Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Differential Subcellular Localization of Ribosomal Protein L7 Paralogs in Saccharomyces cerevisiae

  • Kim, Tae-Youl (School of Biological Sciences, and Research Center for Functional Cellulomics, Institute of Microbiology, Seoul National University) ;
  • Ha, Cheol Woong (School of Biological Sciences, and Research Center for Functional Cellulomics, Institute of Microbiology, Seoul National University) ;
  • Huh, Won-Ki (School of Biological Sciences, and Research Center for Functional Cellulomics, Institute of Microbiology, Seoul National University)
  • Received : 2008.11.24
  • Accepted : 2009.03.24
  • Published : 2009.05.31
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Abstract

In Saccharomyces cerevisiae, ribosomal protein L7, one of the ~46 ribosomal proteins of the 60S subunit, is encoded by paralogous RPL7A and RPL7B genes. The amino acid sequence identity between RPl7a and RPl7b is 97 percent; they differ by only 5 amino acid residues. Interestingly, despite the high sequence homology, Rpl7b is detected in both the cytoplasm and the nucleolus, whereas Rpl7a is detected exclusively in the cytoplasm. A site-directed mutagenesis experiment revealed that the change in the amino acid sequence of Rpl7b does not influence its subcellular localization. In addition, introns of RPL7A and RPL7B did not affect the subcellular localization of Rpl7a and Rpl7b. Remarkably, Rpl7b was detected exclusively in the cytoplasm in rpl7a knockout mutant, and overexpression of Rpl7a resulted in its accumulation in the nucleolus, indicating that the subcellular localization of Rpl7a and Rpl7b is influenced by the intracellular level of Rpl7a. Rpl7b showed a wide range of localization patterns, from exclusively cytoplasmic to exclusively nucleolar, in knockout mutants for some rRNA-processing factors, nuclear pore proteins, and large ribosomal subunit assembly factors. Rpl7a, however, was detected exclusively in the cytoplasm in these mutants. Taken together, these results suggest that although Rpl7a and Rpl7b are paralogous and functionally replaceable with each other, their precise physiological roles may not be identical.

Keywords

Acknowledgement

Supported by : Korea Research Foundation, Ministry of Education, Science and Technology

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