Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Transfer of SOD2 or NDP kinase 2 genes into purebred lines of petunia

  • Lee, Su-Young (Floricultural Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Han, Bong-Hee (Floricultural Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Noh, Eun-Woon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2009.06.30
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Abstract

The transfer of Mn-Superoxide Dismutase (SOD2) gene, complex gene (SA) of CuZnSOD and ascorbate peroxidase (APX), and NDP kinase 2 (NDPK2) gene into Korean 4 cultivars (cvs. Millenium White, Glory Blue, Glory Red, and Glory Purple) and 15 purebred lines of petunia was conducted using Agrobaterium-mediated technique. Two (Wongyo A2-16 and A2-36) of 15 purebred lines and one (cv. Glory Red) of 4 cultivars were effective for the transfer of SOD2 gene. The putative transgenic plants survived on the 2nd selection medium were 124. From PCR analysis, 118 (derived from 4 cultivars and 2 purebred lines) of 124 plants were confirmed to contain marker (npt II ) gene, while 58 of 118 plants did not have target genes. There were no plants with both npt II and SA genes. Twenty seven of 28 SOD2 transgenic plants were re-confirmed as transformants by Sothern analysis. SOD2 and NDPK2 genes were expressed in the transgenic petunias as the ratio of 77.8 to 100.0 % and 23.5%, respectively. T1 seeds were obtained from 36 acclimated transgenic plants (SOD2 34 plus NDPK2) in a glasshouse by self-pollination.

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References

  1. Chandler SF and Lu CY (2005) Biotechnology in ornamental horticulture. In Vitro Cell. Biol. Plant 41:591-601 https://doi.org/10.1079/IVP2005681
  2. Fang GC, Hanau RM, Vaillancourt LJ (2002) The SOD2 gene, encoding a manganese-type superoxide dismutase, is upregulated during conidiogenesis in the plant pathogenic fungus Colletotrichum graminicola. Fungal Genetics, and Biology 36:155-165 https://doi.org/10.1016/S1087-1845(02)00008-7
  3. Haejeong M, Lee B, Choi G, Shin D, Prasad DT, Lee O, Kwak SS, Kim DH, Nam J, Bahk J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MARKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. PNAS. 100:358-363 https://doi.org/10.1073/pnas.252641899
  4. Kim KY, Kwon SY, Lee HS, Hur Y, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol. Biol. 51:831-838 https://doi.org/10.1023/A:1023045218815
  5. Kim JS, Lee BH, Kwon SY, Kim YH, Kim SH, Cho KY (2005) Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts to several herbicides. Kor. J. Plant Biotech. 32:97-103 https://doi.org/10.5010/JPB.2005.32.2.097
  6. Lee SY, Han BH (2008) Development of petunia resistant to environmental stress. In: National Horticultural Research Institute (eds) Report of horticultural experimental studies in 2007 pp: 333-342 (in Korean)
  7. Lee SY, Woo JG, Han BH, Bang CS, Oh DG, Huh KY (2005) Superoxide dismutase(SOD) gene transferred into cultivars and breeding lines of Petunia hybrid. In: The Korean Society of Plant Biotechnology (eds). Platform technology for plant bioproduct. p 217
  8. Lee SH, Ahsan N, Lee KW, Lim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH (2007) Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J. Plant Physiology 164:1626-1638 https://doi.org/10.1016/j.jplph.2007.01.003
  9. National Horticultural Research Institute (2006) New cultivars of ornamental crops. pp 47-51
  10. Shibata M (2008) Importance of genetic transformation in ornamental plant breeding. Plant Biotech. 25:3-8 https://doi.org/10.5511/plantbiotechnology.25.3
  11. Tang L, Kwon SY, Kim MD, Kim JS, Kwak SS, Lee HS (2007) Enhanced tolerance to oxidative stress of transgenic potato (cv. Superior) plants expressing SOD and APX in chloroplasts. Kor. J. Plant Biotech. 34:299-305 https://doi.org/10.5010/JPB.2007.34.4.299
  12. Tang L, Kwon SY, Yun DJ, Kwak SS, Lee HS (2004a) Selection of transgenic potato plants expressing NDP Kinase 2 gene with enhanced tolerance to oxidative stress. Kor. J. Plant Biotech. 31:19-195 https://doi.org/10.5010/JPB.2004.31.3.191
  13. Tang L, Kwon SY, Kwak SS, Sung CK, Lee HS (2004b) Selection of transgenic potato plants expressing NDP Kinase 2 gene with enhanced tolerance to oxidative stress. Kor. J. Plant Biotech. 31:109-113 https://doi.org/10.5010/JPB.2004.31.3.191

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