Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Ganglioside GT1b Mediates Neuronal Differentiation of Mouse Embryonic Stem Cells

  • Lee, So-Dam (Dept. of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Jin, Jung-Woo (Dept. of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Choi, Jin (Dept. of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Choo, Young-Kug (Dept. of Biological Science, College of Natural Sciences, Wonkwang University)
  • Published : 2009.09.30
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Abstract

It has been reported that ganglioside GT1b is expressed during neuronal cell differentiation from undifferentiated mouse embryonic stem cells (mESCs), which suggests that ganglioside GT1b has a direct effect on neuronal cell differentiation. Therefore, this study was conducted to evaluate the effect of exogenous addition of ganglioside GT1b to an in vitro model of neuronal cell differentiation from undifferentiated mESCs. The results revealed that a significant increase in the expression of ganglioside GT1b occurred during neuronal differentiation of undifferentiated mESCs. Next, we evaluated the effect of retinoic acid (RA) on GT1b-treated undifferentiated mESCs, which was found to lead to increased neuronal differentiation. Taken together, the results of this study suggest that ganglioside GT1b plays a crucial role in neuronal differentiation of mESCs.

Keywords

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