. Delbaere. 2004. Effect of insulin-like growth factor-I during preantral follicular culture on steroidogenesis, in vitro oocyte maturation and embryo development in mice. Biol. Reprod. 70:1664-1669 https://doi.org/10.1095/biolreprod.103.023317
  13. Desai, N., J. Lawson and J. Goldfarb. 2000. Assessment of growth factor effects on post-thaw development of cryopreserved mouse morulae to the blastocyst stage. Hum. Reprod. 15:410-418 https://doi.org/10.1093/humrep/15.2.410
  14. Devreker, F. and K. Hardy. 1997. Effects of glutamine and taurine on preimplantation development and cleavage of mouse embryos in vitro. Biol. Reprod. 57:921-928 https://doi.org/10.1095/biolreprod57.4.921
  15. Doherty, A. S., G. L. Temeles and R. M. Schultz. 1994. Temporal pattern of IGF-I expression during mouse preimplantation embryo genesis. Mol. Reprod. Dev. 37:21-26 https://doi.org/10.1002/mrd.1080370104
  16. Down, S. M. 1989. Specificity of epidermal growth factor action on maturation of the murine oocytes and cumulus oophorus in vitro. Biol. Reprod. 41:371-379 https://doi.org/10.1095/biolreprod41.2.371
  17. Echternkamp, S. E., L. J. Spicer, J. Klindt and R. K. Vernon. 1994. Administration of porcine somatotropin by a sustained-release implant: Effects on follicular growth, concentrations of steroids and insulin-like growth factor I, and insulin-like growth factor binding. J. Anim. Sci. 72:2431 (Abstr.) https://doi.org/10.2527/1994.7292431x
  18. Fabian, D., G. Il'kova, P. Rehak, S. Czikkova, V. Baran and J. Koppel. 2004. Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro. Theriogenology 61:745-755 https://doi.org/10.1016/S0093-691X(03)00254-1
  19. Geisert, R. D., C. Y. Lee, F. A. Simmen, M. T. Zary, A. E. Fliss and F. W.Bazer. 1991. Expression of messenger RNAs encoding insulin-like growth factor-I, II, and insulin-like growth factor binding protein-2 in bovine endometrium during the estrous cycle and early pregnancy. Biol. Reprod. 45:975-983 https://doi.org/10.1095/biolreprod45.6.975
  20. Harper, K. M. and B. G. Brackett. 1993. Bovine blastocysts development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins. Biol. Reprod. 48:409-416 https://doi.org/10.1095/biolreprod48.2.409
  21. Herrler, A., C. A. Krusche and H. M. Beier. 1998. Insulin and Insulin-like growth factor-Ⅰpromote rabbit blastocyst development and prevent apoptosis. Biol. Reprod. 59:1302-1310
  22. Heyman, Y., Y. Menezo, P. Chesne, S.Camous and V. Garnier. 1987. In vitro cleavage of bovine and ovine early embryos: Improved development using co-culture with trophoblastic vesicles. Theriogenology 27:59-68 https://doi.org/10.1016/0093-691X(87)90070-7
  23. Hill, D. J. and J. Hogg. 1989. Growth factors and the regulation of pre-and postnatal growth. Baillieres Clin. Endocrinol. Metab. 3:579-625 https://doi.org/10.1016/S0950-351X(89)80059-X
  24. Hill, D. J. 1992. Peptide growth factor interaction in embryonic and fetal growth. Horm. Res. 38:199-202
  25. Kane, M. T., E. W. Carney and J. E. Ellington. 1992. The role of nutrients, peptide growth factors and co-culture cells in development of preimplantion embryos in vitro. Theriogenology 38:297-313 https://doi.org/10.1016/0093-691X(92)90237-L
  26. KiKuchi, K., A. Onishi, N. Kashiwazaki, M. Iwamoto, J. Noguchi, H. Kaneko, T. Akita and T. Nagai. 2002. Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biol. Reprod. 66:1033-1041 https://doi.org/10.1095/biolreprod66.4.1033
  27. Kim, J. H., K. Niwa, J. M. Lim and K. Okuda. 1993. Effects of phosphate, energy substrates, and amino acids on development of in vitro-matured, in vitro-fertilized bovine oocytes in a chemically defined, protein-free culture medium. Biol. Reprod. 48:1320-1325 https://doi.org/10.1095/biolreprod48.6.1320
  28. Kim, J. Y., S. B. Kim, M. C. Park, H. Park, Y. S. Park, H. D. Park, J. H. Lee and J. M. Kim. 2007. Addition of macromolecules to PZM-3 culture medium on the development and hatching of in vitro porcine embryos. Asian-Aust. J. Anim. Sci. 20:1820-1826 https://doi.org/10.5713/ajas.2007.1820
  29. Kulik, G., A. Klippe and M. J. Weber. 1997. Antiapoptotic signaling by the insulin-like growth factor I receptor, phosphatidylinositol 3-kinase and Akt. Mol. Cell. Biol. 17:1595-1606 https://doi.org/10.1128/MCB.17.3.1595
  30. Kurzawa, R., W. Glabowski, T. Baczkowski and P. Brelik. 2002. Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor-I and II epidermal growth factor, insulin, transferring and selenium. Reprod. Biol. 2:143-162
  31. Larson, S. F. and J. E. Parks. 1990. In vitro maturation and fertilization of bovine oocytes in defined medium. Biol. Reprod. 92:156 (Abstr.)
