The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Bacterial Blight Resistance Using SNP and STS Marker-assisted Selection in Aromatic Rice Germplasm

  • Kim, Jeong-Soon (National Agrobiodiversity Center, Rural Development Administration) ;
  • Gwang, Jae-Gyun (National Agrobiodiversity Center, Rural Development Administration) ;
  • Park, Ki-Hun (National Agrobiodiversity Center, Rural Development Administration) ;
  • Shim, Chang-Ki (National Agrobiodiversity Center, Rural Development Administration)
  • Published : 2009.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A molecular survey was conducted to identify the presence of the bacterial blight resistance genes (Xa1, Xa4, xa5, xa13 and Xa21) in 86 accessions of aromatic rice obtained from germplasm. The results revealed that the resistance gene Xa4 (32.5%), Xa21 (17%), and xa5 (16%) were widely observed in tested rice germplasm. Among tested rice germplasm, 49 accessions showed the presence of more than one of five R genes, and 37 accessions possessed none of the R gene. TALLi and 05-IRRi-M-46 showed the presence of Xa4, xa5, xa13 and Xa21. Rice race $415{\times}Ir352$ exhibited positive amplicon for the Xa1, Xa4, xa5 and Xa21. Hyangmibyeo1hos, Ir841-85-1-1-2 and Jasmine85 showed the positive amplicon for the Xa1, Xa4 and xa5 genes. Yekywin Yinkya Hmwe and Khao Dawk Mali105 showed the presence of Xa1, Xa4 and Xa21 gene. Masino Basmati showed the presence of xa5, xa13, Xa21 genes. Xa1 and Xa21 genes were noticed in Mihayngbyeo, Tarana Deshi, Mayataung and AZUCENA. Hyangmibyeo2ho, Basmati 6311 and Basmati405 possessed only two R genes such as Xa4 and xa5, and xa5 and xa13, respectively. The evaluation results of bacterial blight resistance genes in aromatic rice germplasm will help in breeding of multi disease resistant varieties.

Keywords

References

  1. Adhikari, T. B., Mew, T. W. and Leach, J. E. 1999. Genotypic and pathotypic diversity in Xanthomonas oryzae pv. oryzae in Nepal. Phytopathology 89:687-694 https://doi.org/10.1094/PHYTO.1999.89.8.687
  2. Adhikari, T. B., Vera Cruz, C. M., Zhang, Q., Nelson, R. J., Skinner, D. Z., Mew, T. W. and Leach, J. E. 1995. Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Appl. Environ Microbiol. 61:966-971
  3. Bai, J., Choi, S. H., Ponciano, G, Leung, H. and Leach, J. E. 2000. Xanthomonas oryzae pv. oryzae avirulence genes contribute differently and specifically to pathogen aggressiveness. Mol. Plant-Microbe Interact. 13:1322-1329 https://doi.org/10.1094/MPMI.2000.13.12.1322
  4. Chen, H., Wang, S. and Zhang, Q. 2002. New gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63, an elite restorer line. Phytopathology 92:750-754 https://doi.org/10.1094/PHYTO.2002.92.7.750
  5. Chen, X., Ternnykh, S., Xu, Y., Cho, G. and McCouch, S. R. 1997. Development of microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor. Appl. Genet. 95:553-567 https://doi.org/10.1007/s001220050596
  6. Choi, Y. G., Kim, M. K., Jung, K. H., Cho, S. Y., Moon, H. P., Jun B. T., Choi, H. C., Park, N. G, Kim, G. W., Hwang, K. H., Kim, Y. S., Park, R. K. and Cho, J. Y. 1995 An aromatic semi-dwarf lodging resistant rice variety 'Hyangmibyeolho'. Agricultural Science Reports of RDA. Korea. 37:67-74
