Journal of Pharmaceutical Investigation

  • 제39권6호
  • /
  • Pages.393-400
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  • 2009
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  • 2093-5552(pISSN)
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  • 2093-6214(eISSN)
한국약제학회 (The Korean Society of Pharmaceutical Sciences and Technology)
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광학 영상을 위한 Cy5.5가 결합된 생체적합성 고분자 마이셀 나노입자의 제조 및 특성분석

Preparation and Characterization of Cy5.5-conjugated Biocompatible Polymeric Micellar Nanoparticles for Optical Imaging

  • 김효정 (한국화학연구원 바이오소재센터,경희대학교 약학대학) ;
  • 김병진 (경희대학교 약학대학) ;
  • 이하영 (한국유나이티드제약) ;
  • 정석현 (한국화학연구원 바이오소재센터) ;
  • 정서영 (한국화학연구원 바이오소재센터) ;
  • 육순홍 (경희대학교 약학대학) ;
  • 신병철 (한국화학연구원 바이오소재센터) ;
  • 성하수 (한국화학연구원 바이오소재센터) ;
  • 최연웅 (한국화학연구원 바이오소재센터) ;
  • 하대철 (한국유나이티드제약) ;
  • 조선행 (한국유나이티드제약) ;
  • 이수민 (한남대학교 화학과)
  • Kim, Hyo-Jeong (Biomaterials Research Center, Korea Research Institute of Chemical Technology,Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Byung-Jin (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Lee, Ha-Yeong (Korea United Pharm) ;
  • Jung, Suk Hyun (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Jeong, Seo-Young (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Yuk, Soon-Hong (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Shin, Byung-Cheo (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Seong, Ha-Soo (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Youn-Woong (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Ha, Dae-Chul (Korea United Pharm) ;
  • Choi, Sun-Hang (Korea United Pharm) ;
  • Lee, Soo-Min (College of chemistry, Hannam University)
  • 발행 : 2009.12.20
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초록

PHEA (hydroxyethyl-aspartamide)-mPEG (methoy-polyethyleneglycol)-$C_{16}$ (hexadecylamine)-ED (ethylenediamine) was prepared as a drug delivery carrier. The structure and molecular weight of polymers were characterized by $^1H$-NMR and gel permeation chromatography. Micelle size and shape were measured by electro-photometer light scattering and transmission electron microscope. The mean diameter of micelles was 23 nm in aqueous solution. To evaluate the potential of these polymeric micelles as a drug carrier, PSI-mPEG-$C_{16}$-ED was conjugated with Cy5.5 for Near-Infrared Fluorescent (NIRF) based optical imaging. PSI-mPEG-$C_{16}$-ED-Cy5.5 was injected intravenously into mice (n=5) and in vivo NIRF imaging was performed during 48 h after injection. The biodistribution study at 24 h after injection showed the longcirculation property of PSI-mPEG-$C_{16}$-ED-Cy5.5. Therefore, PSI-mPEG-$C_{16}$-ED micelles could be a promising drug carrier and imaging agent.

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