Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Different oxidative burst patterns occur during host and nonhost resistance responses triggered by Xanthomonas campestris in pepper

  • Kwak, Youn-Sig (Department of Applied Biology & Enviromental Sciences, Research Institute of Life Science, Gyeongsang National University) ;
  • Han, Ki-Soo (Department of Applied Biology & Enviromental Sciences, Research Institute of Life Science, Gyeongsang National University) ;
  • Lee, Jung-Han (Department of Applied Biology & Enviromental Sciences, Research Institute of Life Science, Gyeongsang National University) ;
  • Lee, Kyung-Hee (The Aging-associated Disease Research Center and Department of Microbiology, Yeungnam University College of Medicine) ;
  • Chung, Woo-Sik (Enviromental Biotechnology National Core Research Center and Division of Applied Life Science(BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Mysore, Kirankumar S. (Plant Biology Division, Samuel Roberts Noble Foundation) ;
  • Kwon, Young-Sang (Department of Applied Biology & Enviromental Sciences, Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Hee-Kyu (Department of Applied Biology & Enviromental Sciences, Research Institute of Life Science, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Instrument Facility, Gyeongsang National University)
  • Published : 2009.09.30
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Abstract

The hypersensitive reaction (HR) is the most common plant defense reaction against pathogens. HR is produced during both host- and nonhost-incompatible interactions. Several reports suggest that similarities exist between host and nonhost resistances. We assayed the pattern of generation of reactive oxygen species (ROS) and scavenging enzyme activities during nonhost pathogen-plant interactions (Xanthomonas campestris pv. campestris/Capsicum annuum L.) and incompatible host pathogen-plant interactions (Xanthomonas campestris pv. vesicatoria race1/Capsicum annuum L.). Both ${O_2}^-\;and\;H_2O_2 $ accumulated much faster during nonhost resistance when compared to the host resistance. The scavenging enzyme activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) were also different during the host- and nonhost-incompatible interactions. CAT activity was much higher during nonhost resistance, and several new isozymes of SOD and POX were detected during nonhost resistance when compared to the host resistance. Lipoxygenase (LOX) activity was higher in host resistance than nonhost resistance during the early stages of infection. Interestingly, the nitric oxide (NO) radical accumulated equal amounts during both host and nonhost resistance at early stages of infection. Further studies are needed to determine the specific pathways underlying these differences between host and nonhost resistance responses.

Keywords

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