Prevalence of M. paratuberculosis antibody in dairy cattle in Seosan-Taean areas for M.R.T. samples

충남 서산.태안지역에서 착유증인 젖소의 M.R.T.용 집합유에 대한 요네병 감염률 조사

  • Jeon, Dong-Min (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Yook, Sim-Yong (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Nam, I-Hyun (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Lee, Mi-Sung (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Han, Woo-Soo (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Kang, Hyeong-Joo (Taean branch of the Chungnam Veterinary Research Institute) ;
  • Lee, Jea-Bong (Taean branch of the Chungnam Veterinary Research Institute)
  • 전동민 (충청남도 가축위생연구소 태안지소) ;
  • 육심용 (충청남도 가축위생연구소 태안지소) ;
  • 남이현 (충청남도 가축위생연구소 태안지소) ;
  • 이미성 (충청남도 가축위생연구소 태안지소) ;
  • 한우수 (충청남도 가축위생연구소 태안지소) ;
  • 강형주 (충청남도 가축위생연구소 태안지소) ;
  • 이재봉 (충청남도 가축위생연구소 태안지소)
  • Published : 2009.09.30

Abstract

TThis survey was carried out to investigate the prevalence of the antibody for bovine paratuberculosis (Johne's disease) in dairy cattle in Seosan-Taean area. From February to August in 2009, 254 M.RT. samples were collected from 57 farms in the regions and enzyme immuno-sorbent assay (ELISA) was conducted. Among 254 samples, 13 (5.1%) M.R.T. samples of 3 (5.2%) farms were positive by ELISA. In regional analysis, 1 (3.1%) of 34 farms in Seasan and 2 (8.6%) of 23 farms in Taean were positive in ELISA. According to the raising scale of dairy farms, the farm with below 30 heads showed the higher positive rate (2 out of 3 positive farms) than the farms with over 30 heads (1 out of 3 positive farms).

