한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제38권12호
  • /
  • Pages.1712-1717
  • /
  • 2009
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

흰쥐 모델에서 Capsaicin이 소장 콜레스테롤 및 중성지방 흡수율에 미치는 영향

Effect of Enteral Capsaicin on the Lymphatic Absorption of Cholesterol and Fats in Rats

  • 서윤정 (국립창원대학교 식품영양학과) ;
  • 김주연 (국립창원대학교 식품영양학과) ;
  • 노상규 (국립창원대학교 식품영양학과)
  • Seo, Yun-Jung (Dept. of Food and Nutrition, Changwon National University) ;
  • Kim, Ju-yeoun (Dept. of Food and Nutrition, Changwon National University) ;
  • Noh, Sang-K. (Dept. of Food and Nutrition, Changwon National University)
  • 발행 : 2009.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 capsaicin 투여가 콜레스테롤을 포함한 지방의 소장 흡수율에 어떠한 영향을 미치는지를 조사하기 위해서 설계되었다. 지방의 소장 흡수율을 측정하기 위해서 흰쥐 소장 지방 흡수율 측정모델을 이용하여, 쥐의 십이지장으로 capsaicin이 포함된 지질유화액을 8시간 동안 지속적으로 주 입하면서 8시간 동안 림프관으로 분비되는 14C-cholesterol, α-tocopherol, 지방산을 분석하여 capsaicin에 의한 영향을 비교하였다. 8시간 동안 분비된 림프액의 양은 대조군과 capsaicin군 간에 유의적인 차이를 나타내지 않았다. α-tocopherol의 흡수율에서도 유의적인 차이가 없었다. 그러나 총 8시간 그리고 시간대별 14C-cholesterol 흡수율은 대조군과 비교하였을 때 capsaicin군에서 유의적인 차이로 감소되는 경향을 보였다. 또한 중성지방의 흡수율의 비교에서도 팔미트산, 스테아르산, 아라키돈산, DHA의 흡수율은 두 군 간에 유의적인 차이가 없었으나 지방유화액을 통해 주입한 올레인산의 흡수율에서는 유의적인 감소현상을 보였다. 결론적으로, capsaicin의 응용성은 비만 치료 및 지방대사 조절과 관련해 특히, 소장 지방 흡수대사에 치중한 식이적인 수단의 근거로써 활용이 기대된다.

Capsaicin has been shown to affect lipid metabolism. However, it is currently not known whether capsaicin would lower the intestinal absorption of cholesterol. Thus, this study was conducted to investigate the effect of intraduodenally infused capsaicin on the lymphatic absorption of cholesterol and lipids in rats. Male Sprague-Dawley rats weighing 300-350 g were starved for 16 hr and the mesenteric lymph duct was cannulated. Each rat was infused at 3.0 mL/hr for 8 hr via the duodenal catheter with a lipid emulsion, which contained 33.3 kBq [$^{14}C$]-cholesterol, 20.7 μmol cholesterol, 452 μmol triolein, 3.1 μmol $\alpha$-tocopherol, and 396.0 μmol Na-taurocholate without (control) or with 5.0 mg capsaicin in 24 mL PBS buffer (pH 6.4). Simultaneously, lymph was collected hourly for 8 hr. There was no significant difference in lymph flow between the groups. However, the lymphatic absorption of 14C-cholesterol for 8 hr was significantly lower in rats infused with capsaicin than in those infused with no capsaicin. Also, the output of oleic acid for 8 hr was significantly decreased by capsaicin. However, the intestinal absorption of $\alpha$-tocopherol did not differ between the groups. The results indicate that the luminal infusion of capsaicin inhibits the intestinal absorption of cholesterol and lipids in rats.

