Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Differences of Garlic Powder Ingestion and Exercise Training on Blood Lipids, MDA and SOD in Rats

운동훈련과 마늘가루 섭취에 의한 흰쥐의 혈중 지질 및 MDA, SOD 활성 차이

  • Jeon, Byung-Duk (Dept. of Sports Welfare, Kyungbuk University of Foreigh Studies) ;
  • Kim, Jin-Ha (Dept. of Leisure Sports, Kyungpook Nat'l University) ;
  • Ryu, Sung-Pil (Dept. of Leisure Sports, Kyungpook Nat'l University)
  • 전병덕 (경북외국어대학교 관광스포츠경영학부) ;
  • 김진하 (경북대학교 생태환경대학 레저스프츠학과) ;
  • 류승필 (경북대학교 생태환경대학 레저스프츠학과)
  • Published : 2009.10.30
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Abstract

This study investigated the effect of exercise training and garlic powder ingestion on blood lipids and antioxidants activity in rats. Twenty-eight male Sprague Dawley rats were fed a high-fat diet with or without garlic powder (500 mg/kg) for four weeks as grouped in control (CON), exercise (EXE), garlic (GAR), and garlic + exercise training (GAREXE), respectively. EXE and GAREXE were trained on the treadmill for the same periods. Weight of fats (mesentery, perirenal, and epididymal) were weighed and blood glucose, triglycerides (TG), total cholesterol (TC), and high density lipoprotein- cholesterol (HDL-C) were analyzed and low density lipoprotein-cholesterol (LDL-C) was calculated. Malondialdehyde (MDA) and superoxide dismutase (SOD) for lipid peroxidation were analyzed in liver tissue. Body weight in GAREXE was significantly lower in the statistics than that in other groups (p<0.05), and the volume of fat in GAR and GAREXE was also much lower (p<0.05). Blood glucose was significantly lower in EXE and GAR (p<0.05), however, there was no effect of exercise training. Blood TG was lower in GAR and GAREXE (p<0.05), however, there was no effect of exercise training. HDL-C was significantly improved in EXE and GAR compared to CON (p<0.05), and GAREXE was higher than EXE (p<0.05). MDA content was considerably lower in GAREXE compared to EXE (p<0.05), and SOD activity was much higher in other groups compared to CON (p<0.05). In addition, GAREXE was significantly higher than EXE and GAR, thus there was significant increase when a garlic diet was carried out together with exercise (p<0.05). These results suggested that garlic powder ingestion during the training periods had a beneficial effect of lowering glucose and enhancing blood lipids profiles. Moreover, it also has antioxidant effects, which means that it could possibly suppress aging. It is necessary to inspect various effects of garlic with a variety of research methods regarding sampling process, production process, intake method, etc.

본 연구는 운동훈련과 마늘가루 섭취가 혈중지질과 항산화활성에 미치는 영향을 조사한 것으로서 28마리의 SD계 수컷 흰쥐를 사용하여 고지방식이와 함께 마늘분(500 mg/kg)을 사료에 혼합시켜 섭취시켰다. 집단은 대조군(CON), 운동군(EXE), 마늘군(GAR), 그리고 마늘분과 운동훈련 병행군(GAREXE)로 각각 구분하였다. EXE와 GAREXE는 트레드밀 달리기를 4주동안 실시하였다. 지방량(장간막, 신주위, 부고환주위)의 정량과 혈중 글루코스, 중성지방(Triglyceride: TG), 총콜레스테롤(Total cholesterol: TC), 고밀도지단백콜레스테롤(High density lipoprotein cholesterol: HDL-C), 저밀도지단백콜레스테롤(Low density lipoprotein cholesterol: LDL-C) 등을 분석하였다. 간조직의 MDA (Malondialdehyde)와 SOD (Superoxide dismutase) 활성을 분석하였다. GAREXE의 체중은 다른 집단에 비하여 통계적으로 유의하게 낮은 것으로 나타났으며(p<0.05), GAR과 GAREXE이 경우에 지방량 역시 유의하게 낮은 것으로 나타났다(p<0.05). 혈중 글루코스는 CON이 다른 집단에 비하여 통계적으로 유의하게 높은 것으로 나타났으며 (p<0.05), TG는 GAR과 GAREXE가 다른 집단에 비하여 유의하게 낮은 것으로 나타났다. TC는 GAREXE가 CON 및 GAR에 비하여 통계적으로 유의하게 낮은 것으로 나타났으며(p<0.05), HDL-C는 CON에 비하여 EXE와 GAR에서 유의하게 개선된 것으로 나타났으며(p<0.05), GAREXE는 EXE에 비하여 유의하게 높은 농도를 나타내었다(p<0.05). MDA 함량은 GAREXE가 EXE에 비하여 유의하게 낮은 것으로 나타났으며(p<0.05), SOD 활성은 CON에 비하여 다른 집단에서 유의하게 높은 것으로 나타났다(p<0.05). GAREXE의 경우에 EXE와 GAR에 비하여 유의하게 높은 것으로 나타나 운동과 병행할 경우의 유의한 상승효과가 나타났다(p<0.05). 이러한 결과를 볼 때, 운동훈련을 실시하는 경우에 마늘분 섭취는 혈중 글루코스를 낮추고, 혈중지질을 개선하는데 효과가 있으며, 항산화효과 역시 나타났다고 생각된다. 추후 마늘의 추출법, 제조공정, 섭취방법 등에 대한 다양한 연구방법적 접근을 통해서 마늘이 가지고 있는 다양한 효과에 대한 검증이 필요하다고 사료된다.

