Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effect of Swimming and Minocycline on Motor Function and Expression of Bcl-2 after Spinal Cord Injury in the Rats

미노싸이클린과 수영훈련이 척수손상 흰쥐의 운동기능 및 Bcl-2발현에 미치는 영향

  • Ha, Mi-Sook (Department of Physical Therapy, Msan University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Eingineering, Silla University)
  • Published : 2009.10.30
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Abstract

This study was designed to investigate the effects of swimming and minocycline on motor function recovery and Bcl-2 expression after spinal cord injury (SCI) in rats. After operation, neurological motor behavior test (BBB scale) on days 1, 4, 7, 10, and 14 were tested. Western blot and immunohistochemical assessment (Bcl-2) were performed on day 14. BBB scale started to show a statistically significant difference on day 7 (p<0.05). On day 14, it showed the most significant (p<0.05) difference. Expression of Bcl-2 increased in all the experimental groups. In particular, the highest expression of Bcl-2 appeared in the swimming and minocycline groups. Based on these results, minocycline and swimming were the most effective factors in the motor behavior function and immunohistochemical assessment of SCI rats.

본 연구는 척수 앞뿔의 세포손상을 준 흰쥐를 대상으로 수영운동과 미노싸이클린의 치료적 중재를 통해 운동기능과 신경계의 회복을 알아보고 운동에 의한 척수손상의 신경학적 회복기전을 밝히는 것의 목적이 있다. 본 연구의 진행은 Sprague-Dawley계 흰쥐 28마리의 허리뼈 1-2번 사이에 6-OHDA로 척수손상을 일으킨 다음 3일 후에 치료를 시작하였다. 수영은 주 5회 15분간 운동을 실시하고 미노싸이클린은 척수손상을 유발한 후 12시간마다 복강내 주입하였다. 척수손상을 유발한 후 치료를 시작하기 전에 운동기능의 평가를 시행하며 2주 동안 4번의 검사가 이루어졌으며, Bcl-2 발현에 관한 측정은 2주간 치료 후 검사하였다. BBB 척도에서 대조군에 비해 실험군에서 7일에 차이가 있었으며, 14일에는 II, III군에 비해 실험군 Ⅳ군에서 증가하였다. Bcl-2의 면역학적 소견에서 실험군 모두 대조군에 비해 척수 앞뿔에서 Bcl-2의 발현이 증가되었으며, 실험군 Ⅳ에서 가장 많은 Bcl-2의 발현이 관찰되었다. 척수손상 후 미노싸이클린과 수영은 행동학적, 면역학적 소견을 긍정적으로 개선시키는 것으로 나타났으며, 수영과 미노싸이클린을 함께 실시한 경우 세포사멸의 감소와 운동기능의 회복에 효과가 있음을 알 수 있었다.

Keywords

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