Advances in Traditional Medicine

  • 제9권4호
  • /
  • Pages.277-284
  • /
  • 2009
  • /
  • 2662-4052(pISSN)
  • /
  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
권호 표지
DOI QR코드

DOI QR Code

In vitro anti-oxidant activity of the leaves of Dillenia indica

  • Saha, Moni Rani (Department of Pharmacy, Stamford University) ;
  • Alam, Ashraful (Department of Pharmacy, Stamford University) ;
  • Hasan, Raquibul (Department of Pharmacy, Stamford University) ;
  • Akter, Raushanara (Department of Pharmacy, Stamford University) ;
  • Hossain, Mokarram (Department of Pharmacy, Stamford University) ;
  • Mazumder, Ehsanul Hoque (Jahangirnagar University, Saver, Dhaka, Laboratory of Pharmacognosy and Pharmacology, Department of Pharmacy, Stamford University) ;
  • Rana, Sohel (Jahangirnagar University, Saver, Dhaka, Laboratory of Pharmacognosy and Pharmacology, Department of Pharmacy, Stamford University)
  • 발행 : 2009.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The methanol extract of Dillenia indica was tested for antioxidant activity as determined by free radical scavenging of DPPH radical scavenging assay, reducing power, total antioxidant capacity measured by phosphomolybdenum method, total phenolic content and total flavonoids content determination assays. The extract showed significant activities in all antioxidant assays compared to the standard antioxidant in a dose dependent manner. In DPPH radical scavenging assay the $IC_{50}$ value of the extract was found to be 100.53${\mu}g/ml$ while ascorbic acid has the $IC_{50}$ value 58.92${\mu}g/ml$. Dillenia indica extract showed strong reducing power and total antioxidant capacity. Moreover, methanol extracts also possess high amount of phenolics and flavovonoids and expressed as gallic acid and rutin equivalent respectively. The remarkable activities exhibited in reactive oxygen species scavenging may attributed to the high amount of hydrophilic phenolics present in Dillenia indica.