Keywords

References

  1. Abramovitz, D., S. Gavri, D. Harats, H. Levkovitz, D. Mirelman, T. Miron, S. Eilat-Adar, A. Rabinkov, M. Wilchek, M. Eldar, and Z. Vered. 1999. Allicin-induced decrease in formation of fatty streaks (atherosclerosis) in mice fed a cholesterol-rich diet. Caron. Artery Dis. 10, 515-519 https://doi.org/10.1097/00019501-199910000-00012
  2. Ackermann, R. T., C. D. Mulrow, G. Ramirez, C. D. Gardner, 1. Morbidoni, and V. A. Lawrence. 2001. Garlic shows promise for improving some cardiovascular risk factors. Arch. Intern. Med. 161, 813-824 https://doi.org/10.1001/archinte.161.6.813
  3. Ali, M., M. Afzal, R. A. Hassan, A. Farid, and J. F. Burka. 1990. Comparative study of the in vitro synthesis of prostaglandins and thromboxanes in plants belonging to Liliaceae family. Gen. Pharmacol. 21, 273-276 https://doi.org/10.1016/0306-3623(90)90821-3
  4. Ali, M., M. Thomson, and M. Afzal. 2000. Garlic and onions: their effect on eicosanoid metabolism and its clinical relevance. Prostaglandins Leukot. Essent. Fatty Acids 62, 55-73 https://doi.org/10.1054/plef.1999.0124
  5. Aouadi, R, A Aouidet, A Elkadhi, C. B. Rayana, H Jaafoura, B. Tritar, and K Nagati. 2000. Effects of fresh garlic (Allium sativum) on lipid metabolism in male rats. Nutr. Research 20, 273-280 https://doi.org/10.1016/S0271-5317(99)00159-1
  6. Augusti, K T. and C. G Sheela. 1996. Antiperoxide effect of S-allyl cysteine sulfoxide, an insulin secretagogue, in diabetic rats. Experientia 52, 115-120 https://doi.org/10.1007/BF01923354
  7. Bane*e, S. K, M. Maulik, S. C. Mancahanda, A K Dinda, S. K Gupta, and S. K Maulik. 2002. Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic. Life Sci. 70, 1509-1518 https://doi.org/10.1016/S0024-3205(01)01514-4
  8. Barabol, V. A and E. N. Shestakova. 2004. Selenium: the biological role and antioxidant activity. Ukr. Biokhim. Zh. 76, 23-32
  9. Borek, C. 2001. Antioxidant health effects of aged garlic extract. J. Nutr. 131, 1010-1015
  10. Choi, S. D., Y. Baek, and Y. S. Kwak. 2007. Effects of diaphragm breathing and garlic powder intake on body composition, heart rate, blood pressure and immunoglobin in middle-aged male smokers. J. Life Sci. 17, 1266-1271
  11. Criswell, D., S. Powers, S. Dodd, J. Lawler, W. Edwards, K Renshler, and S. Grinton. 1993. High intensity training-induced changes in skeletal muscle antioxidant enzyme activity. Med. Sci. Sports Exerc. 25, 1135-1140 https://doi.org/10.1016/0034-5687(96)00057-6
  12. Dhawan, V. and S. Jain. 2004. Effect of garlic supplementation on oxidized low density lipoproteins and lipid peroxidation in patients of essential hypertension. Mol. Cell Biochem. 266, 109-115 https://doi.org/10.1023/B:MCBI.0000049146.89059.53
  13. Dillon, S. A, G M. Lowe, D. Billington, and K Rahman. 2002. Dietary supplementation with aged garlic extract reduces plasma and urine concentrations of 8-iso-prostaglandin F(2 alpha) in smoking and nonsmoking men and women. J. Nutr. 132, 168-171
  14. Duda, G, J. Suliburska, and D. Pupek-Musialik. 2008. Effects of short-term garlic supplementation on lipid metabolism and antioxidant status in hypertensive adults. Pharmacol. Rep. 60, 163-170
  15. Durak I., M. Kavutcu, B. Aytac, E. Devrim, H Ozbek and H S. Ozturk. 2004. Effects of garlic extract consumption on blood lipid and oxidant/ antioxidant parameters in humans with high blood cholesterol. J. Nutr. Biochem. 15, 373-377 https://doi.org/10.1016/j.jnutbio.2004.01.005