Keywords

References

  1. 김 두, 전관준, 김종택, 신광순, 신명균, 장국현, 김정기. 2002. 강원지역 젖소의 요네병 감염실태. 대한수의학회지 42(1): 81-88
  2. 김종만, 안종삼, 우승룡, 조동희, 조윤상, 박정문, 윤용덕, 장국현 .1994. 면역학적인 방법에 의한 한우와 유우의 요네병 발생조사. 대한수의학회지 34: 93-97
  3. 박혜연, 원상민, 장지택, 정성진, 문수평, 함유식. 2007. 울산지역 젖소의 요네병 감염실태 조사, 울산광역시 보건환경 연구원보 4: 462-473
  4. 이방환, 임봉호, 하창수, 성홍룡. 1982. 국내 발생의 우 파라결핵병 (Johne' s disease) 례에 대한 임상병리학적 추적조사 보고. 대한수의사회지 19(2): 8-20
  5. 이선미, 김미숙, 장영술, 전령훈, 박노찬.2009. 경북 동부지역 젖소 및 한우의 요네병 감염실태조사 한국가축위생학회지 32(2): 171-176
  6. 전윤성, 이방환, 김종배, 최철순, 김진구. 1984. 우유래 Mycobactin 의존성 항산성 세균(M. paratuberculosis)의 분리동정. 대한수의학회지 24(1): 58-63
  7. Billman-Jacobe H, Carrigan M, Cockram F, Comer LA, Gill IJ, Hill JF, Jessep T, Milner AR, Wood PR. 1992. A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle. Aust Vet J 69(2): 25-28 https://doi.org/10.1111/j.1751-0813.1992.tb07426.x
  8. Colstin A, McConnell I, Bujdso R. 1994. Cloning and expression in Escherichia coli of DNA encoding a 60kDa stress protein of Mycobacterium paratuberculosis, the causative agent of Johne's disease. Microbiology 140: 3329-3336 https://doi.org/10.1099/13500872-140-12-3329
  9. Cox JC, Drane DP, Jonεs SL, Ridge S. Milner AR. 1991. Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne' S disease in cattle. Aust Vet J 68: 157-160 https://doi.org/10.1111/j.1751-0813.1991.tb03167.x
  10. el-Zaatari FA, Naser SA, Engstrand L, Hachem CY, Graham DY. 1994. Identification and charaterization of Mycobacterium paratuberculosis recombinant proteins expressed in E. coli. Curr Microbiol 29: 177-184 https://doi.org/10.1007/BF01570760
  11. Gitnick G, Collins J, Beaman B, Brooks D, Arthur M, Imaeda T, Palisechesky M. 1989. Preliminary report on isolation of mycobacteria from patients with Crohn's disease. Dig Dis Sci 34(6): 925-932 https://doi.org/10.1007/BF01540280
  12. Graham DY, Markesich DC, Yoshmura HH. 1987. Mycobacteria and inglammatory bowel disease. Result of culture. Gastroenterology 92: 436-442
  13. Hendrick S, Duffield T, Leslie K, Lissemore K, Archmbault M, Kelton D. 2005. The prevalence of milk and serum antibodies to Mycobaterium avium subspecies paratuberculosis in dairy herds in Ontario. Can Vet J 46(12): 1126-1129
  14. Johne HA, Frothingham L. 1895. Ein eigenthuemlicher Fall von TuberKulose beim Rind. Deutche Zeitschifr Tiermed Pathol 21: 438-454
  15. Kim D, Park HW. 1999. Experssion of the C-terminal of 34KDa protein of Mycobacterim paratuberculosis. Korean J Vet Res 0: 86-93
  16. McFadden JJ, Butcher PD, Chiodini R, Hermon-Taylor J. 1987. Crohn's disease isolated mycobacteria are identical to Mycobacterium paratuberculosis, as determined by DNA probes that distinguish between mycobacterial species. J Clin Microbiol 25(5): 796-80l
  17. Milner AR, Lepper AW, Symonds WN, Gmner E. 1987. Analysis by ELISA and Wentern blotting of antybody ractivities in cattle infected with Mycobacterium paratuberculosis after absorption of semm with M phlei. Res Vet Sci 42: 140-144
  18. Tsai SJ, Hutchinson LJ, Zarkower A. 1989. Comparison of dot immunobinding assay, enzyme-linked immunodiffusion for serodiagnosis of paratuberculosis. Can J Vet Res 53: 405-410
  19. Twort FW, Ingram GLY. 1912. A method for isolating and cultivating Mycobacterium enteritidis chronicae psudotuberculosae bovis johne and some experiments on the preparation of a diagonostic vaccine for pseudotuberculosae enteritis fo bovines, Proc Royal Soc London 84: 517-543 https://doi.org/10.1098/rspb.1912.0011
  20. VanLeeuween JA, Keefe GP, Tremblay R, Power C, Wichitel JJ. 2001. Seroprevalence of infection with Mycobacterium avium subspecies paratuberculosis, bovine leukemia virus, and bovine viral diarrhea virus in maritime Canada dairy cattle. Can Vet J 42(3): 193-198
  21. VanLeeuween JA, Tiwari A, Plaizier JC, Whiting TL. 2006. Seroprevalences of antibodies against bovine leukemia virus, bovine viral diarrhea virus, Mycobacterium avium subspecies paratuberculosis, and Neospora caninum in beef and cattle in manitoba. Can Vet J 47(8): 783-786
  22. Vary PH. Andersen PR, Green E, Hermon-Taylor J, McFadden JJ. 1990. Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin MicrobioI 28(5): 933-937
  23. Yokornizo Y, Yugi H, Merkal RS. 1985. A method for avoiding false-positive reactions in an enzyme-linked immunosobent assay (ELISA) for the diagnosis of bovine paratuberculosis. Nippon Juigaku Zashi 47: 111-119 https://doi.org/10.1292/jvms1939.47.111