Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Hemopoietic Effect of Extracts from Four Parts of Deer Antler on Phenylhydrazine-Induced Hemolytic Anemia in Female Rats

Phenylhydrazine으로 유도한 용혈성 빈혈 흰쥐에 대한 녹용 추출물의 조혈 효과

  • Lee, Mi-Ra (Dept. of Food Science and Technology, Chungnam National University) ;
  • Kim, Hyun-Ho (Dept. of Food Science and Technology, Chungnam National University) ;
  • Jo, Hyun-Ho (Dept. of Food Science and Technology, Chungnam National University) ;
  • Kang, Hyo-Jin (Dept. of Food Science and Technology, Chungnam National University) ;
  • Gu, LiJuan (Dept. of Food Science and Technology, Chungnam National University) ;
  • Ly, Sun-Young (Dept. of Food and Nutrition, Chungnam National University) ;
  • Lee, Chung-Ha (DBIO Research Institute) ;
  • Kim, Seung-Mi (DBIO Research Institute) ;
  • Yang, Sun-Ah (DBIO Research Institute) ;
  • Mo, Eun-Kyung (DBIO Research Institute) ;
  • Sung, Chang-Keun (Dept. of Food Science and Technology, Chungnam National University)
  • Published : 2009.12.31
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Abstract

This study was to investigate the protection of the extracts from four parts of deer antler in an anemia model induced by intravenous injection of phenylhydrazine·HCl (PHZ) at 10 mg/kg for 4 days. After PHZ injection, female Sprague-Dawley rats were administrated partial deer antler extract (200 mg/kg/day, p.o.) daily for 1 week. Results showed that sever hemolysis was induced by PHZ. For antler extract-treated groups, the concentration of hemoglobin, hematocrit and red blood cells number increased much more significantly than PHZ-treated group. Upper antler extract-treated group was more remarkable than other parts in suppressing the increase of reticulocyte in whole blood. Moreover, antler extract administration significantly improved serum erythropoietin concentration. The activity of $\delta$-aminolevulinic acid dehydrates (ALAD) in liver homogenate was increased in antler extract-treated groups, especially middle and base extract-treated groups showed statistical significance. These results could be concluded that the deer antler extract improved anemia induced by PHZ injection through improving hematological values, serum EPO value, ALAD enzyme activity.

본 연구는 부위별 녹용 추출물이 PHZ 투여로 용혈성 빈혈을 유도한 흰쥐의 조혈작용에 대한 효과를 검증하고자 하였다. 4주령의 암컷 Sprague-Dawley 흰쥐에 PHZ 10 mg/kg을 4일간 미정맥 주사하여 용혈성 빈혈을 유발한 후 각 부위별 녹용 추출물 200 mg/kg을 일주일간 경구투여 하였다. 용혈성 빈혈 유발군인 PHZ군은 hemoglobin, hematocrit치, 적혈구수가 대조군에 비해 유의적으로 감소하였고, 망상적 혈구수는 유의적으로 증가하였다. 반면, 녹용 추출물 군에서 는 hemoglobin, hematocrit치, 적혈구수가 PHZ 투여군보다 증가하였다. 특히 분골, 상대, 중대 추출물 투여 시 유의성있게 증가하였다. 상대 추출물 투여군에서는 망상적혈구수 증가가 유의적으로 억제되었다. PHZ 투여로 증가된 혈청 EPO 함량은 녹용 추출물 투여군에서 모두 정상적으로 회복 되었다. Hemoglobin 합성에 관여하는 $\delta$-ALAD 활성은 녹용 추출물 투여군에서 활성이 증가되는 결과를 보여주었다. 따라서 녹용 추출물은 PHZ으로 유도한 용혈성 빈혈에 대한 바람직한 조혈효과가 있는 기능성 물질로 사료된다.

Keywords

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