  7. Chu, Z., Fu, B., Li, Z., Zhang, Q. and Wang, S. 2006. Targeting recessive rice gene, xa13, for bacterial blight resistance to a 14.8-kb DNA fragment. Theor. Appl. Genet. 112:455-461 https://doi.org/10.1007/s00122-005-0145-6
  8. Ezuka, A., Horino, O., Toriyama, K., Shinoda, H. and Morinaka, T. 1974. Inheritance of resistance of rice variety WaseAikoku3 to Xanthomonas oryzae pv. oryzae. Bull. Tokai-kinki, Nat. Agr. Exp. Sta. 28:124-130
  9. Ezuka, A. and Kaku, H. 2000. A historical review of bacterial blight resistance in rice. Bull. Natl. Inst. Agr. Resour. 15:1-207
  10. Finckh, M. R. and Nelson, R. J. 1999. Phylogenetic and patho-typic analysis of rice bacterial blight race 3. Eur. J. Plant Pathol. 105:743-751 https://doi.org/10.1023/A:1008731610813
  11. George, M. L. C., Bustamam, M., Cruz, W. T., Leach, J. E. and Nelson, R. J. 1997. Movement of Xanthomonas oryzae pv. oryzae in southeast Asia detected using PCR-based DNA fin-gerprinting, Phytopathology 87:302-309 https://doi.org/10.1094/PHYTO.1997.87.3.302
  12. Gu, K., Tian, D., Yang, F., Wu, L., Sreekala, C., Wang, D., Wang, G. L. and Yin, Z. 2004. High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in rice, Oryzae sativa L. Theor. Appl. Genet. 108:800-807 https://doi.org/10.1007/s00122-003-1491-x
  13. Gu, K., Yang, B., Tian, D., Wu, L., Wang, D., Sreekala, C., Yang, F., Chu, Z., Wang, G. L., White, F. F. and Yin, Z. 2005. R gene expression induced by type-III effector triggers disease resistance in rice. Nature 435:1122-1125 https://doi.org/10.1038/nature03630
  14. Ha, K. Y., Lee, J. K., Shin, H. T., Lee, S. Y., Yang, B. G., Kim, B. K., Joung, J. I., Kim, Y. D., Shin, M. S., Ko, J. K., Lee, K. S., Kim, C. H. and Cho, S. Y. 2006. A new aromatic and good grain quality Japonica rice variety 'Hyangnambyeo'. Agricultural Science Reports of RDA, Korea. 38:54-60
  15. Ha, K. Y., Kim, Y. D., Lee, J. K., Shin, H. T., Noh, T. H., Choung, J. I., Shin, M. S., Ko, J. K., Kim, B. K., Lee, Y. T., Lee, S. Y., Kim, H. S., Kang, H. J., Lee, K. S. and Lee, S. Y. 2003. A new aromatic and high-yielding Japonica rice cultivar 'Mihyangbyeo'. Korean J. Breed. 35(4):251-252
  16. Huang, N., Angeles, E. R., Domingo, J., Magpantay, G., Singh, S., Zhang, G., Kumaravadivel, N., Bennett, J. and Khush, G. S. 1997. Pyramiding of bacterial blight resistance genes in rice: Marker-assisted selection using RFLP and PCR. Theor. Appl. Genet. 95:313-320 https://doi.org/10.1007/s001220050565
  17. Ikeda, R., Kush, G. S., Tabien, R. E. and Ogawa, T. 1990. A new resistance gene to bacterial blight from Oryza longistaminata. Jpn. J. Breed (Suppl. 1). 40:280-281
  18. Iyer, A. S. and McCouch, S. R. 2004. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol. Plant Microbe. Interact. 17:1348-1354 https://doi.org/10.1094/MPMI.2004.17.12.1348
  19. Khush, G. S. 1981. Breeding rice for multiple disease and insect resistance. p 220-237. In Rice improvement in China and other Asian countries. international Rice Research Institute, Los Banos, Philippines
  20. Khush, G. S., Bacalangco, E. and Ogawa, T. 1990. A new gene for resistance to bacterial blight from O. Longistaminata. Rice Genet. Newsl. 7:121-122
  21. Kim, J. S., Ahn, S. N., Kang, H. K., Cho, Y. H., Gwag, J. G. and Lee, S. Y. 2008a. Estimation of Physico-chemical Characteristics of domestic aroma rice and foreign aroma rice. Korean J. Crop Sci. 53:203-216
