Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

  • Roh, Seong-Soo (Department of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Hyeung-Sik (Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University) ;
  • Ku, Sae-Kwang (Development Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
  • Published : 2009.12.01
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Abstract

The genotoxic effects of DHU001, a polyherbal formula were evaluated using the mouse micronucleus test. DHU001 was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppression. The results indicats that DHU001 showed no genotoxicity effects up to 2000 mg/kg dosing levels and did not influenced on the total white blood cells and differential counts. In addition, it is also considered that there were no problems from cytotoxicity of DHU001 tested in this study because the polychromatic erythrocyte ratio was detected as > 0.41 in all tested groups.

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References

  1. Aimbire, F., Penna, S.C., Rodrigues, M., Rodrigues, K.C., Lopes-Martins, R.A. and Sertie, J.A. (2007). Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation. Prostaglandins Leukot Essent Fatty Acids, 77, 129-138 https://doi.org/10.1016/j.plefa.2007.08.008
  2. Ashby, J. (1985). Is there a continuing role for the intraperitoneal injection route of exposure in short-term rodent genotoxicity assays?, Mutat. Res., 156, 239-243 https://doi.org/10.1016/0165-1218(85)90069-2
  3. Back, YD., Lee, H.S. and Ku, SK (2008). Effects of DHU001, a mixed herbal formula on acute inflammation in mice. Toxicol. Res., 24, 189-194 https://doi.org/10.5487/TR.2008.24.3.189
  4. Dourish, C.T. (1987). Effects of drugs on spontaneous motor activity in experimental psychopharmacology (Greenshaw, A.J. and Dourish, C.T., Eds.), Humana Press, Clifton, pp. 325-334
  5. EI-Bayoumy, K. (2001). The protective role of selenium on genetic damage and on cancer, Mutat. Res., 475, 123-139 https://doi.org/10.1016/S0027-5107(01)00075-6
  6. Ghayur, M.N., Gilani, A.H. and Janssen, L.J. (2008). Ginger attenuates acetylcholine-induced contraction and $Ca^{2+}$ signalling in murine airway smooth muscle cells. Can. J. Physiol. Pharmacol., 86, 264-271 https://doi.org/10.1139/Y08-030
  7. Grochow, L.B. (1996). Covalent-DNA binding drugs in The chemotherapy source book (Perry, M.C., Ed.), Williams & Wilkins, Baltimore, pp. 293-316
  8. Heddle, J.A. (1973). A rapid in vivo test for chromosome damage, Mutat. Res., 18,187-190 https://doi.org/10.1016/0027-5107(73)90035-3
  9. Heddle, J.A., Hite, M., Kirkhart, B., Mavournin, K., MacGregor, JT, Newell, G.W and Salamone, M.F. (1983). The induction of micronuclei as a measure of genotoxicity: a report of the U.S. environmental protection agency genetox program, Mutat. Res., 123, 61-118 https://doi.org/10.1016/0165-1110(83)90047-7
  10. Heddle, J.A., Stuart, E. and Salamone, M.F. (1984). The bone marrow micronucleus test in Handbook of mutagenicity test procedures (Kilbey, B.J., Legator, M., Nichols, Wand Ramel, C., Eds.), Elsevier, Amsterdam, pp. 441-457
  11. Kalantari, H., Larki, A and Latifi, S.M. (2007). The genotoxicity study of garlic and pasipy herbal drops by peripheral blood micronucleus test. Acta Physiol. Hung., 94, 261-266 https://doi.org/10.1556/APhysiol.94.2007.3.10
  12. Kim, S.S., Lee, S.C., Shin, H.D., Shin, MK, Kim, J.H. and Song, H.J. (2004). Studies on allergy asthma effect of Radix Platicodi. Kor: J. Herbology, 19, 61-70
  13. Korea Food and Drug Administration. (2005). Testing Guidelines for Safety Evaluation of Drugs (Notification No. 2005-60, issued by the Korea Food and Drug Administration on October 21, 2005)
  14. Lee, J.E., Kim, H.J., Choi, E.K., Chai, H.Y., Yun, YW, Kim, D.J., Nam, S.Y., Lee, B.J., Ahn, B.W, Kang, H.G. and Kim, YB. (2003). Four-week repeated-dose toxicity study on Pinellia Extract. Korean J. Lab. Anim. Sci., 19, 127-141
  15. Matter, B. and Schmid, W (1971). Trenimon-induced chromosomal damage in bone-marrow cells of six mammalian species, evaluated by the micronucleus test, Mutat. Res., 12, 417-425 https://doi.org/10.1016/0027-5107(71)90092-3
  16. Miyauchi, A., Hiramine, C., Tanaka, S. and Hojo, K. (1990). Differential effects of a single dose of cyclophosphamide on T cell subsets of the thymus and spleen in mice: flow cytofluorometry analysis, Tohoku J. Exp. Med., 162, 147-167 https://doi.org/10.1620/tjem.162.147
  17. Narimanian, M., Badalyan, M., Panosyan, V, Gabrielyan, E., Panossian, A., Wikman, G. and Wagner, H. (2005). Impact of Chisan (ADAPT-232) on the quality-of-life and its efficacy as an adjuvant in the treatment of acute nonspecific pneumonia. Phytomedicine, 12, 723-729 https://doi.org/10.1016/j.phymed.2004.11.004
  18. Organization for Economic Co-Operation and Development (Ed.). (1997). Guideline for the Testing of Chemicals TG No. 474. Mammalian Erythrocyte Micronucleus Test, July 21,1997
  19. Irwin, S. (1968). Comprehensive observational assessment: la. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology, 13, 222-257 https://doi.org/10.1007/BF00401402
  20. Park, J.H. and Geon, D.G. (2003). Pharmacognostical studies on the Chinese crude drug 'Maig Moon Dong'. Kor. J. Pharmacogn., 34, 6-9
  21. Renner, H.W. (1990). In vivo effects of single or combined dietary antimutagens on mutagen-induced chromosomal aberrations, Mutat. Res., 244, 185-188 https://doi.org/10.1016/0165-7992(90)90070-Z
  22. Rhyu, M.R., Kim, E.Y., Yoon, BK, Lee, YJ. and Chen, S.N. (2006). Aqueous extract of Schizandra chinensis fruit causes endothelium-dependent and -independent relaxation of isolated rat thoracic aorta. Phytomedicine, 13, 651-657 https://doi.org/10.1016/j.phymed.2006.02.003
  23. Schimid, W. (1975). The Micronucleus Test, Mutat. Res., 31, 9-15 https://doi.org/10.1016/0165-1161(75)90058-8
  24. Shin, T.Y. (2003). Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol. Immunotoxicol., 25, 273-283 https://doi.org/10.1081/IPH-120020475
  25. Sun, HX and Pan, H.J. (2006). Immunological adjuvant effect of Glycynhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine, 24,1914-1920 https://doi.org/10.1016/j.vaccine.2005.10.040
  26. Von Ledebur, M. and Schmid, W (1973). The micronucleus test - methodological aspects, Mutat. Res., 19, 109-117 https://doi.org/10.1016/0027-5107(73)90118-8

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