키워드

참고문헌

  1. Abdille MH, Singh RP, Jayaprakasha GK, Jena BS. (2005) Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem. 90, 891-896 https://doi.org/10.1016/j.foodchem.2004.09.002
  2. Alam MA, Ghani A, Subhan N, Rahman MM, Haque MS, Majumder MM, Mazumder MEH, Akter RA, Nahar L, Sarker SD. (2008) Antioxidant and membrane stabilizing properties of the flowering tops of Anthocephalus cadamba. Nat. Prod. Commun. 3, 65-67
  3. Alam MA, Nyeem MAB, Awal MA, Mostofa M, Alam MS, Subhan N, Rahman MM. (2008) Antioxidant and hepatoprotective action of the crude methanolic extract of the flowering top of Rosa damascena. Orient. Pharm. Exp. Med. 8, 164-170 https://doi.org/10.3742/OPEM.2008.8.2.164
  4. Braca A, Sortino C, Politi M, Morelli I, Mendez J. (2002) Antioxidant activity of flavonoids from Licania licaniaeflora. J. Ethnopharmacol. 79, 379-381 https://doi.org/10.1016/S0378-8741(01)00413-5
  5. Branen AL. (1975) Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, J. Am. Oil Chem. Soc. 52, 59-63 https://doi.org/10.1007/BF02901825
  6. Deighton N, Brennan R, Finn C, Davies HV. (2000) Antioxidant properties of domesticated and wild Rubus species. J. Sci. Food Agric. 80, 1307-1313 https://doi.org/10.1002/1097-0010(200007)80:9<1307::AID-JSFA638>3.0.CO;2-P
  7. Diplock AT. (1997) Will the good fairies please prove us that vitamin E lessens human degenerative disease? Free Radic. Res. 27, 511-532 https://doi.org/10.3109/10715769709065791
  8. Duh PD, Tu YY, Yen GC. (1999) Antioxidant activity of water extract of Harng Jyur (Chrysanthemum moifolium Ramat). Lebensm–Wiss Technol 32, 269-277 https://doi.org/10.1006/fstl.1999.0548
  9. Frankel EN, Waterhouse AL, Teissedre PL. (1995) Principal phenolic phytochemicals in selected California wines and their antioxidant activity inhibiting oxidation of human low-density lipoprotein. J. Agric. Food Chem. 43, 890-894 https://doi.org/10.1021/jf00052a008
  10. Ghani A. (2003) Medicinal plants of Bangladesh with chemical constituents and uses. 2nd ed. Asiatic society of Bangladesh, p. 208-209
  11. Gordon MH. (1990). The mechanism of the antioxidant action in vitro. In: Hudson BJF (ed.), Food Antioxidants. London: Elsevier, p. 1-18
  12. Grice HC. (1986). Safety evaluation of butylated hydroxytoluene (BHT) in the liver, lung and gastrointestinal tract. Food Chem. Toxicol. 24, 1127-1130 https://doi.org/10.1016/0278-6915(86)90298-X
  13. Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI. (2002) On the in vitro antioxidant properties of melatonin. J. Pineal Res. 33, 167-171 https://doi.org/10.1034/j.1600-079X.2002.20920.x
  14. Gutteridge JM, Halliwell B. (2000) Free radicals and antioxidants in the year 2000: a historical look to the future. Ann. N.Y. Acad. Sci. 899, 136-147 https://doi.org/10.1111/j.1749-6632.2000.tb06182.x
  15. Gyamfi MA, Aniya Y. (2002) Antioxidant properties of Thonningianin A, isolated from the African medicinal herb, Thonningia sanguine. Biochem. Pharmacol. 63, 1725-1737 https://doi.org/10.1016/S0006-2952(02)00915-2
  16. Halliwell B. (1996) Antioxidants in human health and disease. Annu. Rev. Nutr. 16, 33-50 https://doi.org/10.1146/annurev.nu.16.070196.000341
  17. Hasan MS, Ahmed MI, Mondal S, Uddin SJ, Masud MM, Sadhu SK, Ishibashi M. (2006) Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitrin as the major component. Orient. Pharm. Exp. Med. 6, 355-360 https://doi.org/10.3742/OPEM.2006.6.4.355
  18. Hettiarachchy NS, Glenn KC, Gnanasambandam R, Johnson MG. (1996) Natural antioxidant extract from fenugreek (Trigonella foenumgraecum) for ground beef patties. J. Food Sci. 61, 516-519 https://doi.org/10.1111/j.1365-2621.1996.tb13146.x
  19. Ito N, Fukushima S, Hasegawa A, Shibata M, Ogiso T. (1983) Carcinogenicity of butylated anisole in F344 rats, J. Natl. Cancer Inst. 70, 343-347
  20. Kumaran A, Karunakaran AJ. (2007) In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT 40, 344-352 https://doi.org/10.1016/j.lwt.2005.09.011
  21. Kayano S, Kikuzaki H, Fukutsuka N, Mitani T, Nakatani N. (2002) Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist, J. Agric. Food Chem. 50, 3708-3712 https://doi.org/10.1021/jf0200164
  22. Kourounakis AP, Galanakis D, Tsiakitzis K. (1999) Synthesis and pharmacological evaluation of novel derivatives of anti-inflammatory drugs with increased antioxidant and anti-inflammatory activities. Drug Dev. Res. 47, 9-16 https://doi.org/10.1002/(SICI)1098-2299(199905)47:1<9::AID-DDR2>3.0.CO;2-9
  23. Lim SN, Cheung PCK, Ooi VEC, Ang PO. (2002) Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum, J. Agric. Food Chem. 50, 3862-3866 https://doi.org/10.1021/jf020096b
  24. Oyaizu M. (1986) Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  25. Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J, Codina C. (2002). Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. J. Agric. Food Chem. 50, 6882-6890 https://doi.org/10.1021/jf020540a
  26. Pavanasasivam G, Sultanbawa MUS. (1975) Chemical investigation of ceylonese plants, Part XII. (+)- 3,4',5,7-Tetrahydroxy-3'-methoxyflavanone [(+)-dihydroisorhamnetin] and 3,5,7-trihydroxy-3',4'-dimethoxyflavone (dillenetin): two new natural products from Dillenia indica L. J. Chem. Soc. Perkin Trans. 1, 612-613
  27. Prieto P, Pineda M, Aguilar M. (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal. Biochem. 269, 337-341 https://doi.org/10.1006/abio.1999.4019
  28. Sherwin ER. (1978) Oxidation and antioxidants in fat and oil processing. J. Am. Oil. Chem. Soc. 55, 809-814 https://doi.org/10.1007/BF02682653
  29. Singelton VR, Orthifer R, Lamuela-Raventos RM. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymol. 299, 152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  30. Svilaas A, Sakhi AK, Andersen LF, Svilaas T, Strom EC, Jacobs DR Jr, Ose L, Blomhoff R. (2004) Intakes of antioxidants in coffee, wine and vegetables are correlated with plasma carotenoids in humans. J. Nutr. 134, 562-567 https://doi.org/10.1093/jn/134.3.562
  31. Vinson JA, Hao Y, Zubic SK (1998) Food antioxidant quantity and quality in foods: Vegetables. J. Agric. Food Chem. 46, 3630-3634 https://doi.org/10.1021/jf980295o
  32. Viturro C, Molina A, Schmeda-Hischmann G. (1999) Free radical scavengers from Mutisia friesiana (Asteraceae) and Sanicula graveolens (Apiaceae). Phytother. Res. 13, 422-424 https://doi.org/10.1002/(SICI)1099-1573(199908/09)13:5<422::AID-PTR462>3.0.CO;2-M
  33. Wichi HP. (1988) Enhanced tumor development by butylated hydroxyanisole (BHA) from the prospective of effect on forestomach and oesophageal aquamous epithelium. Food Chem. Toxicol. 26, 717-723 https://doi.org/10.1016/0278-6915(88)90072-5
  34. Wild AC, Mulcahy RT. (2000) Regulation of gammaglutamylcysteine synthetase subunit gene expression: insights into transcriptional control of antioxidant defenses. Free Radic. Res. 32, 281-301 https://doi.org/10.1080/10715760000300291
  35. Yokozawa T, Chen CP , Dong E, Tanaka T, Nonaka GI, Nishioka I. (1998) Study on the inhibitory effect of tannins and flavonoids against the 1,1-Diphenyl-2-picrylhydrazyl radical. Biochem. Pharmacol. 56, 213-222 https://doi.org/10.1016/S0006-2952(98)00128-2

피인용 문헌

  1. Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India vol.7, pp.1664-462X, 2016, https://doi.org/10.3389/fpls.2016.00407