DOI QR코드

DOI QR Code

Differential Display Analysis of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Identified Induction of Ras-related Nuclear Protein Binding Protein2 (RanBP2) Gene

  • Published : 2009.03.01

Abstract

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. Toxicity and carcinogenicity of TCDD is well established but the molecular mechanism is still poorly understood. Here, we found the noble responsive genes to TCDD using the differential display analysis. Treatment of HepG2 cells with TCDD showed a significantly different mRNA expression pattern from the untreated cells in differential display analysis. The differentially displayed bands were isolated and used as probes in dot blot and Northern blot analyses. Of thirty-five isolated differentially displayed bands, only two bands were confirmed as positive in dot blot and Northern blot analyses. The nucleotides sequences of these clones were analyzed and the search of Genebank database revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 92%) and the other is an unknown gene. RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis and its role as a novel tumor suppressor has been recently proposed. Thus, these results may suggest the clue elucidating the toxic mechanism of TCDD through RanBP2.

Keywords

References

  1. Cho, Y.-J. Meade J.D., Walden, J.C., Chen, X., Guo, Z. and Liang, P. (2001), Multicolor fluorescent differential display, BioTechniques, 30, 562-572
  2. Dawlaty, M.M., Malureanu, L., Jeganathan, K.B., Kao, E., Sustmann, C., Tahk, S., Shuai, K., Grosschedl, R. and van Deursen, J.M. (2008), Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomeraseIIalpha, Cell, 133, 103-115 https://doi.org/10.1016/j.cell.2008.01.045
  3. Fingerhut, M.A., Halperin, W.E., Marlow, D.A., Piacitelli, L.A., Honchar, P.A., Sweeney, M.H., Greife, A.L., Dill, P.A., Steenland, K. and Suruda, A.J. (1991), Cancer mortality in workers exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. N. Engl. J. Med., 324, 212-218 https://doi.org/10.1056/NEJM199101243240402
  4. Flesch-Janys, D., Berger, J., Gurn, P., Manz, A., Nagel, S., Waltsgott, H. and Dwyer, J.H. (1995), Exposure to polychlorinated dioxins and furans (PCDD/F) and mortality in a cohort of workers from a herbicide-producing plant in Hamburg, Federal Republic of Germany, Am. J. Epidemiol., 142, 1165-1175 https://doi.org/10.1093/oxfordjournals.aje.a117575
  5. Gasiewicz, T.A., Henry, E.C. and Collins, L.L. (2008), Expression and activity of aryl hydrocarbon receptors in development and cancer, Crit. Rev. Eukaryot. Gene Expr., 18,279-321 https://doi.org/10.1615/CritRevEukarGeneExpr.v18.i4.10
  6. Geiger, L.E. and Neal, R.A. (1981), Mutagenicity testing of 2,3,7,8 tetrachlordibenzo-p-dioxin in histidine auxotrophs of Salmonella typhimurium, Toxicol. Appl. Pharmacol., 59,125-129 https://doi.org/10.1016/0041-008X(81)90459-2
  7. Haque, M., Francis, J. and Sehgal, I. (2005), Aryl hydrocarbon exposure induces expression of MMP-9 in human prostate cancer cell lines, Cancer Lett., 225, 159-166 https://doi.org/10.1016/j.canlet.2004.11.043
  8. Kester, H.A., van der Leede, B.M., van der Saag, P.T. and van der Burg, B. (1997), Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation, J. Biol. Chem., 272, 16637-16643 https://doi.org/10.1074/jbc.272.26.16637
  9. Knutson, J.C. and Poland, A. (1982), Response of murine epidermis to 2,3,7,8-tetrachlorodibenzo-p-dioxin: interaction of the ah and hr loci, Cell, 30, 225-234 https://doi.org/10.1016/0092-8674(82)90028-9
  10. Kociba, R.J., Keyes, D.G., Beyer, J.E., Carreon, R.M., Wade, C.E., Dittenber, D.A., Kalnins, R.P., Frauson, L.E., Park, C.N., Barnard, S.D., Hummel, R.A. and Humiston, C.G. (1978), Results of a two-year chronic toxicity and oncogenicity study of 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats,lToxicol. Appl. Pharmacol., 46, 279-303 https://doi.org/10.1016/0041-008X(78)90075-3