  16. Friedewald, W. T., R I. Levy, and D. S. Fredricson. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without was of the preparative ultracentrifuge. Clin. Chem. 18, 499-506
  17. Gebhart, R 1995. Amplication of palmitate-induced inhibition of cholesterol biosynthesis in cultured rat hepatocytes by garlic-derives organosulfur compounds. Phytomedicine 2, 29-33 https://doi.org/10.1016/S0944-7113(11)80045-0
  18. Gorinstein, S., M. Leontowicz. H. Leontowicz, Z. Jastrzebski, J. Drzewiecki, J. Namiesnik, Z. Zachwieja, H. Barton, Z. Tashma, E. Katrich, and S. Trakhtenberg. 2006a. Dose-dependent influence of commercial garlic (Allium sativum) on rats fed cholesterol-containing diet. J. Agric. Food Chem. 54, 4022-4027 https://doi.org/10.1021/jf058182+
  19. Gorinstein, S., M. Leontowicz. H Leontowicz, J. Drzewiecki, K Najman, E. Katrich, D. Barasch, K Yamamoto, S. Trakhtenberg. 2006b. Raw and boiled garlic enhances plasma antioxidant activity and improves plasma lipid metabolism in cholesterol-fed rats. Life Sci. 78, 655-663 https://doi.org/10.1016/j.lfs.2005.05.069
  20. Harauma, A and T. Moriguchi. 2006. Aged garlic extract improves blood pressure in spontaneously hypertensive rats more safely than raw garlic. J. Nutr. 136, 769-7737
  21. Ide, N. and B. H Lau. 2001. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-kappa b activation. J. Nutr. 131, 1020-1026
  22. Imai, L N. Ide, S. Nagae, T. Moriguchi, H Matsuura, and Y. Itakura. 1994. Antioxidants and radical scavenging effects of aged garlic extract and its constituents. Plant. Med. 60, 417-420 https://doi.org/10.1055/s-2006-959522
  23. Islam, M. S. and H. Choi. 2008. Comparative effects of dietary ginger (Zingiber officinale) and garlic (Allium sativum) investigated in a type 2 diabetes model of rats. J. Med. Food 11, 152-159 https://doi.org/10.1089/jmf.2007.634
  24. Jackson, M. L R H T. Edwards, and M. C. R Synons. 1985. Electron spin resonance studies of intact mammalian skeletal muscle. Biochim. Biophys. Acta. 847, 185-190 https://doi.org/10.1016/0167-4889(85)90019-9
  25. Jain, R C. and C. R Vyas. 1975. Garlic in alloxan-induced diabetic rabbits. Am. J. Clin. Nutr. 28, 684-685 https://doi.org/10.4103/0019-5359.13905
  26. Kang, J.-S., S.-H, Kim, P.-G, Kim, D.-W., Lee, and S. Ryu. 2009. Differences of wood vinegar ingestion and exercise training on blood lipids, MDA, and SOD activities in rats. J. Life Sci. 19, 1190-1199 https://doi.org/10.5352/JLS.2009.19.9.1190
  27. Kiesewetter, H, F. Jung, G Pindur, E. M. Jung, C. Mrowietz, and E. Wenzel. 1991. Effect of garlic on thrombocyte aggregation, microcirculation, and other risk factors. Int. J. Clin. Pharmacol. Ther. Toxicol. 29, 151-155
  28. Kumar, GRand K P. Reddy. 1999. Reduced nociceptive responses in mice with alloxan induced hyperglycemia after garlic (Allium sativum Linn.) treatment. Indian J. Exp. BioI. 37, 662-666
  29. Liu, 1. and Y. Y. Yeh. 2001. Water-soluble organosulfur compounds of garlic inhibit fatty acid and triglyceride syntheses in cultured rat hepatocytes. Lipids 36, 395-400 https://doi.org/10.1007/s11745-001-0734-4
  30. Lowry, O. H., N. J. Rosenbrough, A l. Farr, and R J. Randall. 1951. Protein measurement with the folin phenol reagent. J. BioI. Chem. 193, 265-275
