Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Antioxidative Role of Selenoprotein W in Oxidant-Induced Mouse Embryonic Neuronal Cell Death

  • Chung, Youn Wook (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Jeong, Daewon (Department of Microbiology and Aging-associated Disease Research Center, Yeungnam University College of Medicine) ;
  • Noh, Ok Jeong (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Park, Yong Hwan (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Kang, Soo Im (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Min Goo (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Tae-Hoon (School of Dentistry, Dental Science Research Institute, The 2nd Stage of Brain Korea 21 for the Dental School, Chonnam National University) ;
  • Yim, Moon Bin (Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health) ;
  • Kim, Ick Young (Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University)
  • Received : 2009.02.23
  • Accepted : 2009.03.06
  • Published : 2009.05.31
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Abstract

It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of $H_2O_2$ than control cells. TUNEL assays revealed that $H_2O_2$-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

Keywords

Acknowledgement

Supported by : Korea Research Foundation

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