  22. Kim, J. S., Ahn, S. N., Cho, Y. H., Gwag, J. G., Kim, T. S., Lee, J. R. and Lee, S. Y. 2008b. Estimation of agronomic characteristics of domestic aromatic rice germplasm and foreign aromatic rice germplasm in RDA Genebank, Korea Korean J. Crop Sci. 53:261-272
  23. Kihupi, A. N., Angeles, E. R. and Khush, G. S. 2001. Genetic analysis of resistance to bacterial blight, Xanthomonas oryzae pv. oryzae, in rice, Oryza sativa L. Euphytica. 117:39-46 https://doi.org/10.1023/A:1004004623439
  24. Kinoshita, T. 1995. Report of committee on gene symbolization, nomenclature and linkage groups. Rice Genet. Newsl. 12:9-153
  25. Leach, J. E., Rhoads, M. L., Vera Cruz, C. M., White, F. F., Mew, T. W. and Leung, H. 1992. Assessment of genetic diversity and population structure of Xanthomonas oryzae pv. oryzae with repetitive DNA element. Appl. Environ. Microbiol. 58:2188-2195
  26. Lee, K. S., Rasabandith S., Angeles, E. R. and Khush, G. S. 2003. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology. 93:147-152 https://doi.org/10.1094/PHYTO.2003.93.2.147
  27. Lin, X. H., Luo, L. J., Mei, H. W., Paterson, A. H., Zhao, X. H., Zhong, D. B., Wang, Y. P., Yu, X. Q., Zhu, L., Tabien, R., Stansel, J. W. and Ying, C. S. 1999. A 'defeated' rice resistance gene acts as a QTL against a virulent strain of Xanth-omonas oryzae pv. oryzae. Mol. Gen. Genet. 261:58-63 https://doi.org/10.1007/s004380050941
  28. Lwin, T., Myint, Y. Y., Nwe, K. T. and Thet, K. M. 2007. Evaluation of Myanmar rice germplasms for resistance to bacterial blight. International training-workshop. The conservation and utilization of tropical/subtropical plant genetic resources. pp. 73-89
  29. Melchinger, A. E. 1990. Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed. 104:1-19 https://doi.org/10.1111/j.1439-0523.1990.tb00396.x
  30. Mew, T. W. 1987. Current status and future prospects of research on bacterial blight of rice. Ann. Rev. Phytopathol. 25:359-382 https://doi.org/10.1146/annurev.py.25.090187.002043
  31. Mew, T. W., Vera Cruz, C. M. and Medalla, E. S. 1992. Changes in race frequency of Xanthomonas oryzae pv. oryzae in response to rice cultivars planted in Philippines. Plant Dis. 76:1029-1032 https://doi.org/10.1094/PD-76-1029
  32. Mohan, M., Nair, S., Bhagwat, A., Krishna, T. G., Tano, M., Bhatia, C. R. and Sasaki, T. 1997. Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol. Breed. 3:87-103 https://doi.org/10.1023/A:1009651919792
  33. Moon, H. P., Choi, Y. G., Jeong, G. H., Hwang, G. H., Lee, L. H., Kim, M. G., Cho, S. Y., Jeon, B. T., Kim, J. I., Hwang, H. G., Choi, H. C., Park, R. G. and Kim, Y. S. 1998. A new, multi disease resistant and semi-dwarf aromatic rice cultivar 'Hangmibyeo 2ho'. Agricultural Science Reports of RDA, Korea. 40:34-40
  34. Moon, H. P., Jung, K. K., Choi, Y. G., Lee, J. H., Hwang, H. G., Kim, M. K., Yang, S. J., Hwang, K. H., Song, M. T., Hong, H. C., Shin, Y. S., Kim, H. Y., Ryu, H. Y., Choi, H. C. and Chung, M. N. 2003. A new aromatic and glutinous rice cultivar 'Seolhyangchalbyeo'. Korean J. Breed. 35:241-242
  35. Nayak, D., Bose, L. K., Singh, S. and Nayak, P. 2008. Additive main effects and multiplicative interaction analysis of host-pathogen relationship in rice-bacterial blight pathosystem. Plant Pathol. J. 24:337-351 https://doi.org/10.5423/PPJ.2008.24.3.337