  31. Marklund, S. 1., P. M. Andersen, l. Forsgren, P. Nilsson, P. I. Ohlsson, G Wikander, and A Oberg. 1997. Normal binding and reactivity of copper in mutant superoxide dismutase isol Wikafrom amyotrophic lateral sclerosis patients. J. Neurochem. 69, 675-68 https://doi.org/10.1046/j.1471-4159.1997.69020675.x
  32. Marques, E., J. Carvalho, J. M. Soares, F. Marques, and J. Mota. 2009. Effects of resistance and multicomponent exercise on lipid profiles of older women. Maturitas 63, 84-88 https://doi.org/10.1016/j.maturitas.2009.03.003
  33. Morihara, N., M. Dshijima, N. Kashimoto, I. Sumioka, T. Nishihama, M. Hayama, and H Takeda. 2006. Aged garlic extract ameliorates physical fatigue. BioI. Pharm. Bull. 29, 962-966 https://doi.org/10.1248/bpb.29.962
  34. Nakagawa, S., K Masamoto, H. Sumiyoshi, K Kunihiro, and T. Fuwa. 1980. Effect of raw and extracted-aged garlic juice on growth of young rats and their organs after peroral administration. J. Toxicol. Sci. 5, 91-112 https://doi.org/10.2131/jts.5.91
  35. Ohaeri, O. C. 2001. Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Biosci. Rep. 21, 19-24 https://doi.org/10.1023/A:1010425932561
  36. Pathak, U. N. and D. L. Gurubacharya. 2003. Non-pharmacological therapy of hyperlipidemia. Nepal Med. Coll. J. 5, 109-112. (Abstract)
  37. Rahman, K 2001. Historical perspective on garlic and cardiovascular disease. J. Nutr. 131, 977-9790
  38. Saitoh, K 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 90, 37-43 https://doi.org/10.1016/0009-8981(78)90081-5
  39. Sen, C. K 1995. Oxidants and antioxidants in exercise. J. Appl. Physiol. 79, 675-686
  40. Shin, S. H. and M. K Kim. 2004. Effect of dried powders or ethanol extracts of garlic flesh and peel on lipid metabolism and antithrombogenic capacity in 16-month-old rats. Kor. J. Nutr. 37, 515-524
  41. Silagy, C. and A Neil. 1994. Garlic as a lipid lowering agent--a meta-analysis. J. R. Call. Physicians Lond. 28, 39-45
  42. Slowing, K, P. Ganado, M. Sanz, E. Ruiz, and T. Tejerina. 2001. Study of garlic extracts and fractions on cholesterol plasma levels and vascular reactivity in cholesterol-fed rats. J. Nutr. 131, 994-999
  43. Thomson, M., K K AI-Qattan, T. Bordia, and M. Ali. 2006. Including garlic in the diet may help lower blood glucose, cholesterot and triglycerides. J. Nutr. 136, 800-802
  44. Vinson, J. A, Y. Hao, X. SUI and 1. Zubik. 1998. Phenol antioxidant quantity and quality in foods: vegetables. J. Agri. Food Chem. 46, 3630-3634 https://doi.org/10.1021/jf980295o
  45. Warshafsky, S., R. S. Kamer, and S. l. Sivak. 1993. Effect of garlic on total serum cholesterol. A meta-analysis. Ann. Intern. Med. 119, 599-605 https://doi.org/10.1059/0003
  46. Yasuda, A, M. Natsume, K Sasaki, S. Baba, Y. Nakamura, M. Kanegae, and S. Nagaoka. 2008. Cacao procyanidins reduce plasma cholesterol and increase fecal steroid excretion in rats fed a high-cholesterol diet. Biofactors 33, 211-223 https://doi.org/10.1002/biof.5520330307
  47. Yeh, Y. Y. and l. Liu. 2001. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. J. Nutr. 131, 989-993
  48. Yoon, G. A 2006. Effect of garlic supplement and exercise on plasma lipid and antioxidant enzyme system in rats. Kor. J. Nutr. 39, 3-10

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