Parasites, Hosts and Diseases

  • 제47권4호
  • /
  • Pages.409-412
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  • 2009
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Antigenemia and Specific IgM and IgG Antibody Responses in Rabbits Infected with Toxoplasma gondii

  • Quan, Juan Hua (Department of Infection Biology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University) ;
  • Hassan, Hassan Ahmed (Department of Infection Biology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University) ;
  • Cha, Guang-Ho (Department of Infection Biology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University) ;
  • Shin, Dae-Whan (Department of Infection Biology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University) ;
  • Lee, Young-Ha (Department of Infection Biology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University)
  • 발행 : 2009.12.31
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초록

In this experiment, the correlation between antigenemia and specific antibody responses in Toxoplasma gondii-infected rabbits was assessed. We injected 1,000 T. gondii tachyzoites (RH) subcutaneously into 5 rabbits. Parasitemia, circulating antigens, and IgM and IgG antibody titers in blood were tested by ELISA and immunoblot. For detection of parasitemia, mice were injected with blood from rabbits infected with T. gondii and mice died between days 2 and 10 post-infection (PI). Circulating antigens were detected early on day 2 PI, and the titers increased from day 4 PI and peaked on day 12 PI. Anti-Toxoplasma IgM antibody titers increased on day 6 PI and peaked on days 14-16 PI. IgG was detected from day 10 PI, and the titers increased continuously during the experiment. The antigenic protein patterns differed during the infection period, and the number of bands increased with ongoing infection by the immunoblot analysis. These result indicated that Toxoplasma circulating antigens during acute toxoplasmosis are closely related to the presence of parasites in blood. Also, the circulating antigen levels were closely correlated with IgM titers, but not with IgG titers. Therefore, co-detection of circulating antigens with IgM antibodies may improve the reliability of the diagnosis of acute toxoplasmosis.

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피인용 문헌

  1. Antigenemia and Specific IgM and IgG Antibody Responses in Rabbits Infected with Toxoplasma gondii vol.47, pp.4, 2009, https://doi.org/10.3347/kjp.2009.47.4.409
  2. Evaluation of Toxoplasma gondii as a live vaccine vector in susceptible and resistant hosts vol.4, pp.None, 2009, https://doi.org/10.1186/1756-3305-4-168
  3. Evaluation of automated assays for immunoglobulin G, M, and A measurements in dog and cat serum vol.42, pp.3, 2013, https://doi.org/10.1111/vcp.12069
  4. Prevalence of Toxoplasma gondii Infection in Rabbits of Korea by Serological Tests and Nested Polymerase Chain Reaction vol.75, pp.12, 2009, https://doi.org/10.1292/jvms.13-0360
  5. The merits of designed ELISA avidity kit in detection of Toxoplasma gondii IgG antibody in laboratory conditions vol.3, pp.1, 2009, https://doi.org/10.4103/2229-5070.113904
  6. Occurrence of Toxoplasma gondii in domestic rabbits of Northeastern Brazil vol.61, pp.3, 2009, https://doi.org/10.1515/ap-2016-0066
  7. Epidemiological and Public Health Significance of Toxoplasma gondii Infection in Wild Rabbits and Hares: 2010-2020 vol.9, pp.3, 2009, https://doi.org/10.3390/microorganisms9030597