  36. Nelson, R. J., Baraoidan, M. R., Vera Cruz, C. M., Yap, I. V., Leach, J. E., Mew, T. W. and Leung, H. 1994. Relationship bewteen phylogeny and pathotype for the bacterial blight pathogen of rice. Appl. Environ. Microbiol. 60:3275-3283
  37. Noda, T., Du, P. V., Van, E. L., Dinh, H. D. and Kaku, H. 1999. Pathogenicity of Xanthomonas oryzae pv. oryzae in Vietnam. Ann. Phytopathol. Soc. Jpn. 65:293-296 https://doi.org/10.3186/jjphytopath.65.293
  38. Noda, T., Li, C., Li, J., Ochiai, H., Ise, K. and Kaku, H. 2001. Pathogenic diversity of Xanthomonas oryzae pv. oryzae strain from Yunnam province, China. JARQ. 35:97-103 https://doi.org/10.6090/jarq.35.2
  39. Noda, T., Yamamoto, T., Kaku, H. and Horino, O. 1996. Geographical destribution of pathogenic races of Xanthomonas oryzae pv. oryzae in Japan in 1991 and 1993. Ann. Phytopathol. Soc. Jpn. 65:549-553
  40. Noh, T. H., Lee, D. K., Kang, M. H., Shin, M. S., Shim, H. K. and Na S. Y. 2003. Identification of new of Xanthomonas oryzae pv. oryzae (Xoo) in Korea. Phytopathology 93:S66
  41. Ogawa, T. 1993. Methods and strategy for monitoring race distribution and identification of resistance genes to bacterial leaf blight (Xanthomonas oryzae pv. oryzae) in rice. JARQ 27:71-80
  42. Ou, S. H. 1985. Rice disease (2nd edition). Common wealth Mycological Institute 380p
  43. Ramlingam, J., Basharat, H. S. and Zhang, G. 2001. Polymorphism of DNA markers linked to bacterial blight resistance genes in useful rice germplasm. IRRN. 26:23-24
  44. Ronald, P. C., Albano, B., Tabien, R., Abenes, L., Wu, K., McCouch, S. and Tanksley, S. 1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa2I. Mol. Gen. Genet. 236:113-120 https://doi.org/10.1007/BF00279649
  45. Sakaguchi, S. 1967. Linkage studies on the resistance to bacterial leaf blight, Xanthomonas oryzae (Uyeda et Ishihama) Dowson, in Rice. Bull. Natl. Inst. Agr. Sci. Ser. (in Japanese with English sunnnary)
  46. Sanchez, A. C., Brar, D. S., Huang, N., Li, Z. and Khush, G. S. 2000. Sequence tagged site marker-assisted selection for three bacterial blight resistance genes in rice. Crop Science 40:792-797 https://doi.org/10.2135/cropsci2000.403792x
  47. Sanchez, A. C., Hag, L. L., Yang, D., Brar, D. S., Ausubel, F., Khush, G. S., Yano, M., Sasaki, T., Li, Z. and Huang, N. 1999. Genetic and physical mapping of Xa-13 a recessive bacterial blight resistance genes in rice. Theor. Appl. Genet. 98:1022-1028 https://doi.org/10.1007/s001220051163
  48. Shen, Y. and Ronald, P. 2002. Molecular determinations of disease and resistance in interactions of Xanthomonas oryzae pv. oryzae and rice. Microbes Infect. 4:1361-1367 https://doi.org/10.1016/S1286-4579(02)00004-7
  49. Shin, I. C., Lim, Y. T., Ahn, C. B. and Lee, S. B. 2001. An early-maturing, aromatic pigmented and glutinous rice variety 'Heugseongchal'. Korean J. Breed. 32:135-136
  50. Shin, M. S., Noh, T. H., Kim, K. Y., Shin, S. H., Ko, J. K. and Lee, J. K. 2005. Reaction of Korean rice varieties to bacterial blight race, K3a. Korean J. Crop Sci. 50:151-155
  51. Shin, M. S., Shin, H. T., Jun, B. T. and Choi, B. S. 1992. Effects of inoculation of compatible and incompatible bacterial blight races on grain yield and quality of two rice cultivars. Korean J. Breed. 24:264-267