  11. Kohle, C., Schwarz, M. and Bock, K.W. (2008), Promotion of hepatocarcinogenesis in humans and animal models, Arch. Toxicol., 82, 623-631 https://doi.org/10.1007/s00204-007-0273-7
  12. Liang, P. (2006), From differential display to DNA microarrays-- a personal account, J. Cell Physiol., 209, 653-658 https://doi.org/10.1002/jcp.20794
  13. Liang, P., Averboukh, L. and Pardee, A.B. (1993), Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization, Nucleic Acids Res., 21, 3269-3275 https://doi.org/10.1093/nar/21.14.3269
  14. Liang, P. and Pardee, A.B. (1992), Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction, Science, 257, 967-971 https://doi.org/10.1126/science.1354393
  15. Manz, A., Berger, J., Dwyer, J.H., Flesch-Janys, D., Nagel, S. and Waltsgott, H. (1991), Cancer mortality among workers in chemical plant contaminated with dioxin, Lancet, 338, 959-964 https://doi.org/10.1016/0140-6736(91)91835-I
  16. Mukerjee, D. (1998), Health impact of polychlorinated dibenzop- dioxins: a critical review, J. Air. Waste Manag. Assoc.,48, 157-165 https://doi.org/10.1080/10473289.1998.10463655
  17. Navarro, M.S. and Bachant, J. (2008), RanBP2: a tumor suppressor with a new twist on TopoII, SUMO, and centromeres, Cancer Cell, 13, 293-295 https://doi.org/10.1016/j.ccr.2008.03.011
  18. Neal, R.A., Olson, J.R., Gasiewicz, T.A. and Geiger, L.E.(1982), The toxicokinetics of 2,3,7,8-tetrachlorodibenzo-pdioxin in mammalian systems, Drug Metab. Rev., 13, 355- 385 https://doi.org/10.3109/03602538209029985
  19. Nebert, D.W., Petersen, D.D. and Fornace, A.J. Jr. (1990), Cellular responses to oxidative stress: the [Ah] gene battery as a paradigm, Environ. Health Perspect., 88, 13-25 https://doi.org/10.2307/3431045
  20. Pichler, A., Gast, A., Seeler, J.S., Dejean, A. and Melchior, F.(2002), The nucleoporin RanBP2 has SUMO1 E3 ligaseactivity, Cell, 108, 109-120 https://doi.org/10.1016/S0092-8674(01)00633-X
  21. Poland, A. and Knutson, J.C. (1982), 2,3,7,8-tetrachlorodibenzo- p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity, Annu. Rev. Pharmacol. Toxicol., 22, 517-554 https://doi.org/10.1146/annurev.pa.22.040182.002505
  22. Raunio, H. and Pelkonen, O. (1983), Effect of polycyclic aromatic compounds and phorbol esters on ornithine decarboxylase and aryl hydrocarbon hydroxylase activities in mouse liver, Cancer Res., 43, 782-786
  23. Reyes, H., Reisz-Porszasz, S. and Hankinson, O. (1992), Identification of the Ah receptor nuclear translocator protein (Arnt) as a component of the DNA binding form of the Ah receptor, Science, 256, 1193-1195 https://doi.org/10.1126/science.256.5060.1193
  24. Stein, J. and Liang, P. (2002), Differential display technology: a general guide, Cell Mol. Life Sci., 59, 1235-1240 https://doi.org/10.1007/s00018-002-8501-z
  25. Stein, S. and Liang, P. (2002), Differential display analysis of gene expression in mammals: a p53 story, Cell Mol. LifeSci., 59, 1274-1279 https://doi.org/10.1007/s00018-002-8506-7
  26. Suskind, R.R. (1985), Chloracne, "the hallmark of dioxin intoxication", Scand. J. Work Environ. Health, 11, 165-171 https://doi.org/10.5271/sjweh.2240
  27. Vogeli-Lange, R., Burckert, N., Boller, T. and Wiemken, A. (1996), Rapid selection and classification of positive clones generated by mRNA differential display, Nucleic Acids Res., 24, 1385-1386 https://doi.org/10.1093/nar/24.7.1385
  28. Yokoyama, N., Hayashi, N., Seki, T., Pante, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U., Fukui, M. and Nishimoto, T. (1995), A giant nucleopore protein that binds Ran/TC4. Nature, 376, 184-188 https://doi.org/10.1038/376184a0
  29. Zugerman, C. (1990), Chloracne, Clinical manifestations and etiology, Dermatol. Clin., 8, 209-213