The Journal of the Korea Contents Association (한국콘텐츠학회논문지)

The Korea Contents Association (한국콘텐츠학회)
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The Change of Bone Mineral Density and Bone Strength by Aquatic Exercise and Drynariae Rhizoma on the Osteoporosis-Induced Rats

수중운동과 Drynariae Rhizoma 투여가 골다공증 백서의 골밀도 및 골강도에 미치는 효과

  • Published : 2009.10.28
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Abstract

This study conducted the following experiment to examine effects of bone metabolism on aquatic exercise, Drynariae Rhizoma and aquatic exercise with Drynariae Rhizoma. This experiment was conducted to compare bone strength, bone mineral density, weight, change of femur, osteocalcin, ALP, Ca and P effects by aquatic exercise for 6 weeks, Drynariae Rhizoma and Drynariae Rhizoma for aquatic exercise with 40 SD rats of postoophorectomy osteoporosis and it divided 10 subjects. experiment group (I) is applying postoophorectomy osteoporosis group, (II) is applying aquatic exercise group, (III) is applying Drynariae Rhizoma group and (IV) is applying aquatic exercise with Drynariae Rhizoma group. These result lead us to the conclusion that osteocalcin were showed a statically increase and blood Ca level were showed a statically decrease on other groups compare to group(I). Consequently, aquatic exercise and Drynariae Rhizoma would be lead to increment of bone metabolism on postoophorectomy osteoporosis.

본 연구는 난소적출로 유발된 골다공증 유발 백서에서 골쇄보(Drynariae Rhizoma)와 수중운동이 골다공증에 미치는 영향을 알아보고자 6주간 수중운동과 Drynariae Rhizoma투여를 실시하여 골강도, 골밀도, 혈중 osteocalcin, ALP, Ca과 P의 양을 측정하였다. 실험설계는 실험군 I은 대조군, 실험군 II는 수중운동 그룹, 실험군 III은 수중운동과 Drynariae Rhizoma 10 mg투여군, 실험군 IV는 Drynariae Rhizoma 10mg투여군으로 나누었다. 각 실험군에서 체중은 유의성 있게 증가하였으며, 골강도는 대조군과 비교하여 수중운동과 골쇄보 10mg 투여군과 골쇄보 10mg 투여군에서 유의하게 증가됨을 관찰 할 수 있었고, 골밀도는 대조군과 비교하여 모든 군에서 증가 하였으나 통계학적으로 유의한 차이는 없었다. 골형성 지표중의 하나인 Osteocalcin 측정치는 대조군과 비교하여 모든 군에서 증가하여 통계학적으로 유의한 차이가 있었다. 혈중 ALP 및 P의 변화는 본 실험에서 유의한 차이를 관찰할 수 없었으나, 혈중 Ca은 골쇄보 투여와 수중 운동으로 인하여 유의하게 감소하였다. 결론적으로, 골다공증 백서에 수중운동과 Drynariae Rhizoma 투여는 난소 적출 후 감소된 골밀도와 골강도 증가에 긍정적 영향을 미치는 것으로 생각된다.