  52. Shin, S. H., Kim, K. Y., Shin, M. S., Ko, J. K. and Kim, C. K. 2007. Identification of rice varieties containing bacterial blight resistance gene Xal by SNP marker (16PFXa). Korean J. Breed Sci. 39:63-69
  53. Shin, W. C., Baek, S. H., Seo, C. S., Kang, H. J., Kim, C. K., Shin, M. S., Lee, G. S., Hahn, J. H. and Kim, H. S. 2006. Improvement of selection efficiency for bacterial blight resistance using SNP marker in rice. J. Plant Biotechnol. 33:309-313 https://doi.org/10.5010/JPB.2006.33.4.309
  54. Singh, G. P., Srivastara, M. K., Singh, R. V. and Singh, R. M. 1977. Variation in quantitative and qualitative losses caused by bacterial blight on rice varieties. Indian Phytopathol. 30:180-185
  55. Singh, K., Vikal, Y., Singh, S., Leung, H., Dhaliwal, H. S. and Khush, G. S. 2002. Mapping of bacterial blight resistance gene xa8 using microsatellite markers. Rice Genet. Newsl. 19:94-97
  56. Singh, S. 2001. Pyramiding three bacterial blight resistance genes (xa5, xa13, Xa2I) using marker assisted selection into indica rice cultivar PR106. Theor. Appl. Genet. 102:1011-1015 https://doi.org/10.1007/s001220000495
  57. Song, W. Y., Wang, G. L., Chen, L. L., Kim, H. S., Pi, L. Y., Holsten, T., Gardner, J., Wang, B., Zhai, W. X., Zhu, L. H., Fauquet, C. and Ronald, P. 1995. A receptor kinase-like protein encode by the rice disease resistance gene, Xa2I. Science. 270:1804-1806 https://doi.org/10.1126/science.270.5243.1804
  58. Song, W. Y., Wang, G. L., Chen, L., Kim, H. S., Holsten, T., Zhai, W. X., Zhu, L., Fauquet, C. and Ronald, P. 1995. The rice disease resistance gene Xa2I, endodes a receptor kinase-like protein. Science. 270:1804-1806 https://doi.org/10.1126/science.270.5243.1804
  59. Sun, X., Cao, Y., Yang., Z., Xu., C., Li, X., Wang, S. and Zhang, Q. 2004. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. Plant J. 37:517-527 https://doi.org/10.1046/j.1365-313X.2003.01976.x
  60. Wei, M. L., Lu, H. Y. and Lu, C. T. 2005. Bioinfomatics collection and statictics of rice disease/pest resistance genomics research. Crop. Environment & Bioinfomatics. 2:296-306 (in Chinese with English sunnnary)
  61. Yang, Z., Sun, X., Wang S. and Zhang, Q. 2003. Genetic and physical mapping of a new gene for bacterial blight resistance in rice. Theor. Appl. Genet. 106:1467-1472 https://doi.org/10.1007/s00122-003-1205-4
  62. Yoshimura, S., Yamanouchi, U., Katayose, Y., Toki, S., Wang, Z. X., Kono, I., Kurata, N., Yano, M., Iwata, N. and Sasaki, T. 1998. Expression of XaI, a bacterial blight resistance gene in rice, is induced by bacterial inoculation. Proceedings of the National Academy of Science, USA. 95:1663-1668 https://doi.org/10.1073/pnas.95.4.1663
  63. Yoshimura, S., Yoshimura, A., Iwata, N., McCouch, S. R., Abebes, M. L., Baraoidan, Mew, T. W. and Nelson, R. J. 1995. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Mol. Breed. 1:375-387 https://doi.org/10.1007/BF01248415
  64. Zhang, Q. and Mew, T. W. 1985. Adult resistance of rice to bacterial blight. Plant Diseases. 69:896-898
  65. Zhang, Q., Lin, S. C. and Zhao, B. Y. 1998. Identification and tagging a new gene for resistance to bacterial blight, Xanthomonas oryzae pv. oryzae from O. rufipogon. Rice Genet. Newsl. 15:138-142

Cited by

  1. Controlling rice bacterial blight in Africa: Needs and prospects vol.159, pp.4, 2012, https://doi.org/10.1016/j.jbiotec.2011.09.020