Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Molecular biological characteristics and analysis using the specific markers of leaf folder-resistant GM rice

혹명나방저항성 GM 벼의 분자생물학적 특성 및 특이 마커를 이용한 검정

  • 신공식 (농촌진흥청 국립농업과학원) ;
  • 이시명 (농촌진흥청 국립농업과학원) ;
  • 임선형 (농촌진흥청 국립농업과학원) ;
  • 우희종 (농촌진흥청 국립농업과학원) ;
  • 조현석 (농촌진흥청 국립농업과학원) ;
  • 이경렬 (농촌진흥청 국립농업과학원) ;
  • 이명철 (농촌진흥청 첨단농업과) ;
  • 권순종 (농촌진흥청 국립농업과학원) ;
  • 서석철 (농촌진흥청 국립농업과학원)
  • Published : 2009.06.30
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Abstract

In recent years, several genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by various agricultural biotechnological companies. Commercialization of GM crops will be required the assesment of risks associated with the release of GM crops. In advance of the commercial release of GM crops, developer should submit the several information on GM crops for approval. In this study, we carried out to provide the molecular data for the risk assessment of GM rice containing insect-resistant gene, modified Cry1Ac (CryIAc1). Through the molecular analysis with CryIAc1 induced GM rice, we confirmed the steady integration and expression of transgene, the transgene copy number, the adjacent region sequences of inserted gene into rice genome, and the transgene stability in progenies. For the qualitative PCR detection methods, specific primer pairs were designed on the basis of integration sequences, and construct- and event-specific detection markers were developed for leaf folder-resistant rice, Cr7-1 line. From these results, we demonstrated that the molecular data and the PCR detection methods of leaf folderresistant GM rice could be acceptable to conduct the biosafety and environment risk assessment.

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