  32. Lee, E. S. and Y. Fukui. 1995. Effects of various growth factors in a defined culture medium on in vitro development of bovine embryos mature and fertilized in vitro. Theriogenology 44:71-83 https://doi.org/10.1016/0093-691X(95)00149-3
  33. Lee, G. S., H. S. Kim, S. H. Hyun, H. Y. Jeon, D. H. Nam, Y. W. Jeong, S. Kim, J. H. Kim, S. K. Kang, B. C. Lee and W. S. Hwang. 2005. Effect of epidermal growth factor in preimplantation development of porcine cloned embryos. Mol. Reprod. Dev. 71:45-51 https://doi.org/10.1002/mrd.20098
  34. Lee, H. K., C. K. Lee, B. K. Yang, G. J. Jeon, K. D. Choi, S. S. Lee, H. S. Kong and H. Y. Jang. 2004. Expression of the antioxidant enzyme and apoptosis genes in in vitro maturation / in vitro fertilization porcine embryos. Asian-Aust. J. Anim. Sci. 17:33-38 https://doi.org/10.5713/ajas.2004.33
  35. Lee, Y. S., S. A. Ock, S. K. Cho, B. G. Jeon, T. Y. Kang, S. Balasubramanian, S. Y. Choe and G. J. Rho. 2007. Effect of donor cell type and passage on preimplantatin development and apoptosis in porcine cloned embryos. Asian-Aust. J. Anim. Sci. 20:711-717 https://doi.org/10.5713/ajas.2007.711
  36. Lighten, A. D., K. Hardy, R. M. Winston and G. E. Moore. 1997. Expression of mRNA for the insulin-like growth factors and their receptors in human preimplantation embryos. Mol. Reprod. Dev. 47:134-139 https://doi.org/10.1002/(SICI)1098-2795(199706)47:2<134::AID-MRD2>3.0.CO;2-N
  37. Lim, J. M., B. C. Reggio, R. A. Godke and W. Hansel. 1996. Culture of bovine embryos in a dynamic culture system: Effect of bovine oviduct epithelial cells on the development of 8-cell embryos to the blastocyst stage. Annual conf. 199
  38. Lonergan, P., C. Carolan, A. Van Langendonckt, I. Donnay, H. Khati and P. Mermillod. 1996. Role of epidermal growth factor in bovine oocyte maturation and preimplantation embryo development in vitro. Biol. Reprod. 54:1420-1429 https://doi.org/10.1095/biolreprod54.6.1420
  39. Makarevich, A. V., P. Chrenek and P. Flak. 2006. The influence of microinjection of foreign gene into the pronucleus of fertilized egg on the preimplantation development, cell number and diameter of rabbit embryos. Asian-Aust. J. Anim. Sci. 19:171-175
  40. May, J. V., J. P. Frost and D. W. Schomberg. 1988. Differential effects of epidermal growth factor, somatomedian-C/insulinlike growth factor I and transforming growth factor-beta on porcine granulose cell DNA synthesis and cell proliferation. Endocrinology 123:168-179 https://doi.org/10.1210/endo-123-1-168
  41. Minshall, C., S. Arkins, G. G. Freund and K. W. Kelly. 1996. Requirement for phosphatidylinostol 3'-kinase to protect hemopoietic progenitors against apoptosis depends upon the extracellular survival factor. J. Immunol. 156:939-947
  42. Moreira, F., F. F. Paula-Lopes, P. J. Hansen, L. Badinga and W. W. Thatcher. 2002. Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos. Theriogenology 57:895-907 https://doi.org/10.1016/S0093-691X(01)00694-X
  43. Morita, Y., T. F. Manganaro, X. J. Tao, S. Martimbeau, P. K. Donaho and J. L.Tilly. 1999. Requirement for phosphatidylinositol 3-kinase in cytokinemediated germ cell survival during fetal oogenesis in the mouse. Endocrinology 140:941-949 https://doi.org/10.1210/en.140.2.941
  44. Motlik, J. and J. Fulka. 1986. Factors affecting meiotic competence in pig oocytes. Theriogenology 25:87-96 https://doi.org/10.1016/0093-691X(86)90185-8
  45. Motlik, J., N. Grozet and Fulka. 1984. Meiotic competence in vitro of pig oocytes isolated from early antral follicles. J. Reprod. Fertil. 72:323-328 https://doi.org/10.1530/jrf.0.0720323
  46. Narula, A., M. Tanej and S. M. Totey. 1996. Morphological development, cell number, and allocation of cells to trophectoderm and inner cell mass of in vitro fertilized and parthenogenetically developed buffalo embryos: the effect of IGF-I. Mol. Reprod. Dev. 44:343-351 https://doi.org/10.1002/(SICI)1098-2795(199607)44:3<343::AID-MRD8>3.0.CO;2-M
  47. Osterlund, C. and G. Fried. 2000. TGFbeta receptor types I and II and the substrate proteins Smad 2 and 3 are present in human oocytes. Mol. Hum. Reprod. 6:498-503 https://doi.org/10.1093/molehr/6.6.498