Keywords

References

  1. B. E. C. Nordin, J. Aaron, and R. Speed, "Bone formation and resortion as the determinants trabecular bone volume in postmenopausal osteoporosis," Lancet, 2, pp.27-279, 1981.
  2. J. M. Lane, L. Russell, and S. N. Khan, "Osteoporosis," Clin Orthop Relat Res, 372, pp.139-150, 2000. https://doi.org/10.1097/00003086-200003000-00016
  3. M. J. Davicco, N. Gaumet-Meunier, and P. Lebeque, "Endurance training and bone metabolism in middle-aged rats," Mechan Ageing Develop., 109; pp83-96, 1999. https://doi.org/10.1016/S0047-6374(99)00026-3
  4. C. M. Snow-Harter, "Bone health and prevention of osteoporosis in active and athletic women," Review. Clinics in Sports Medicine, Vol.13, No.2, pp 389-404, 1994.
  5. A. Swissa-Sivan, R. Azoury, and M, Statter "The effect of swimming on bone modeling and composition in young adult rats," Calcif Tissue Int, Vol.47, No.3, pp.173-177, 1990. https://doi.org/10.1007/BF02555984
  6. S. C. Trok and V. Douglas, "Arthritis water exercise program evaluation. Arthritis Care Research," Vol.2, No.1, pp.28-30, 1989. https://doi.org/10.1002/anr.1790020108
  7. J. C. Jeong, S. K. Kang, and C. H. Youn, "Inhibition of Drynariae Rhizoma extracts on bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts," International munopharmacology, Vol.3, No.12, pp1685-1697, 2003. https://doi.org/10.1016/j.intimp.2003.08.003
  8. J. C. Jeong, B. T. Lee, and C. H. Yoon, "Effects of Drynariae Rhizoma on the proliferation of human bone cells and the immunomodulatory activity," Pharmacological Research, Vol.51, No.2, pp.125-136, 2005. https://doi.org/10.1016/j.phrs.2004.06.005
  9. 홍시내, 정지천, "골쇄보(骨碎補) 복합제제가 생쥐의 calvarial osteoblast에서 collagen 용해와 골재 흡수에 미치는 영향", 동국대학교 한의학 연구소, 동국한의학연구소 논문집, 2000.
  10. R. D. Wasnich, "Bone mass measurements in diagnosis and assessment of therapy", Am J Med, 91; 54s, 1991.
  11. L. Gurkan, A. Ekland, and K. M. Gautvik, "Bone changes after castration in rats; A model for osteoporosis," Acta Orthopdica Scandinavica, 57, pp.67-70, 1986. https://doi.org/10.3109/17453678608993219
  12. J. Chow, J. H. Tobias, and K. W. Colston, "Estrogen maintains trabecular bone volume in rats not only by suppression of bone resorption but also by stimulation of bone formation," J Clin Invest, 89; 74-78, 1992. https://doi.org/10.1172/JCI115588
  13. 홍시내, 정지천, "골쇄보 (骨碎補) 복합제제가 생쥐의 calvarial osteoblast 에서 collagen 용해와 골재흡수에 미치는 영향", 동국한의학연구소 논문집, 제9권, pp179-191, 2000.
  14. D. N. Kalu, C. C. Liu, and B. H. Arjmandi, "Effect of ovariectomy and estrogen on the serum levels of insulin-like growth factor-I and insulin-like growth factor binding protein-3," Bone and Mineral, 25; pp.135-148, 1994. https://doi.org/10.1016/S0169-6009(08)80255-3
  15. C. A. Frolik, H. U. Bryant, and E. C. Black, "Time-dependent changes in biochemical bone markers and serum cholesterol in ovariectomized rats: Effects of raloxifene HCI, tamoxifen, estrogen and alendronate," Bone, 18, pp.621-627, 1996. https://doi.org/10.1016/8756-3282(96)00085-3
  16. Y. B. Lee, H. J. Lee, and K. S. Kim, "Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats," Biosci Biotechnol Biochem, 68, pp.1040-1045, 2004. https://doi.org/10.1271/bbb.68.1040
  17. A. E. Grigoriadis, J. N. Heersche, and J. E. Aubin, "Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone," J Cell BioI, 106, pp.2139-2151, 1989.
  18. R. Okasaki, D. Inoue, M. Shibata, and M. Saika, "Estrogen promotes early osteoblast differentiation and inhibits adipocyte differentiation in mouse bone marrow stromal cell lines that express estrogen receptor(ER) ${\alpha}\;or\;{\beta}$," Endocrinology, 143, pp.2349-2356, 2002. https://doi.org/10.1210/en.143.6.2349
  19. 김남현, 최종혁, 이환모, "흰쥐의 난소제거 후 발생한 골다공증에 대한 홍삼 사포닌의 효능", 대한정형외과학회지, 제30권, 제4호, pp.808-816, 1995.
  20. 홍희옥, 이준혜, 정동춘, "운동 형태가 흰쥐의 뼈 형성에 미치는 영향", 한국영학회지, 제34권, 제5호, pp.541-546, 2001.
  21. A. Simkin, I. Leichter, and A. Swissa, "The effect of swimming activity on bone architecture in growing rats," J Biomech, 22, pp.845-851, 1989. https://doi.org/10.1016/0021-9290(89)90068-7
  22. C. C. Johnston Jr, S. L. Hui, and R. M. Witt,"Early menopausal changes in bone mass and sex steroids," J Clin Endocrinol Metab, 61; 905-911, 1985. https://doi.org/10.1210/jcem-61-5-905
  23. 오한진, 오장균, 이동배, "폐경 후 골다공증의 치료방법별 효과 및 골대사 지표 변화에 의한 골밀도 변화의 예측성", 가정의학회지, 제 22권, 제8호, pp.1234-1246, 2001.
  24. R. J. Duda, J. F. O'Brien, and J. A. Katzmann. "Concurrent assays of circulating bone-glaprotein and alkaline phophatase: Effects of sex, age, and metabolic bone disease," J Clin Endocrinol metab, 66; pp.951-957, 1988. https://doi.org/10.1210/jcem-66-5-951
  25. A. A. Bouman. P. G. Scheffer, and M. E. Ooms, "Two bone alkaline phosphatase assays compated with osteocalcin as a marker of bone formation in healthy elderly women," Clin. Chem, 41; pp.196-199, 1995.
  26. S. A. Bowles, Kurdy and N, Davis AM, "Serum osteocalcin, total and bone-specific alkaline phosphatase following isolated tibial shaft fracture," Ann Clin Biochem, 33; pp.196-200, 1996. https://doi.org/10.1177/000456329603300304
  27. S. E. Papapoulos, M. Frolich, and A. H. Mudde,"Serum osteocalcin in Paget's disease of bone: Basal concenrations and response to biosphsphonate treatment," J Clin Endocrinol Metab, 65, pp.89-94, 1987. https://doi.org/10.1210/jcem-65-1-89
  28. R. C. Watson, H. Grossman, and M. A. Meyers, "Radiologic findings in nutritional disturbances, In: Shi ME, Olson JA, Shike M. Modern nutrition in health and disease," 8th ed. Philadelphia: Lea and Febiger, pp.861-908, 1994.
  29. 김남수, 유우순, 강창원, "한쥐에서 난소제거술로 유발시킨 골다공증시 steocalcin, bone-specific alkaline phosphatase, estrogen, IGF-I, Ca2+, P와 bone density의 변화", 대한수의학회지, 제40권, 제4호, pp.755-762, 2000.