Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
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DNA Separation Chips Using Asymmetrically-Switched Nonuniform Electric Fields

비대칭 교차전기장의 불균일 분포를 이용한 DNA 분리 소자

  • 이소연 (한국과학기술원 바이오및뇌공학과, 디지털나노구동연구단) ;
  • 조영호 (한국과학기술원 바이오및뇌공학과 및 기계공학과, 디지털나노구동연구단)
  • Published : 2009.03.01
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Abstract

We present the experimental study to realize a DNA separation chip using asymmetrically-switched nonuniform electric fields. The DNA separation chip redistributes DNA molecules within a specific area based on the size- and field-dependent nonlinearity of DNA drift velocity. The present chip is composed of a width variable channel to distribute nonuniform electric field, a DNA loading slit and a pair of electrodes to apply electric field. We focus on the design of DNA separation chips with identifying the nonlinearity of DNA drift velocity using three different DNA molecules (11.1kbp, 15.6kbp, and 48.5kbp) in the chips. It is demonstrated that different size of DNA shows different net migration in different direction under the asymmetrically-switched nonuniform electric field.

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References

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