Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Rubus coreanus Extracts on Ultraviolet-A Irradiated Cultured Human Skin Fibroblasts

자외선이 조사된 인간피부섬유아세포에 복분자 추출물이 미치는 영향

  • Jeong, Hyang-Suk (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Ha, Ji-Hye (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Kim, Young (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Oh, Sung-Ho (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Kim, Seoung-Seop (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Jeong, Myoung-Hoon (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Lee, Hyeon-Yong (College of Bioscience & Biotechnology, Kangwon National Univ.)
  • 정향숙 (강원대학교 BT특성화학부대학) ;
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 김영 (강원대학교 BT특성화학부대학) ;
  • 오성호 (강원대학교 BT특성화학부대학) ;
  • 김승섭 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Published : 2009.10.30
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Abstract

Sunlight, and in particular its UV component, is the major environmental trigger that underlies the major signs of human skin and skin cancer in general. Therefore, this study was carried out to investigate the UV protection effects of R. coreanus. R. coreanus was extracted by ultra high pressure extraction process at 500 MPa and $30^{\circ}C$ for 5 and 15 minutes. The cytotoxicity of the extracts extracted by ultra high pressure process on human dermal fibroblast cell CCD-986sk, human kidney normal cell HEK293, and human lung normal cell HEL299 was measured as 17.5%, 16.5% and 14.0%, respectively in adding $1.0\;mg/m{\ell}$ of the samples, which was much lower than that from conventional water extraction method at $100^{\circ}C$ as 23.2%, 22.5%, 21.2%. The secretion of $NO^-$ from macrophage showed $15.9\;{\mu}M$ on the R. coreanus extract from this process, which was higher than others. Prostaglandin $E_2$ ($PGE_2$) production from UV-induced human skin cells was also greatly decreased down to $510\;pg/m{\ell}$, compared to the control. From the results, we considered that the extracts from R. coreanus could be potent natural materials for skin anti-inflammation agent, and could be used as a potential anti-aging for the photo-damaged skin.

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References

  1. Bae GH. (2000). The medicinal plants of Korea. Kyohak Publishing Co., Ktd. Seoul, Korea. p. 231-231
  2. Cho KH, Lee MK, Jo SJ, Kim KH, Park KC, Eun HC and Chung JH. (2003). Histologic changes in the skin with photoaging. Korean Dermatological Association. 41:754-760
  3. Deliza R, Rosenthal A, Abadio FBD, Silva CHO and Castillo C. (2005). Application of high pressure technology in the fruit juice processing: benefits perceived by consumers. Journal of Food Engineering. 67:241-246 https://doi.org/10.1016/j.jfoodeng.2004.05.068
  4. Dol R and Peto R. (1981). The causes of cancer; quantitative estimates of avoidable risks of cancer in the Unitede States today. Journal of the National Cancer Institute. 66:1192-1308 https://doi.org/10.1093/jnci/66.6.1192
  5. Gilchrest BA. (1989). Skin aging and photoaging. Journal of the American Academy of Dermatology. 21:610-613 https://doi.org/10.1016/S0190-9622(89)70227-9
  6. Kwon MC, Kim CH, Na CS, Kwak HG, Kim JC and Lee HY. (2007). Comparison of immuno-modulatory regulatory activities of Rubus coreanus Miquel by ultra high pressure extracts process. Korean Journal of Medical Crop Science. 15:398-404
  7. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH, and Lee HY. (2007). Toxicity reduction and improvement of anticancer activities from Rhodiola sacha-linensis A. Bor by ultra high pressure extracts process. Korean Journal of Medical Crop Science. 15:411-416
  8. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, Park UY and Lee HY. (2008). Skin-Whitening and UVprotective effects of Angelica gigas Nakai extracts on uitra high pressure extraction process. Korean Journal of Medicinal Crop Science. 16:255-260
  9. Kim SD, Lee BH, Sohn DW, Cho YH, Lee SM, Kim JO and Kim SW. (2008). The effect of herbal formulation KH-305 mainly consisted of Rubus Coreanus on benign prostatic hyperplasia-induced rat. The Korean Society of Pharmacognosy. 39:80-85
  10. Park JH, Oh SM, Lim SS, Lee YS, Shin HK, Oh YS, Choe NH, Park JH and Kim JK. (2006). Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages. Biochemical and Biophysical Research Communication. 351: 146-152 https://doi.org/10.1016/j.bbrc.2006.10.008
  11. Yoo OK, Back HI and Cha YS. (2008). Quality characteristics of pudding added with bokbunja (Rubus coreanus Miquel) fruit juice and bokbunia wine. Korean Journal of Food Culture. 23:616-620
  12. Yoon DH, Kim HO, Kim TY, Kim JW and Song KY. (1995). The effect of ultraviolet B irradiation on the skin barrier function in hairless mice. Korean Dermatological Association. 33:669-678
  13. Yang HM, Lim SS, Lee YS, Shin HK, Oh YS and Kim JK. (2007). Comparison of the anti-inflammatory effects of the extracts from Rubus coreanus and Rubus occidentalis. Korean Journal of Food Science and Technology. 39:342-347