Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anticancer Activity on Ethanolic Extract of the Masou Salmon (Oncorhynchus masou) in vitro and in vivo

산천어(Oncorhynchus masou) 에탄올 추출물의 in vitro 및 in vivo에서 항암활성

  • Oh, Hyun-Taek (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • Ham, Seung-Shi (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
  • 오현택 (강원대학교 BT특성화학부(대학) 식품생명공학) ;
  • 정미자 (강원대학교 BK21 사업단(뉴트라슈티컬 바이오)) ;
  • 함승시 (강원대학교 BT특성화학부(대학) 식품생명공학)
  • Published : 2009.02.28
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Abstract

The cytotoxic activity against human cancer cells and anti-tumor effect in Balb/c mice of a 70% ethanol extract from masou salmon (MSE) was investigated. The cancer cell lines including human breast adenocarcinoma (MCF-7), human lung carcinoma (A549), human hepatoblastoma (HepG2), human gastric carcinoma (AGS), human cervical adenocarcinoma (HeLa) and transformed primary human embryonal kidney (293) exposed to MSE decreased cell viability as indicated by the MTT assay. The MSE shows significant cytotoxicity on MCF-7, A549, HepG2, AGS and HeLa cells, and are more active than 293 cells. The treatment with 1 mg/mL MSE resulted in 9.2%, 12.7%, 16.6%, and 16.9% cell survival against A549, MCF-7, HepG2, and AGS cells, respectively. Moreover, anticancer effect in vivo of MSE was tested in the animal system using Balb/c mice transplanted sarcoma-180 cells. MSE showed inhibition of tumor growth and the rate of inhibition was 44.7% and 55.7% at the 25 mg/kg body weight and 250 mg/kg body weight, respectively. Thus, we suggest that MSE could be a beneficial material for human cancer prevention.

산천어 70% 에탄올 추출물(MSE)이 암세포 성장억제와 Balb/c 마우스에서의 종양 성장 억제에 미치는 영향을 알아보았다. 인간 자궁암(HeLa), 간암(HepG2), 유방암(MCF-7), 위암(AGS), 폐암(A549) 세포 그리고 인간신장 정상세포 (293)에 MSE을 처리했을 때 세포생존율이 감소하였고 이들 감소율을 MTT assay로 알아보았다. MSE는 정상세포 293에서보다 인간 암세포주인 MCF-7, A549, HepG2, AGS 그리고 HeLa세포에서 현저하게 더 높은 세포독성이 나타났다. 시료 최고농도인 1 mg/mL를 인간 폐암세포(A549), 유방암세포(MCF-7), 간암세포(HepG2) 및 위암세포(AGS)에 처리한 결과 각각 9.2%, 12.7%, 14.6% 및 16.9%의 세포 생존율을 나타내었다. 더하여 복수암 세포(sarcoma-180)를 이용한 실험동물(Balb/c mice)에서의 고형암 성장 억제 효과를 알아보았으며, 각각 25 mg/kg body weight와 250 mg/kg body weight의 MSE를 투여하였을 때 종양 성장 억제율은 각각 44.7%와 55.7%였다. 따라서 우리는 MSE가 암 예방을 위해 인간에게 유익한 기능성 소재일 것이라는 것을 제안하였다.

Keywords

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