Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Increase of the D-chiro-inositol and D-Pinitol Contents by Abiotic Stress in the Buckwheat Seedlings

쓴메밀 유식물에서 비 생물학적 스트레스에 의한 D-chiro-inositol과 D-pinitol의 함량 증가

  • Ahn, Chul-Hyun (Department of Bioscience and Biotechnology, University of Suwon) ;
  • Park, Phun-Bum (Department of Bioscience and Biotechnology, University of Suwon)
  • 안철현 (수원대학교 생명공학과) ;
  • 박훤범 (수원대학교 생명공학과)
  • Published : 2009.10.30
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Abstract

D-chiro-Inositol, which is the isomer of myo-inositol, is a well known drug for the treatment of type II diabetes. The methylated form of D-chiro-inositol, D-pinitol and D-chiro-inositol are synthesized when the plants are exposed to the abiotic stresses such as drought, salinity and low temperature as osmoprotectants. In soybean, myo-inositol is converted to ononitol by O-methyltransferase, and ononitol is converted to D-pinitol by ononitol epimerase and finally converted to D-chiro-inositol by demethylase. However there have been some reports that in buckwheat, myo-inositol can be converted to D-chiro-inositol directly. This study was conducted to determine the changes of soluble cyclitols in buckwheat seedlings after exposure to salt and drought stresses by GC-FID. The results indicated that myo-inositol may be the precursor of D-chiro-inositol biosynthesis.

myo-Inositol의 이성질체인 D-chiro-inositol은 제 2형 당뇨병에 탁월한 효과를 나타내고 있다. D-chiro-inositol의 methylated 형태인 D-pinotol과 D-chiro-inositol은 식물체가 가뭄, 염, 저온과 같은 비 생물학적 스트레스에 노출되게 되면 삼투보호물질로써 합성되어 축적되어 진다. 대두에서는 myo-inositol O-methyltrasnferase에 의하여 ononitol이 합성되고 ononitol epimerase에 의하여 D-pinitol이 합성되고 최종적으로 demethylase에 의하여 D-chiro-inositol이 합성되어 진다. 그러나 쓴메밀에서는 myo-inositol이 직접적으로 D-chiro-inositol로 합성되어 진다는 보고가 있다. 본 실험에서는 쓴메밀에 비 생물학적 스트레스를 처리한 후 cyclitols의 변화를 측정하였다. 그 결과 쓴메밀에서는 myo-inositol epimerase에 의하여 myo-inositol이 D-chiro-inositol로 직접 전환된다는 사실을 확인하였다.

Keywords

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