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마이크로세틀라이트 마커 분석을 이용한 남서태평양 일대에 서식하는 남방톱날꽃게(Scylla serrata)의 유전적 다양성

Genetic Diversity of the Mud Crab Scylla serrata in Micronesia based on Microsatellite Marker Analysis

  • 장요순 (한국해양연구원 해양생물자원연구부) ;
  • 이순길 (한국해양연구원 해양생물자원연구부) ;
  • 노충환 (한국해양연구원 동해연구소) ;
  • 오승용 (한국해양연구원 해양생물자원연구부)
  • 발행 : 2009.12.30

초록

Analysis of four microsatellite markers from Mud Crab Scylla serrata revealed that there is high level of genetic diversity within this species. Genetic diversity of S. serrata was calculated using allele diversity, observed heterozygosity, expected heterozygosity (Het-exp), polymorphic information content, gene differentiation and Nei's $D_{A}$ distance. Mean polymorphic information content value was 0.797, which reflected high level of polymorphism across the loci of S. serrata. The Palau population has the highest genetic diversity (Het-exp=0.871), while the Kosrae population has the lowest genetic diversity (Hetexp=0.806). However, the geographical genetic distance among S. serrata populations from Yab, Chuuk, Pohnpei, Kosrae, and Palau were low (0.2009${\sim}$0.3350). These results suggest that despite their wide distribution, S. serrata are no different in geographical genetic diversity within the five sampled locations.

키워드

참고문헌

  1. Blin N, Stafford DW (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 3(9):2303-2308 https://doi.org/10.1093/nar/3.9.2303
  2. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3):314-331
  3. Brown IW (1993) Mangrove crabs. In: Wright A, Hill L (eds), Nearshore marine resources of the South Pacific: Information for fisheries development and management, forum fisheries agency, Honiara, Solomon Islands, pp 609-642
  4. FAO (2008) Species fact sheet. Scylla serrata (null, 2001). FAO Species identification and data program. Text and table on the internet. http://www.fao.org/fishery/species/2637. Accessed 5 Mar 2009
  5. FRDC (2005) Methods for monitoring the abundance and habitat of the northern australian mud crab Scylla serrata. Fisheries Research & Development Council (FRDC) Project 2000/142 Final Repoprt pp 5-8
  6. Gopurenko D, Hughes JM (2002) Regional patterns of genetic structure among Australian populations of the mud crab, Scylla serrata (Crustacea : Decapoda): Evidence from mitochondrial DNA. Mar. Freshw. Res 53(5):849-857 https://doi.org/10.1071/MF01225
  7. Gopurenko D, Hughes JM, Jing M (2002) Identification of polymorphic microsatellite loci in the mud crab Scylla serrata (Brachyura: Portunidae). Mol Ecol Notes 2(4) 481-483 https://doi.org/10.1046/j.1471-8286.2002.00286.x
  8. Hänfling B, Weetman D (2003) Characterization of microsatellite loci for the Chinese mitten crab, Eriocheir sinensis. Mol Ecol Notes 3(1):15-17 https://doi.org/10.1046/j.1471-8286.2003.00336.x
  9. Hill BJ (1975) Abundance, breeding and growth of the crab Scylla serrata in two South African estuaries. Mar Biol 32(2):119-126 https://doi.org/10.1007/BF00388505
  10. Jensen PC, Bentzen P (2004) Isolation and inheritance of microsatellite loci in the Dungeness crab (Brachyura: Cancridae: Cancer magister). Genome 47(2):325-331 https://doi.org/10.1139/g03-135
  11. Keenan CP (1999) The fourth species of Scylla. In: Keenan CP, Blackshaw A (eds) Mud crab aquaculture and biology ACIAR proceedings, vol 78, pp 48-58
  12. Klinbunga S, Boonyapakdee A, Pratoomchat (2000) Genetic diversity and species-biagnostic markers of mud crab (Genus Scylla) in eastern Thailand determined by RAPD analysis. Mar Biotechnol 2(2):180-187
  13. Liu ZJ, Cordes JF (2004) DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238(1):1-37 https://doi.org/10.1016/j.aquaculture.2004.05.027
  14. Nei M (1972) Genetic distance between population. Am. Nat. 106(949):283-292 https://doi.org/10.1086/282771
  15. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583-590
  16. Nei M, Tajima F, Tateno Y (1983) Accuracy of estimated phylogenic trees from molecular data. J Mol Evol 19(2):153-170 https://doi.org/10.1007/BF02300753
  17. Olufowote JO, Xu Y, Chen X, Goto M, McCouch SR, Park WD, Beachell HM, Dilday RH (1997) Comparative evaluation of within-cultivar variation of rice (Oryza sativa L.) using microsatellite and RFLP markers. Genome 40(3):370-378 https://doi.org/10.1139/g97-050
  18. Ortí G, Pearse DE, Avise JC (1997) Phylogenetic assessment of length variation at a microsatellite locus. Proc Natl Acad Sci U S A 94(20):10745-10749 https://doi.org/10.1073/pnas.94.20.10745
  19. Ota T (1993) DISPAN. Pensylvania State University, PA. USA.
  20. Park S (2000) Microsatellite Toolkit for MS Excel 97 or 2000 (personel communication)
  21. Tepolt CK, Bagley MJ, Geller JB, Blum MJ (2006) Characterization of microsatellite loci in the European green crab (Carcinus maenas). Mol Ecol Notes 6(2):343-345 https://doi.org/10.1111/j.1471-8286.2006.01226.x
  22. Urbani N, Sevigny JM, Sainte-Marie B, Zadworny D, Kuhnlein U (1998) Identification of microsatellite markers in the snow crab Chionoecetes opilio. Mol Ecol 7(3):357-358 https://doi.org/10.1046/j.1365-294X.1998.00306.x
  23. Yi SK, Lee SK, Lee JM (2009). Preliminary study of seed production of the micronesian mud crab Scylla serrata(Crustacea: Portunidae) in Korea. Ocean and Polar Res 31(3):257-264 https://doi.org/10.4217/OPR.2009.31.3.257
  24. Zhongbao LI, Shaojing LI, Guizhong W (2004) Genetic diversity and differentiation of mud crab Scylla serrata populations from southeastern China. Acta Oceanol Sin 23(2):309-316

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