  48. Paria, B. C., S. K. Das, R. A. Mead and S. K. Dey. 1994. Expression of epidermal growth factor receptor in the preimplantation uterus and blastocyst of the western spotted skunk. Biol. Reprod. 51:205-213 https://doi.org/10.1095/biolreprod51.2.205
  49. Park, Y. S. and Y. C. Lin. 1993. Effect of epidermal growth factor and defined simple media on in vitro bovine oocyte maturation and early embryonic development. Theriogenology 39:475-484 https://doi.org/10.1016/0093-691X(93)90390-Q
  50. Schell, D. L., P. A. Mavrogianis, A. T. Fazleabas and H. G. Verhage. 1994. Epidermal growth factor, transforming growth factor-alpha, and epidermal growth factor receptor localization in the baboon (Papioanubis) oviduct during steroid treatment and the menstrual cycle. J. Soc. Gynecol. Investig. 1:269-276 https://doi.org/10.1177/107155769400100405
  51. Schmidt, A., R. Einspanier, W.Amselgruber, F. Sinowatz and D. Schams. 1994. Expression of insulin-like growth factor I (IGFI) in the bovine oviduct during the oestrous cycle. Exp. Clin. Endocrinol. 102:364-369 https://doi.org/10.1055/s-0029-1211305
  52. Shiraga, M., S. Takahashi, T. Miyahke, S. Takeuchi and H. Fukamachi. 1997. Insulin-like growth factor-I stimulates proliferation of mouse uterine epithelial cells in primary culture. Proc. Soc. Exp. Biol. Med. 215:412-417 https://doi.org/10.3181/00379727-215-44152
  53. Simmen, R. C. M., Y. Ko and F. A. Simmen. 1993. Insulin-like growth factors and blastocyst development. Theriogenology 39:163-175 https://doi.org/10.1016/0093-691X(93)90031-Y
  54. Sirisathien, S., H. J. Hernandez-Foneseca and B. G. Brackett. 2003. Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst developmemt in vitro. Anim. Reprod. Sci. 77:21-32 https://doi.org/10.1016/S0378-4320(02)00272-5
  55. Spanos, S., D. L. Becker, R. M. Winston and K. Hardy. 2000. Anti-apoptotic action of insulin-like growth factor-I during human preimplantation embryo development. Biol. Reprod. 63:1413-1420 https://doi.org/10.1095/biolreprod63.5.1413
  56. Spier, L. J. and S. E. Echternkamp. 1995. The ovarianinsulin-like growth factor system with an emphasis on domestic animals. Domest. Anim. Endocrinol. 12:223-245 https://doi.org/10.1016/0739-7240(95)00021-6
  57. Uhm, S. J., M. K. Gupta, H. J. Chung, J. H. Kim, C. K. Park and H. T. Lee. 2009. Relationship between developmental ability and cell number of day 2 porcine embryo produced by parthenogenesis or somatic cell nuclear transfer. Asian-Aust. J. Anim. Sci. 22:483-491 https://doi.org/10.5713/ajas.2009.80362
  58. Wang, W. H., L. R. Abedeera, T. C. Cantley and B. N. Day. 1997. Effect of oocyte maturation media on development of pig embryos or produced by in vitro fertilization. J. Reprod. Fertil. 111:101-108 https://doi.org/10.1530/jrf.0.1110101
  59. Watson, A. J., M. E. Westhusin and Q. A. Winger. 1999. IGF paracine and autocirne interactions between conceptus and oviduct. J. Reprod. Fertil. Suppl. 54:303-315
  60. Wei, Z., K. W. Park, B. N. Day and R. S. Prather. 2001. Effect of epidermal growth factor on preimplantation development and its receptor expression in porcine embryos. Mol.Reprod. Dev. 60:457-462 https://doi.org/10.1002/mrd.1110
  61. Werb, Z. 1990. Expression of EGF and TGF-$\alpha$ genes in early mammalian development. Mol. Reprod. Dev. 27:10-15 https://doi.org/10.1002/mrd.1080270105
  62. Wood, S. A. and P. L. Kaye. 1989. Effects of epidermal growth factor on preimplantation mouse embryo. J. Reprod. Fertil. 85:575-582 https://doi.org/10.1530/jrf.0.0850575
  63. Xu, X. J., Q. Z. Yang, R. C. Qian and Z. Nakahara. 1992. Development of in vitro fertilized bovine ova in two co-culture systems. Anim. Reprod. 1:372-374
  64. Yoon, S. Y., S. Y. Lee, H. T. Cheong, B. K. Yang, C. I. Kim and C. K. Park. 2003. Effects of growth and hexoses on in vitro development in porcine embryos. Korean J. Anim. Reprod. 27:249-258
  65. Yoshika, K., C. Suzuki, A.Tanaka, I. M. Anas and S. Iwamura. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-119 https://doi.org/10.1095/biolreprod66.1.112