Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Characterization and isolation of shiga toxin-producing Escherichia coli from Bovine feces and Carcass

소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성

  • Chae, Hee-Sun (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Neung-Hee (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Han, Hye-Jin (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Son, Hong-Rak (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Chang-Ki (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Sun-Heung (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Lee, Jung-Hark (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Jong-Taek (College of Veterinary Medicine, Kangwon National University)
  • Published : 2009.09.30
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Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1 [2, 8.4%]), stx2 [3, 12.6%] and stx1 and stx2 [19,87.5%]). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.

Keywords

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