키워드

참고문헌

  1. Tyrovolas S, Panaqiotakos DB. 2009. The role Mediterranean type of diet on the development of cancer and cardivascular diesease, in the elderly: A systematic review. Maturitas 163: 1461-1462
  2. Ebrahim S, Sung JH, Song YM, Ferrer RL, Debbie AL, Smith GD. 2006. Serum cholesterol, haemorrhagic stroke, ischaemic stroke, and myocardial infarction: Korean national health system prospective cohort study. BMJ 333: 22-27 https://doi.org/10.1136/bmj.38855.610324.80
  3. Vahouny GV, Chalcarz W, Satchithanandam S, Adamson I, Klurfeld DM, Kritchevsky D. 1984. Effect of soy protein and casein intake on intestinal absorption and lymphatic transport of cholesterol and oleic acid. Am J Clin Nutr 40: 1156-1164 https://doi.org/10.1093/ajcn/40.6.1156
  4. Loest HB, Noh SK, Koo SI. 2002. Green tea extract inhibits the lymphatic absorption of cholesterol and alpha-tocopherol in ovariectomized rats. J Nutr 132: 1282-1288 https://doi.org/10.1093/jn/132.6.1282
  5. Koo SI, Noh SK. 2007. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutr Biochem 18: 179-183 https://doi.org/10.1016/j.jnutbio.2006.12.005
  6. Tatsuo W, Teruo K, Kazuo I. 1986. Enhancement by capsaicin of energy metabolism in rats through secretion of catecholamine. Agric Biol Chem 51: 75-79
  7. Henderson DE, Slickman AM, Henderson SK. 1999. Quantitative HPLC determination of the antioxidant activity of capsaicin on the formation of lipid hydroperoxides of linoleic acid: a comparative study against BHT and melatonin. J Agric Food Chem 47: 2563-2570 https://doi.org/10.1021/jf980949t
  8. Kempaiah RK, Manjunatha H, Srinivasan K. 2005. Protective effect of dietary capsaicin on induced oxidation of low- density lipoprotein in rats. Mol Cell Biochem 275: 7-13 https://doi.org/10.1007/s11010-005-7643-3
  9. Surh YJ, Lee RC, Park KK, Mayne ST, Liem A, Miller JA. 1995. Chemopreventive effects of capsaicin and diallyl sulfide against mutagenesis or tumorigenesis by vinyl carbamate and N-nitrosodimethylamine. Carcinogenesis 16: 2467-2471 https://doi.org/10.1093/carcin/16.10.2467
  10. Reddy A, Lokesh BR. 1994. Studies on the antiinflammatory activity of spice principles and dietary n-3 fatty acids on carrageenan-induced inflammation in rat. Ann Nutr Metab 38: 349-358 https://doi.org/10.1159/000177833
  11. Green BG, Hayes JE. 2004. Reduced heat sensitivity and epidermal nerve fiber immunostaining following single applications of a high-concentration capsaicin patch. Pain 111: 360-367 https://doi.org/10.1016/j.pain.2004.07.017
  12. Buck SH, Burks TF. 1986. The neuropharmacology of capsaicin: review of some recent observations. Pharmacol Rev 38: 179-226
  13. Hsu CL, Yen GC. 2007. Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells. J Agric Food Chem 55: 1730-1736 https://doi.org/10.1021/jf062912b
  14. Watanabe T, Kawade T, Yamamoto M, Iwai K. 1987. Capsaicin, a pungent principle of hot red pepper, evokes catecholamine secretion from the adrenal medulla of anesthetized rats. Biochem Biophys Res Commun 142: 259-564 https://doi.org/10.1016/0006-291X(87)90479-7
  15. Kawada T, Hagihara KI, Iwai K. 1986. Effects of capsaicin on lipid metabolism in rats fed a high fat diet. J Nutr 116: 1272-1278 https://doi.org/10.1093/jn/116.7.1272
  16. Srinivasan MR, Satyanarayana MN. 1989. Effect of capsaicin on skeletal muscle lipoprotein lipase in rats fed high fat diet. Indian J Exp Biol 27: 910-912
  17. Tso P, Balint JA. 1986. Formation and transport of chylomicrons by enterocytes to the lymphatics. Am J Physiol 250: G715-G726
  18. Reeves PG, Nielsen FH, Fahey GC Jr. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  19. Koo SI, Noh SK. 2001. Phosphatidylcholine inhibits and lysophosphatidylcholine enhances the lymphatic absorption of $\alpha$-tocopherol in adult rats. J Nutr 131: 717-722 https://doi.org/10.1093/jn/131.3.717
  20. Folch PJ, Lees M, Sloane-Stanley GM. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509
  21. Zaspel BJ, Csallany AS. 1983. Determination of alpha-tocopherol in tissues and plasma by high-performance liquid chromatography. Anal Biochem 130: 146-150 https://doi.org/10.1016/0003-2697(83)90661-9
  22. Liu JF, Huang CJ. 1995. Tissue $\alpha$-tocopherol retention in male rats is compromised by feeding diets containing oxidized frying oil. J Nutr 125: 3071-3080
  23. Slover HT, Lanza E. 1979. Quantitative analysis of food fatty acids by capillary gas chromatography. J Am Oil Chem Soc 56: 933-943 https://doi.org/10.1007/BF02674138
  24. Kwon MJ, Song YS, Choi MS, Song YO. 2003. Red pepper attenuates cholesteryl ester transfer protein activity and atherosclerosis in cholesterol-fed rabbits. Clin Chim Acta 332: 37-44 https://doi.org/10.1016/S0009-8981(03)00118-9
  25. Noh SK, Koo SI. 2001. Intraduodenal infusion of lysophosphatidylcholine restores the intestinal absorption of vitamins A and E in zinc-deficient rats. Exp Biol Med 226: 342-348 https://doi.org/10.1177/153537020122600412
  26. Pownall HJ, Hickson DL, Smith LC. 1983. Transport of biological lipophiles: Effect of lipophile structure. J Am Chem Soc 105: 2440-2445 https://doi.org/10.1021/ja00346a055
  27. Shu W, Noh SK, Koo SI. 2006. Green tea catechins inhibits pancreatic phospholipase A2 and intestinal absorption of lipids in ovariectomized rats. J Nutr Biochem 17: 492-498 https://doi.org/10.1016/j.jnutbio.2006.03.004
  28. Saito A, Nakamura K, Hori Y, Yamamoto M. 1999. Effects of capsaicin on serum triglycerides and free fatty acid in olive oil treated rats. Int J Vitam Nutr Res 69: 337-340 https://doi.org/10.1024/0300-9831.69.5.337

피인용 문헌

  1. Cytotoxicity of Extracts from Korean Pepper (Capsicum annuum L.) by Extraction Solvents and Plant Parts vol.22, pp.5, 2014, https://doi.org/10.7783/KJMCS.2014.22.5.369
  2. Quality Characteristics of Cookies Added with Capsaicin Powder vol.27, pp.5, 2014, https://doi.org/10.9799/ksfan.2014.27.5.921
  3. Antioxidant Activity of Capsaicinoid in Canola Oil vol.60, pp.24, 2009, https://doi.org/10.1021/jf301744q
  4. Capsaicinoids lower plasma cholesterol and improve endothelial function in hamsters vol.52, pp.1, 2013, https://doi.org/10.1007/s00394-012-0344-2
  5. Capsaicinoids but Not Their Analogue Capsinoids Lower Plasma Cholesterol and Possess Beneficial Vascular Activity vol.62, pp.33, 2009, https://doi.org/10.1021/jf502888h