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The Korean Society of Phycology (한국조류학회(藻類))
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Potential of Benthic Diatoms Achnanthes longipes, Amphora coffeaeformisand Navicula sp. (Bacillariophyceae) as Antioxidant Sources

  • Lee, Seung-Hong (Faculty of Applied Marine Science,Cheju National University) ;
  • Karawita, Rohan (Faculty of Applied Marine Science,Cheju National University) ;
  • Affan, Abu (Department of Oceanography,Cheju National University) ;
  • Lee, Joon-Baek (Department of Oceanography,Cheju National University) ;
  • Lee, Ki-Wan (Faculty of Applied Marine Science,Cheju National University) ;
  • Lee, Bae-Jin (Marine Bioprocess Co. Ltd.,Pukyong National University) ;
  • Kim, Dong-Woo (Central REcearch Center,Natural F&P Co.) ;
  • Jeon, You-Jin (Faculty of Applied Marine Science,Cheju National University)
  • Published : 2009.03.01
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Abstract

Recently, interest in plant-derived food additives has developed natural antioxidants, in order to alternate syn-thetic antioxidants with several disadvantages. In the present study, different organic fractions from solvent parti-tions of 80% methanol extract from Jeju benthic diatoms, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis was assessed for their potential antioxidant effects. Among the solvent fractions tested, n-hexane (80.4%) and 80% methanol extract (76.6%) from A. longipes, chloroform (63.2%) from Navicula sp. and n-hexane (67.4%) from A. cof-feaeformis were effective in DPPH free radical scavenging. Fractions of chloroform (53.4%) and n-hexane (53.1%) from A. longipes exhibited higher activities on $H_2O_2$ scavengin. Fraction of n-Hexane from A. longipes exhibited the highest hydroxyl radical scavenging activity and NO. scavenging activity (56.5% and 75.6%, respectively). Aqueous residue from A. coffeaeformis (75.6%) showed the highest metal chelating effect. chloroform and ethyl acetate frac-tion of all the diatoms exhibited significant antioxidant activities in lipid peroxidation inhibitory activity. In particu-lar, both chloroform and the ethyl ecetate fraction from A. longipes and A. coffeaeformis exhibited lipid peroxidation inhibitory activity significantly higher than that of $\alpha$- tocopherol. These data suggest that the Jeju benthic diatoms tested are rich in hydrophilic and hydrophobic antioxidative compounds with different antioxidative properties that can be applied in food industry.

Keywords

References

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